Antigenicity of a viral peptide displayed on β-galactosidase fusion proteins is influenced by the presence of the homologous partner protein [PDF]
FEMS Microbiology Letters, 1996 Several beta-galactosidase fusion proteins have been constructed containing the entire VP1 protein from foot-and-mouth disease virus (FMDV) [Corchero et al. (1996) J. Biotechnol. in press]. The antigenicity of the major immunodominant site A (13 amino acids in length) within the VP1 protein has been studied in competitive ELISA using a panel of seven ...
J L, Corchero, A, Villaverde
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Taro α-galactosidase: A new gene product for blood conversion [PDF]
, 2012 The N-terminal sequence and internal sequences of the purified taro α-galactosidase (α-Gal) were determined using tandem mass spectrometry. By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with designed ...
Chern, Ming-kai; Chien, Su-fang +1 more
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High gene expression inEscherichia coliof recombinant alginate lyase as a fused protein with β-galactosidase α-peptide [PDF]
FEMS Microbiology Letters, 1995 Escherichia coli LE392 (pAL28) was previously isolated as a positive clone harboring the alginate lyase gene (aly) from an alginate-degrading strain, Pseudomonas sp. OS-ALG-9. The plasmid pAL205, one of the constructs obtained after successive subcloning of pAL28, gave the highest expression of aly in E. coli cells.
K, Fujiyama +3 more
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Production of secreted guar α-galactosidase by Lactococcus lactis [PDF]
, 1995 A plant α-galactosidase gene was inserted in the expression vector pGKV259. The resulting plasmid pGAL2 consisted of the replication functions of the broad-host-range lactococcal plasmid pWV01, the lactococcal promoter P59, and the DNA sequences encoding
Bolhuis, A. +9 more
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Lactobacillus fermentumCRL 722 is able to deliver active α-galactosidase activity in the small intestine of rats [PDF]
FEMS Microbiology Letters, 2005 alpha-galactooligosaccharides (alpha-GOS) found in legumes such as soybeans can cause gastrointestinal disorders since mammals lack alpha-galactosidase (alpha-Gal) in the small intestine which is necessary for their hydrolysis. Lactobacillus fermentum CRL 722 is a lactic acid bacterium (LAB) capable of degrading alpha-GOS due to its elevated alpha-Gal ...
Leblanc, Jean Guy +3 more
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Compression behavior of the enzyme ß-galactosidase [PDF]
, 2004 This thesis is based on the investigation of the compression behavior of a solid model enzyme. It was the scope of this work to characterize the behavior of the enzyme powder under pressure to gain on the one hand information about the behavior of ...
Kuny, Tanja Tamara
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Coconut α-d-galactosidase isoenzymes: isolation, purification and characterization [PDF]
, 2021 α-d-Galactosidases (α-d-galactoside galactohydrolase, EC 3.2.1.22) from normal coconut endosperm were isolated and partially purified by a combination of ammonium sulfate fractionation, SP-Sephadex C50-120 ion-exchange chromatography and Sephadex G-200 ...
Mendoza, Evelyn Mae T. +2 more
core
Insertion of a 27 amino acid viral peptide in different zones ofEscherichia coliβ-galactosidase: Effects on the enzyme activity [PDF]
FEMS Microbiology Letters, 1994 Seven internal, putatively exposed regions of Escherichia coli beta-galactosidase have been explored regarding their tolerance to insertions of large foreign peptides. Small sequence modifications, including amino acid substitutions and small deletions, were introduced into the lacZ gene to generate unique BamHI restriction sites. By using these mutant
A, Benito, A, Villaverde
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A Novel Alkaline α-Galactosidase from Melon Fruit with a Substrate Preference for Raffinose1 [PDF]
, 1999 The cucurbits translocate the galactosyl-sucrose oligosaccharides raffinose and stachyose, therefore, α-galactosidase (α-d-galactoside galactohydrolase, EC 3.2.1.22) is expected to function as the initial enzyme of photoassimilate catabolism.
Zhifang Gao, Arthur A. Schaffer
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Human Genomics, 2006 Fabry disease, an X-linked recessive inborn error of glycosphingolipid catabolism, results from the deficient activity of the lysosomal exoglycohydrolase, α-galactosidase A (EC 3.2.1.22; α-Gal A).
Shabbeer Junaid +3 more
doaj +1 more source