Results 81 to 90 of about 24,599 (245)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

The Early Chalcolithic and Late Chalcolithic at Sultana–Ghețărie site (southern Romania). A look from the pots

open access: yesБългарско е-Списание за Археология
This study examines two prehistoric pottery assemblages from the Sultana–Ghețărie site (southern Romania), excavated in 2021 from distinct contexts: an Early Chalcolithic pit (Boian–Vidra tradition, ca.
Vasile Opriș, Robert Hofmann
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

The Functions of Theology to Christian Tradition

open access: yesComparative Theology, 2013
The term Theology has various definitions and meanings in the Christian tradition. In this article, after discussing these definitions and meanings we have tried to illuminate the extended functions of theology in Christianity and we have also explained the meaning of this term which has been entered to our literature by means of translation from ...
Ghasem Kakaie, Ashkan Bahrani
openaire   +2 more sources

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Shaolin Physical Conditioning: What’s Old is New Again

open access: yesRevista de Artes Marciales Asiáticas, 2012
In recent years, there has been a resurgence of functional fitness training that was practiced in the ancient Shaolin Temple as part of their regular physical conditioning regimen. This type of training—done with equipment (iron rings, bamboo, vases, etc.
Julio Anta
doaj  

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

open access: yesFEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss   +7 more
wiley   +1 more source

“Скиты” веселой Елизавет: Русские эрмитажи середины XVIII века

open access: yesВивліоѳика
The article is devoted to the history of the appearance of garden pavilions and Russian palace chambers also known as "hermitages." The tradition of building hermitages as special-purpose premises, adopted in Russia in the early eighteenth century, had ...
Анна Корндорф
doaj   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

open access: yesFEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan   +11 more
wiley   +1 more source

Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model

open access: yesFEBS Open Bio, EarlyView.
This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito   +7 more
wiley   +1 more source

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