Predictive Monitoring of Shake Flask Cultures with Online Estimated Growth Models [PDF]
Bioengineering, 2021 Simplicity renders shake flasks ideal for strain selection and substrate optimization in biotechnology. Uncertainty during initial experiments may, however, cause adverse growth conditions and mislead conclusions.
Barbara Pretzner +5 more
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Systematic evaluation of characteristics of the membrane-based fed-batch shake flask [PDF]
Microbial Cell Factories, 2017 Background The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions.
P. Philip +6 more
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Analysis and effect of conventional flasks in shaking culture of Escherichia coli [PDF]
AMB Express, 2020 The circulation direct monitoring and sampling system (CDMSS) is used as a monitoring device for CO2 and O2 concentrations of bypass type in shake-culture flask.
Masato Takahashi, Hideki Aoyagi
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High cell density cultivation of Bacillus subtilis NCIM 2063: Modeling, optimization and a scale-up procedure [PDF]
Journal of the Serbian Chemical Society, 2023 Bacillus subtilis is a non-pathogenic, sporulating, Gram-positive bacteria with pronounced antimicrobial and metabolic activity and great potential for wide application in various fields. The aim of this paper was to determine the optimum B.
Stamenković-Stojanović Sandra +4 more
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Brazilian Journal of Pharmaceutical Sciences, 2021 Among the methods described for determining the solubility, shake-flask is suitable to evaluate the equilibrium solubility according to the BCS. Nevertheless, experimental conditions related to the shake-flask method are not well described.
Patrícia Fernanda Monteiro +5 more
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Effect of Shaking Speed and Type of Closure on Shake Flask Cultures [PDF]
Applied Microbiology, 1969 Growth of microorganisms and biosynthesis of microbial products in shake flasks may be limited by operating conditions which provide inadequate supplies of oxygen. Methods are described for meeting the oxygen requirements of test organisms by using standard baffled flasks with pad-type closures and shaking at relatively high speeds. Growth
L. E. McDaniel, E. G. Bailey
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Shipin gongye ke-ji, 2023 Objective: In this study, Corynebacterium glutamicum ATCC 13032 was used as the chassis cell for synthesizing L-homoserine and analyzing the effect of dissolved oxygen on product synthesis. Methods: First, the product tolerance of C.
Qiushuang GUO +6 more
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Levan Production in Shake Flask and Fermenter Influence of Feeding Strategy on Levan Yield and Molecular Weight Distribution [PDF]
E3S Web of Conferences, 2023 Effect of feeding strategy on levan production was studied in a shake flask and a 5 L lab scale fermenter. In a shake flask, levan specific substrate yield (YP/S) increased from 0.35 g levan/g sucrose to 0.48 g levan/g sucrose with repeated batch feeding.
Veerapandian Bhuvaneshwari +5 more
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View on a mechanistic model of Chlorella vulgaris in incubated shake flasks [PDF]
Bioprocess and Biosystems Engineering, 2021 Abstract Kinetic growth models are a useful tool for a better understanding of microalgal cultivation and for optimizing cultivation conditions. The evaluation of such models requires experimental data that is laborious to generate in bioreactor settings. The experimental shake flask setting used in this study allows to
Fabian Kuhfuß +5 more
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Breeding of a High-Yield Glutathione-Producing Strain by Atmospheric and Room Temperature Plasma Mutagenesis and Microbial Microdroplet Culture System [PDF]
Shipin Kexue, 2023 In order to improve the ability of a wild-type strain (BY-1) of Pichia pastoris to produce glutathione (GSH), a mutant strain designated BY-1-26 was obtained by atmospheric and room temperature plasma (ARTP) mutagenesis. To further improve the production
LING Siyu, WANG Zhou, ZHANG Huimin, LI Chuang, LIU Qingtao, LIU Yan, XUE Zhenglian
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