Lipid Lateral Organization on Giant Unilamellar Vesicles Containing Lipopolysaccharides [PDF]
We developed a new (to our knowledge) protocol to generate giant unilamellar vesicles (GUVs) composed of mixtures of single lipopolysaccharide (LPS) species and Escherichia coli polar lipid extracts. Four different LPSs that differed in the size of the polar headgroup (i.e., LPS smooth > LPS-Ra > LPS-Rc > LPS-Rd) were selected to generate GUVs composed
Jakub Kubiak+3 more
openalex +6 more sources
Chemical communication at the synthetic cell/living cell interface
Synthetic models of cells are becoming increasingly sophisticated, but engineering communication between these and living cells remains challenging. Here the authors review modes of communication and signal processing between living cells and synthetic ...
Vincent Mukwaya+2 more
doaj +1 more source
Gel-Assisted Formation of Giant Unilamellar Vesicles [PDF]
Giant unilamellar vesicles or GUVs are systems of choice as biomimetic models of cellular membranes. Although a variety of procedures exist for making single walled vesicles of tens of microns in size, the range of lipid compositions that can be used to grow GUVs by the conventional methods is quite limited, and many of the available methods involve ...
Weinberger, Andreas+8 more
openaire +3 more sources
Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells
Controlled actuation is an important aspect of synthetic cellular systems. Here, the authors combine pH responsive DNA origami structures with light triggered proton pump engineered E.
Kevin Jahnke+11 more
doaj +1 more source
Block Copolymer Giant Unilamellar Vesicles for High-Throughput Screening
Bottom-up synthetic cells offer the potential to study cellular processes with reduced complexity. Giant unilamellar vesicles (GUVs) can mimic cells in their morphological characteristics because their architecture is precisely controllable.
Lukas Heuberger, Cornelia Palivan
doaj +1 more source
Magnetoliposomes as model for signal transmission [PDF]
Liposomes containing magnetic nanoparticles (magnetoliposomes) have been extensively explored for targeted drug delivery. However, the magnetic effect of nanoparticles movement is also an attractive choice for the conduction of signals in communication ...
G. R. Barreto+8 more
doaj +1 more source
Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy [PDF]
A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values
arxiv +1 more source
Thermodynamic relaxation drives expulsion in giant unilamellar vesicles [PDF]
We investigated the thermodynamic relaxation of giant unilamellar vesicles (GUVs) which contained small vesicles within their interior. Quenching these vesicles from their fluid phase (T>Tm) through the phase transition in the gel state ...
Leirer, C. T.+3 more
openaire +5 more sources
Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays
To execute higher-order functions, cells self-assemble into spatially coded tissue configurations. Here the authors magnetically assembly giant unilamellar vesicles into three dimensional tissue-mimic structures with collective osmotic stability.
Qingchuan Li+4 more
doaj +1 more source
Thermal fluctuations and osmotic stability of lipid vesicles [PDF]
Biological membranes constantly change their shape in response to external stimuli, and understanding the remodeling and stability of vesicles in heterogeneous environments is therefore of fundamental importance for a range of cellular processes. One crucial question is how vesicles respond to external osmotic stresses, imposed by differences in solute
arxiv +1 more source