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Western Blot tekniği, herhangi bir örnekten istenen tek bir proteini özgün bir şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir bir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik protein izoformlarının ...
Hatice Pelin ASLIM, Oya BULUT
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BlotIt-Optimal alignment of Western blot and qPCR experiments. [PDF]
Biological systems are frequently analyzed by means of mechanistic mathematical models. In order to infer model parameters and provide a useful model that can be employed for systems understanding and hypothesis testing, the model is often calibrated on ...
Svenja Kemmer+4 more
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Measurement of Antigen by Enhanced Chemiluminescent Western Blot
Donghui Huang, Sally A. Amero
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BACKGROUND: Congenital toxoplasmosis is an infection caused by the Toxoplasma gondii a protozoa obligate intracellular parasite that appears after a mother´s primary infection occurs during pregnancy.
Sebastián Serna Rivera+5 more
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A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples
Despite the availability of a wide range of commercial kits, protein quantification is often unreliable, especially for tissue-derived samples, leading to uneven loading in subsequent experiments. Here we show that the widely used Bicinchoninic Acid (BCA)
Dario Lucas Helbing+4 more
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IntroductionThe environmental bacterium Burkholderia pseudomallei causes the often fatal and massively underreported infectious disease melioidosis.
Gabriel E. Wagner+13 more
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Aptamer Validation by Western Blot–an overview
Western blot is the main and basic technique in cellular and molecular biology. The principle of the western blot is the isolation and detection of the target molecule usually from a cellular extract.
Lamees Jamal Talib +2 more
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Background: Echinococcosis is a common parasite with zoonotic character created by a small cestode, Echinococcus spp., and is an important public health problem in Turkey as well as all over the world. We aimed to investigate antibodies in serum samples
Banuçiçek Yücesan+3 more
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Development and application of two novel monoclonal antibodies against overexpressed CD26 and integrin α3 in human pancreatic cancer. [PDF]
Monoclonal antibody (mAb) technology is an excellent tool for the discovery of overexpressed cell surface tumour antigens and the development of targeting agents.
Arias-Pinilla, GA+5 more
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