Rapid preparation of giant unilamellar vesicles. [PDF]
Proceedings of the National Academy of Sciences, 1996 We report here a rapid evaporation method that produces in high yield giant unilamellar vesicles up to 50 microns in diameter. The vesicles are obtained after only 2 min and can be prepared from different phospholipids, including L-alpha-phosphatidylcholine (lecithin), dipalmitoleoyl L-alpha-phosphatidylcholine, and beta-arachidonoyl gamma-palmitoyl L ...
Alexander Moscho +4 more
openalex +3 more sources
Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles [PDF]
ACS OmegaGarvita Dhanawat +3 more
doaj +2 more sources
In Situ Assembly of Transmembrane Proteins from Expressed and Synthetic Components in Giant Unilamellar Vesicles. [PDF]
ACS Chem Biol, 2022 Podolsky KA +4 more
europepmc +3 more sources
Division and Regrowth of Phase-Separated Giant Unilamellar Vesicles*. [PDF]
Angew Chem Int Ed Engl, 2021 AbstractSuccess in the bottom‐up assembly of synthetic cells will depend on strategies for the division of protocellular compartments. Here, we describe the controlled division of phase‐separated giant unilamellar lipid vesicles (GUVs). We derive an analytical model based on the vesicle geometry, which makes four quantitative predictions that we verify
Dreher Y +4 more
europepmc +7 more sources
Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles. [PDF]
ACS Synth Biol, 2021 Van de Cauter L +9 more
europepmc +2 more sources
Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes [PDF]
ACS Omega, 2017 Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively monodisperse, defect-free vesicles.
Valerio Pereno +6 more
doaj +9 more sources
Translocation of silica nanospheres through giant unilamellar vesicles (GUVs) induced by a high frequency electromagnetic field. [PDF]
RSC Adv, 2021 Tharushi Perera PG +9 more
europepmc +3 more sources
Hierarchical unilamellar vesicles of controlled compositional heterogeneity. [PDF]
PLoS ONE, 2012 Eukaryotic life contains hierarchical vesicular architectures (i.e. organelles) that are crucial for material production and trafficking, information storage and access, as well as energy production. In order to perform specific tasks, these compartments
Maik Hadorn +3 more
doaj +1 more source
Asymmetric Giant Unilamellar Vesicles [PDF]
Biophysical Journal, 2010 The lipid composition of the eukaryotic plasma membrane is asymmetric; that is, if the bilayer is considered to consist of two leaflets, the outer-facing leaflet contains different lipids at different concentrations than the inner-facing leaflet. While there has been much speculation as to the physiological purpose of this asymmetry, it has been ...
Peichi C. Hu, Noah Malmstadt
openaire +2 more sources
Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining
Bio-Protocol, 2023 Loss of plasma membrane lipid asymmetry contributes to many cellular functions and responses, including apoptosis, blood coagulation, and cell fusion.
Julia Baum +2 more
doaj +1 more source