Results 121 to 130 of about 1,209,776 (284)
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 摘 要： 【目的】 探讨同型半胱氨酸（Ｈｃｙ）对外周血内皮祖细胞（EPC）迁移、粘附能力的影响。【方法】密度梯度离心法获取单个核细胞，接种于铺有人纤维连接蛋白包被的培养皿，培养７ ｄ后免疫荧光鉴定EPC。实验分对照组（Ｃｏｎｔｒｏｌ，ＣＴ）、５０ μｍｏｌ／Ｌ腺苷（ａｄｅｎｏｓｉｎｅ， Ａｄｅ）组（Ａ５０）、５０ μｍｏｌ／Ｌ Ｈｃｙ组（Ｈ５０）、５０ μｍｏｌ／Ｌ Ｈｃｙ＋５０μｍｏｌ／ＬＡｄｅ组（Ａ５０＋Ｈ５０）和５０ μｍｏｌ／Ｌ Ｈｃｙ＋５０ μｍｏｌ／Ｌ Ａｄｅ＋１０ ｎｇ ...
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Zhongshan Daxue xuebao. Yixue kexue ban, 2014 【目的】 探讨320排动态容积CT血管造影对评估糖尿病眼动脉硬化的临床价值?【方法】对30例非糖尿病患者和22例糖尿病患者进行320排动态容积CT血管造影检查,对所有扫描原始数据行最大密度投影(MIP)?容积再现(VR)?多平面重建(MRP)及曲面重建(CPR),观察眼动脉的管腔情况?【结果】 320排动态容积CT血管造影检查可以清晰显示眼动脉形态?走行,以及病变的部位?情况?22例糖尿病患者血管呈不同程度粥样硬化表现?其中颅内段轻度狭窄6侧,中度狭窄4侧;视神经管内段轻度狭窄2侧,中度狭窄4侧 ...
李庆玲 +5 more
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Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
沉默Ｐｏｋｅｍｏｎ基因对鼻咽癌细胞株ＣＮＥ－２放射敏感性的影响
Zhongshan Daxue xuebao. Yixue kexue ban, 2012 【目的】 探讨沉默Ｐｏｋｅｍｏｎ基因对鼻咽癌细胞（ＣＮＥ-２）放射敏感性的影响，寻求放射治疗鼻咽癌的新方法。 【方法】 首先利用ｓｉＲＮＡ预测软件得到３条ｓｉＲＮＡ序列，并制备合成ｓｉＲＮＡ片段，利用ＲＴ-ＰＣＲ和免疫印迹法检测其沉默Ｐｏｋｅｍｏｎ基因的效果，筛选出沉默效果最佳的ｓｉＲＮＡ片段，转染ＣＮＥ-２细胞后观察其对ＣＮＥ-２细胞ｐ５３基因表达的影响、ＭＴＴ法检测细胞的增殖活性、流式细胞术检测细胞的凋亡、克隆形成法检测转染ｓｉＲＮＡ联合放射处理后细胞的放射增敏比。【结果 ...
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FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
肥胖儿童血清omentin-1和vaspin水平及其与脂代谢的关系
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 【目的】 探讨肥胖儿童血清omentin-1?vaspin水平及其与脂代谢的关系?【方法】 59名儿童参加本研究,肥胖组30人,年龄(9.43 ± 2.02)岁,对照组29人,年龄(10.3 ± 2.2)岁,两组年龄无显著差异(P > 0.05)?采用ELISA法检测血清omentin-1和vaspin水平?采用生化检测测定空腹血脂和空腹血糖?采用化学发光法检测空腹胰岛素?计算每个个体的体质量指数(BMI)?体质量指数Z积分(BMI-SDS)及胰岛素抵抗指数(HOMA-IR)?【结果】 肥胖组的BMI ...
doaj
FEBS Letters, EarlyView.Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley +1 more source
苯磺酸左旋氨氯地平对2型糖尿病合并高血压患者血小板聚集及MMP-9/MMP-2表达的影响
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 【目的】 观察苯磺酸左旋氨氯地平对2型糖尿病合并高血压患者血小板聚集率(PAG)的影响,并探讨其血小板抑制作用是否通过改变血小板释放MMP-9和MMP-2的途径实现?【方法】 体外测定32例2型糖尿病合并高血压患者的血小板经苯磺酸左旋氨氯地平处理前后聚集率的变化,同时用ELISA法测定MMP-9和MMP-2表达的变化?【结果】 经苯磺酸左旋氨氯地平处理后,2型糖尿病合并高血压患者血小板的聚集率从(47.77 ± 11.92)%下降至(40.78 ± 13.97)%,P < 0.05,抑制率为(13.50
doaj
, 2009 Denne oppgaven har hatt som mål å kartlegge hvordan skoleledelsen kan legge til rette for å utvikle en digitalt kompetent skole Utgangspunktet har vært en kvalitativ studie av to demonstrasjonsskoler som av myndighetene er anerkjente for sitt IKT‐arbeid og for å vise tegn på å være lærende organisasjoner.
openaire +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source