Results 31 to 40 of about 626,384 (268)

Thermostable α-galactosidase fromBacillus stearothermophilusNUB3621: cloning, sequencing and characterization [PDF]

FEMS Microbiology Letters, 1999
An alpha-galactosidase gene from the thermophilic bacterium Bacillus stearothermophilus NUB3621 was cloned, sequenced, expressed in Escherichia coli and the recombinant protein was purified. The Bacillus enzyme, designated AgaN, is similar to alpha-galactosidases of family 36 in the classification of glycosyl hydrolases.
O, Fridjonsson, H, Watzlawick, A, Gehweiler, R, Mattes   +3 more
openaire   +2 more sources

Anaerobic expression of thegyrAgene demonstrated by β-galactosidase activity ingyrA::Mu-lacfusion derivatives ofEscherichia coli [PDF]

FEMS Microbiology Letters, 1988
E. coli C600Δ lac was infected with a Mu- lac defective phage. Lysogens resistant to ampicillin (25 µg/ml) and nalidixic acid (20 µg/ml) were unable to grow anaerobically and insensitive to nalidixic acid (150 µg/ml), indicating formation of gyrA ::Mu- lac fusions.
Nobuto Yamamoto, Mary L. Droffner
openaire   +1 more source

Dysregulated DNA methylation in the pathogenesis of Fabry disease

Molecular Genetics and Metabolism Reports, 2022
Fabry disease is an X-linked lysosomal storage disorder caused by a deficiency of α-galactosidase A and subsequent accumulation of glycosphingolipids with terminal α-D-galactosyl residues.
Jin-Song Shen, Uthra Balaji, Kunitoshi Shigeyasu, Yoshinaga Okugawa, Siamak Jabbarzadeh-Tabrizi, Taniqua S. Day, Erland Arning, John Marshall, Seng H. Cheng, Jinghua Gu, Raphael Schiffmann, Teodoro Bottiglieri, Ajay Goel   +12 more
doaj   +1 more source

Experimental conditions may affect reproducibility of the beta-galactosidase assay [PDF]

FEMS Microbiology Letters, 1992
Several experimental conditions and parameters contributing to the determination of beta-galactosidase activity, as proposed in Miller's assay, were studied. Use of the absorbance correction factor and the nature and concentration of permeabilizing agents were taken into account as different experimental conditions.
GIACOMINI, ALESSIO, CORICH, VIVIANA, F. J. Ollero, SQUARTINI, ANDREA, M. P. Nuti   +4 more
openaire   +2 more sources

Evaluation of beta-galactosidase activity in tissue in the presence of blood [PDF]

, 2000
The reporter gene for beta -galactosidase is frequently used to determine the efficiency of gene transfer in arteries. However, blood is often present in arterial explants and may compromise the results by the presence of hemoglobin. The light absorption
Pelisek, Jaroslav, Nikol, Sigrid, Armeanu, Sorin   +2 more
core   +1 more source

Identification of an intestine-specific promoter and inducible expression of bacterial α-galactosidase in mammalian cells by a lac operon system

Journal of Animal Science and Biotechnology, 2012
Background α-galactosidase has been widely used in animal husbandry to reduce anti-nutritional factors (such as α-galactoside) in feed. Intestine-specific and substrate inducible expression of α-galactosidase would be highly beneficial for transgenic ...
Ya-Feng Zhai, Gang Shu, Xiao-Tong Zhu, Zhi-Qi Zhang, Xia-Jing Lin, Song-Bo Wang, Li-Na Wang, Yong-Liang Zhang, Qing-Yan Jiang   +8 more
doaj   +1 more source

Cloning of the phospho-β-galactosidase gene inEscherichia colifrom lactose-negative mutants ofStreptococcus mutansisolated following random mutagenesis with plasmid pVA891 clone banks [PDF]

FEMS Microbiology Letters, 1992
In order to mutagenize Streptococcus mutans a marker rescue plasmid, pVA891, was employed. The plasmid was ligated with Sau3AI digested chromosomal DNA fragments from S. mutans GS-5IS3 and the resultant plasmids were amplified in Escherichia coli.
Yutaka Sato, Yasuhito Yamamoto, Harutoshi Kizaki, Howard K. Kuramitsu   +3 more
openaire   +1 more source

Nicotiana benthamiana α-galactosidase A1.1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease. [PDF]

J Biol Chem, 2018
α-Galactosidases (EC 3.2.1.22) are retaining glycosidases that cleave terminal α-linked galactose residues from glycoconjugate substrates. α-Galactosidases take part in the turnover of cell wall-associated galactomannans in plants and in the lysosomal ...
Kytidou K, Beekwilder J, Artola M, van Meel E, Wilbers RHP, Moolenaar GF, Goosen N, Ferraz MJ, Katzy R, Voskamp P, Florea BI, Hokke CH, Overkleeft HS, Schots A, Bosch D, Pannu N, Aerts JMFG.   +16 more
europepmc   +3 more sources

Characterization and molecular cloning of a heterodimeric β-galactosidase from the probiotic strainLactobacillus acidophilusR22 [PDF]

FEMS Microbiology Letters, 2007
Beta-galactosidase from the probiotic strain Lactobacillus acidophilus R22 was purified to apparent homogeneity by ammonium sulphate fractionation, hydrophobic interaction, and affinity chromatography. The enzyme is a heterodimer consisting of two subunits of 35 and 72 kDa, as determined by gel electrophoresis.
Thu-Ha, Nguyen, Barbara, Splechtna, Stanimira, Krasteva, Wolfgang, Kneifel, Klaus D, Kulbe, Christina, Divne, Dietmar, Haltrich   +6 more
openaire   +2 more sources

Residual α-galactosidase A activity in Fabry males and females. [PDF]

, 2022
Activity is expressed, as nmol/h/mg protein. Grey area represents the normal α-galactosidase A activity range (20–65 nmol/h/mg protein). Available measurements in n = 35/39 males and females n = 61/76 females. ****: p-value
Ulla Feldt-Rasmussen (317604), Mette Mogensen (6059357), Vitor Hugo Fraga de Abreu (14129178), Tatiana Kristensen (14129184), Allan M. Lund (11933730), Caroline Kistorp (2993424), Åse Krogh Rasmussen (8098304), Lotte Hahn Enevoldsen (14129181), Mads Klokker (669761), Flemming Wibrand (4591960), Lars Kober (12873158), Peter Oturai (647627), Henning Bundgaard (441832), Vibeke Backer (764121), Morten Dunoe (14129163), Farah Arif (14129193), Soren S. Sorensen (14129169), Puriya D. W. Yazdanfard (14129172), Margrethe Bastholm Bille (14129190), Vibeke A. Larsen (14129175), Lis F. Hasholt (14129166), Grigoris Effraimidis (2617039), Kirsten Svenstrup (14129187)   +22 more
core   +1 more source