Cloning and characterization of a novel α-galactosidase fromBifidobacterium breve203 capable of synthesizing Gal-α-1,4 linkage [PDF]
FEMS Microbiology Letters, 2008 A novel alpha-galactosidase gene (aga2) was cloned from Bifidobacterium breve 203. It contained an ORF of 2226-bp nucleotides encoding 741 amino acids with a calculated molecular mass of 81.5 kDa. The recombinant enzyme Aga2 was heterogeneously expressed, purified and characterized. Regarding substrate specificity for hydrolysis, Aga2 was highly active
Han, Zhao +8 more
openaire +2 more sources
Plasma mutant α-galactosidase A protein and globotriaosylsphingosine level in Fabry disease
Molecular Genetics and Metabolism Reports, 2014 Fabry disease is an X-linked genetic disorder characterized by deficient activity of α-galactosidase A (GLA) and accumulation of glycolipids, and various GLA gene mutations lead to a wide range of clinical phenotypes from the classic form to the later ...
Takahiro Tsukimura +7 more
doaj +1 more source
Hepatology, EarlyView., 2022 Abstract Background and Aims Intrahepatic cholangiocarcinoma (ICC) is a deadly but poorly understood disease, and its treatment options are very limited. The aim of this study was to identify the molecular drivers of ICC and search for therapeutic targets.
Yuto Shiode +16 more
wiley +1 more source
Fabry disease, a complex pathology not easy to diagnose
Cardiogenetics, 2015 Fabry disease is a multisystemic lysosomal storage disorder, inherited in an X-linked manner. It is a defect of metabolism of the glycosphingolipids, due to the reduction or absence of the activity of lysosomal enzyme α-galactosidase A. This reduction of
Paolo Colomba +8 more
doaj +1 more source
Improving the Secretory Expression of an -Galactosidase from Aspergillus niger in Pichia pastoris. [PDF]
PLoS ONE, 2016 α-Galactosidases are broadly used in feed, food, chemical, pulp, and pharmaceutical industries. However, there lacks a satisfactory microbial cell factory that is able to produce α-galactosidases efficiently and cost-effectively to date, which prevents ...
Xianliang Zheng +6 more
doaj +1 more source
Enzyme Replacement Therapy in the Treatment of Fabry Disease
罕见病研究, 2023 Fabry disease is an X-linked inherited lysosomal storage disease caused by the mutation of GLA gene that encodes α-galactosidase A (α-Gal A). GLA gene mutation causes the decline or deficiency in the activity of α-Gal A, leading to the accumulation of ...
YU Che, WANG Rong
doaj +1 more source
Identification of bacteria with β-galactosidase activity in faeces from lactase non-persistent subjects [PDF]
FEMS Microbiology Ecology, 2005 Previous studies suggest that, besides the maldigestion of lactose in the small intestine, the colonic processing of lactose might play a role in lactose intolerance. beta-Galactosidase is the bacterial enzyme which catalyzes the first step of lactose fermentation in the colon.
Tao, H +3 more
openaire +3 more sources
α-d-galactosidase deficiency in coconut endosperm: its possible pleiotropic effects in makapuno [PDF]
, 2021 α-d-galactosidase (α-d-galactoside galactohydrolase, EC 3.2.1.22) activity in the normal endosperm increased with age continually up to endosperm maturity.
Mendoza, Evelyn Mae T. +2 more
core
Production of a Highly Protease-Resistant Fungal α-Galactosidase in Transgenic Maize Seeds for Simplified Feed Processing. [PDF]
PLoS ONE, 2015 Raffinose-family oligosaccharide (RFO) in soybeans is one of the major anti-nutritional factors for poultry and livestocks. α-Galactosidase is commonly supplemented into the animal feed to hydrolyze α-1,6-galactosidic bonds on the RFOs.
Wenxia Yang +9 more
doaj +1 more source
α-Galactosidase A fromPseudomonas fluorescenssubsp.cellulosa: cloning, high level expression and its role in galactomannan hydrolysis [PDF]
FEMS Microbiology Letters, 2000 A library of Pseudomonas fluorescens subsp. cellulosa genomic DNA, constructed in lambda ZAPII, was screened for alpha-D-galactosidase activity. The DNA inserts from six galactosidase-positive clones were rescued into plasmids. Restriction digestion and Southern analysis revealed that each of the plasmids contained a common DNA sequence.
J R, Halstead +5 more
openaire +2 more sources