Lactobacillus fermentumCRL 722 is able to deliver active α-galactosidase activity in the small intestine of rats [PDF]
FEMS Microbiology Letters, 2005 alpha-galactooligosaccharides (alpha-GOS) found in legumes such as soybeans can cause gastrointestinal disorders since mammals lack alpha-galactosidase (alpha-Gal) in the small intestine which is necessary for their hydrolysis. Lactobacillus fermentum CRL 722 is a lactic acid bacterium (LAB) capable of degrading alpha-GOS due to its elevated alpha-Gal ...
Leblanc, Jean Guy +3 more
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Insertion of a 27 amino acid viral peptide in different zones ofEscherichia coliβ-galactosidase: Effects on the enzyme activity [PDF]
FEMS Microbiology Letters, 1994 Seven internal, putatively exposed regions of Escherichia coli beta-galactosidase have been explored regarding their tolerance to insertions of large foreign peptides. Small sequence modifications, including amino acid substitutions and small deletions, were introduced into the lacZ gene to generate unique BamHI restriction sites. By using these mutant
A, Benito, A, Villaverde
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Human Genomics, 2006 Fabry disease, an X-linked recessive inborn error of glycosphingolipid catabolism, results from the deficient activity of the lysosomal exoglycohydrolase, α-galactosidase A (EC 3.2.1.22; α-Gal A).
Shabbeer Junaid +3 more
doaj +1 more source
Cloning and characterization of a novel α-galactosidase fromBifidobacterium breve203 capable of synthesizing Gal-α-1,4 linkage [PDF]
FEMS Microbiology Letters, 2008 A novel alpha-galactosidase gene (aga2) was cloned from Bifidobacterium breve 203. It contained an ORF of 2226-bp nucleotides encoding 741 amino acids with a calculated molecular mass of 81.5 kDa. The recombinant enzyme Aga2 was heterogeneously expressed, purified and characterized. Regarding substrate specificity for hydrolysis, Aga2 was highly active
Han, Zhao +8 more
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Production of secreted guar α-galactosidase by Lactococcus lactis [PDF]
, 1995 A plant α-galactosidase gene was inserted in the expression vector pGKV259. The resulting plasmid pGAL2 consisted of the replication functions of the broad-host-range lactococcal plasmid pWV01, the lactococcal promoter P59, and the DNA sequences encoding
Bolhuis, A. +9 more
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Plasma mutant α-galactosidase A protein and globotriaosylsphingosine level in Fabry disease
Molecular Genetics and Metabolism Reports, 2014 Fabry disease is an X-linked genetic disorder characterized by deficient activity of α-galactosidase A (GLA) and accumulation of glycolipids, and various GLA gene mutations lead to a wide range of clinical phenotypes from the classic form to the later ...
Takahiro Tsukimura +7 more
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Enzyme Replacement Therapy in the Treatment of Fabry Disease
罕见病研究, 2023 Fabry disease is an X-linked inherited lysosomal storage disease caused by the mutation of GLA gene that encodes α-galactosidase A (α-Gal A). GLA gene mutation causes the decline or deficiency in the activity of α-Gal A, leading to the accumulation of ...
YU Che, WANG Rong
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Development of microbial and/or enzymatic systems for the valorization of lignocellulosic wastes [PDF]
, 2009 The future trend of white biotechnology is the seek of renewable resources for the production of goods, traditionally obtained from petrol. Shifting society’s dependence away from petroleum to renewable biomass resources is generally viewed as an ...
Iandolo, Donata
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Fabry disease, a complex pathology not easy to diagnose
Cardiogenetics, 2015 Fabry disease is a multisystemic lysosomal storage disorder, inherited in an X-linked manner. It is a defect of metabolism of the glycosphingolipids, due to the reduction or absence of the activity of lysosomal enzyme α-galactosidase A. This reduction of
Paolo Colomba +8 more
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Production of a Highly Protease-Resistant Fungal α-Galactosidase in Transgenic Maize Seeds for Simplified Feed Processing. [PDF]
PLoS ONE, 2015 Raffinose-family oligosaccharide (RFO) in soybeans is one of the major anti-nutritional factors for poultry and livestocks. α-Galactosidase is commonly supplemented into the animal feed to hydrolyze α-1,6-galactosidic bonds on the RFOs.
Wenxia Yang +9 more
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