Results 1 to 10 of about 76,944 (297)

Genetic Fusion of an Anti-BclA Single-Domain Antibody with Beta Galactosidase [PDF]

Antibodies, 2018
The Bacillus collagen-like protein of anthracis (BclA), found in Bacillus anthracis spores, is an attractive target for immunoassays. Previously, using phage display we had selected llama-derived single-domain antibodies that bound to B.
George P. Anderson, Lisa C. Shriver-Lake, Scott A. Walper, Lauryn Ashford, Dan Zabetakis, Jinny L. Liu, Joyce C. Breger, P. Audrey Brozozog Lee, Ellen R. Goldman   +8 more
doaj   +2 more sources

Safety evaluation of the food enzyme beta‐galactosidase from Bacillus sp. (strain M3‐1) [PDF]

EFSA Journal, 2019
The food enzyme β‐d‐galactosidase galactohydrolase (EC 3.2.1.23) is produced with Bacillus sp. strain M3‐1 by GenoFocus Inc. The food enzyme β‐galactosidase is intended to be used in the manufacture of galactooligosaccharides (GOS).
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), Vittorio Silano, José Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Alicja Mortensen, Gilles Rivière, Inger‐Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Klaus‐Dieter Jany, Francesca Marcon, André Penninks, Jaime Aguilera, Magdalena Andryszkiewicz, Natália Kovalkovičová, Yi Liu, Andrew Chesson   +23 more
doaj   +2 more sources

An Optimized Protocol for Histochemical Detection of Senescence-associated Beta-galactosidase Activity in Cryopreserved Liver Tissue [PDF]

Journal of Histochemistry and Cytochemistry, 2020
Giulia Jannone, Mustapha Najimi, Etienne Sokal   +2 more
exaly   +2 more sources

Rapid Immunochromatographic Detection of Serum Anti-α-Galactosidase A Antibodies in Fabry Patients after Enzyme Replacement Therapy. [PDF]

PLoS ONE, 2015
We developed an immunochromatography-based assay for detecting antibodies against recombinant α-galactosidase A proteins in serum. The evaluation of 29 serum samples from Fabry patients, who had received enzyme replacement therapy with agalsidase alpha ...
Sachie Nakano, Takahiro Tsukimura, Tadayasu Togawa, Toya Ohashi, Masahisa Kobayashi, Katsuyoshi Takayama, Yukuharu Kobayashi, Hiroshi Abiko, Masatsugu Satou, Tohru Nakahata, David G Warnock, Hitoshi Sakuraba, Futoshi Shibasaki   +12 more
doaj   +1 more source

Cell permeabilization of Kluyveromyces and Saccharomyces species to obtain potential biocatalysts for lactose hydrolysis

Acta Scientiarum: Biological Sciences, 2022
Yeast’s beta-galactosidase is an intracellular enzyme, through which it is possible to determine in vivo its activity as a biocatalyst in the lactose hydrolysis.
Érika de Pádua Alves, Alessandra Bosso, Luiz Rodrigo Ito Morioka, Hélio Hiroshi Suguimoto   +3 more
doaj   +1 more source

Development of a Biosimilar of Agalsidase Beta for the Treatment of Fabry Disease: Preclinical Evaluation

Drugs in R&D, 2023
Background and Objective Fabry disease (FD) is a rare lysosomal storage disorder caused by a deficiency of the enzyme α-galactosidase A (aGal A). Since 2001, two different enzyme replacement therapies have been authorized, with agalsidase beta being used
André B. P. van Kuilenburg, Carla E. M. Hollak, Ana Travella, Melisa Jacobs, Lucas D. Gentilini, René Leen, Karen M. M. Ghauharali-van der Vlugt, Femke S. Beers Stet, Susan M. I. Goorden, Sanne van der Veen, Marcelo Criscuolo, Mariana Papouchado   +11 more
doaj   +1 more source

Study of β-Galactosidase Enzyme Activity Produced by Lactobacilli in Milk and Cheese [PDF]

Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2008
Background and objective Lactose intolerance is a discomfort state that occurs in some people after ingestion of milk and it is due to insufficient amount of beta galactosidase in the human gut to digest lactose.
J Nowroozi, Rahbar Roshandel, Gheytanchihandel   +2 more
doaj   +1 more source

A novel Arthrobacter beta-galactosidase with homology to eucaryotic beta-galactosidases

Journal of Bacteriology, 1997
An Arthrobacter beta-galactosidase has homology with the lysosomal acid beta-galactosidases from humans and mice and with a Xanthomonas manihotis enzyme. Phylogenetic analysis of the deduced amino acid sequence showed an unusual pattern, with this procaryotic enzyme clustering within the animal clade.
K, Gutshall, K, Wang, J E, Brenchley
openaire   +3 more sources

On the evolution of beta-galactosidase. [PDF]

Proceedings of the National Academy of Sciences, 1978
The amino acid sequence of beta-galactosidase (beta-D-galactoside galactohydrolase, EC 3.2.1.23) has been compared to itself and to other proteins. Two segments, each of about 380 amino acids, comprising the first three-fourths of the polypeptide chain, were found to be very similar to each other. It is concluded that they are homologous.
J M, Hood, A V, Fowler, I, Zabin
openaire   +2 more sources

Hydrolysis of a novel lysosomotropic enzyme substrate for beta-galactosidase within intact cells.

Journal of Lipid Research, 1994
With the goal of improving the detection of lysosomal sphingolipid hydrolases within intact cells, we have recently synthesized a new fluorophor, O-[4-(1-imidazolyl)butyl]-2,3-dicyano-1,4-hydroquinonyl beta-D-galactopyranoside (Im-DCH-beta-Gal).
C R Kaneski, S A French, M R Brescia, M J Harbour, S P Miller   +4 more
doaj   +1 more source