Results 41 to 50 of about 7,136 (241)

Toxicity of cryoprotective agents at 30°

Journal of Pharmacy and Pharmacology, 1968
Abstract The toxicity of four cryoprotective agents (glycerol, dimethylsulfoxide, dextran, magnesium ion) as a function of time was assessed at 30° on isolated rabbit atria. Glycerol, 2·0 M, in Ringer solution killed all atria in less than 30 min. Atria tolerated 2·1 M dimethylsulphoxide in Ringer for 1 hr, 0·7 M glycerol in Ringer for 4
Ben R. Clower, Oliver Carrier, Armand M. Karow   +2 more
openaire   +2 more sources

Sonochemical application reduces monosaccharide levels and improves cryoprotective effect of Jerusalem artichoke extract on Leuconostoc mesenteroides WiKim33 during freeze-drying

Ultrasonics Sonochemistry, 2023
Lactic acid bacteria (LAB) are being used for probiotic and starter cultures to prevent global damage to microbial cells. To retain the benefits of LAB in the commercially used powdered form, highly efficient cryoprotective agents are required during the
Yeong Yeol Kim, Ho Myeong Kim, Seul-Gi Jeong, Jung Eun Yang, Seulbi Kim, Hae Woong Park   +5 more
doaj  

EFFECT OF DIFFERENT CRYOPROTECTIVE AGENTS ON SKIM MILK AND DIMITROPOULUS EXTENDER FOR STALLION SEMEN CRYOPRESERVATION

Journal of the Indonesian Tropical Animal Agriculture, 2014
s to assess different CPAs on stallion semen cryopreservation. Skim milk (SM) and Dimitropoulos(DV) were the extenders used in this study; each was added by glycerol (Gly), combination of ethyleneglycol-glycerol (EG+Gly) or dimethilformamide (DMF).
R.I. Arifiantini, B. Purwantara, T.I. Yusuf, D. Sajuthi   +3 more
doaj   +1 more source

Comparison of different cryoprotective agents on swamp buffalo semen cryopreservation

Journal of the Indonesian Tropical Animal Agriculture, 2020
Present study aimed to determine the effectivenessof dimethylformamide (DMF)as cryoprotective agent (CPA) by determining its optimum concentration inTris Eggyolk (TEY) extender on the quality of post-thaw swamp buffalo semen. Only ejaculates having > 60%
M. T. Herbowo, R. I. Arifiantini, N. W. K. Karja, R. G. Sianturi   +3 more
doaj   +1 more source

Cryopreservation: Methods, equipment and critical concerns [PDF]

, 2016
The technique of cryopreservation aims to maintain the biological samples in a metabolic suspension state, by which all chemical, physical and biological changes are diminished.
Herrmann, J, Jouhara, H, Nannou, TK, Trembley, J   +3 more
core   +1 more source

Differentiation of Human iPS Cells Into Sensory Neurons Exhibits Developmental Stage-Specific Cryopreservation Challenges

Frontiers in Cell and Developmental Biology, 2021
Differentiation of human induced pluripotent stem cells (hiPSCs) generates cell phenotypes valuable for cell therapy and personalized medicine. Successful translation of these hiPSC-derived therapeutic products will rely upon effective cryopreservation ...
Rui Li, Patrick Walsh, Vincent Truong, Ashley Petersen, James R. Dutton, James R. Dutton, Allison Hubel, Allison Hubel   +7 more
doaj   +1 more source

Cryopreservation and its clinical applications

Integrative Medicine Research, 2017
Cryopreservation is a process that preserves organelles, cells, tissues, or any other biological constructs by cooling the samples to very low temperatures.
Tae Hoon Jang, Sung Choel Park, Ji Hyun Yang, Jung Yoon Kim, Jae Hong Seok, Ui Seo Park, Chang Won Choi, Sung Ryul Lee, Jin Han   +8 more
doaj   +1 more source

Me2SO perfusion time for whole-organ cryopreservation can be shortened: Results of micro-computed tomography monitoring during Me2SO perfusion of rat hearts.

PLoS ONE, 2020
Cryopreservation of whole organs and specific tissues is an important and continually expanding field of medicine. The protocols currently used for organ preservation do not ensure survivability and functionality; the protocols for ovarian tissue lead to
Nathalie Bleisinger, Ralf Dittrich, Olga Strahl, Robert Brauweiler, Inge Hoffmann, Matthias W Beckmann, Tilmann Volk   +6 more
doaj   +1 more source

The Role of Aquaporin 7 in the Movement of Water and Cryoprotectants in Bovine In Vitro Matured Oocytes

Animals, 2022
Aquaglyceroporins are known as channel proteins, and are able to transport water and small neutral solutes. In this study, we evaluate the effect of exposure of in vitro matured bovine oocytes to hyperosmotic solutions containing ethylene glycol (EG ...
Tania García-Martínez, Iris Martínez-Rodero, Joan Roncero-Carol, Meritxell Vendrell-Flotats, Jaume Gardela, Alfonso Gutiérrez-Adán, Priscila Ramos-Ibeas, Adam Z. Higgins, Teresa Mogas   +8 more
doaj   +1 more source

Effects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions

, 2006
Vitrification of aqueous cryoprotectant mixtures is essential in cryopreservation of proteins and other biological samples. We report systematic measurements of critical cryoprotective agent (CPA) concentrations required for vitrification during plunge ...
Alsaied, Osama A., Berejnov, Viatcheslav, Husseini, Naji S., Thorne, Robert E.   +3 more
core   +1 more source