Hydrolysis of a novel lysosomotropic enzyme substrate for beta-galactosidase within intact cells.
Journal of Lipid Research, 1994 With the goal of improving the detection of lysosomal sphingolipid hydrolases within intact cells, we have recently synthesized a new fluorophor, O-[4-(1-imidazolyl)butyl]-2,3-dicyano-1,4-hydroquinonyl beta-D-galactopyranoside (Im-DCH-beta-Gal).
C R Kaneski +4 more
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Detection of Cellular Senescence in Human Primary Melanocytes and Malignant Melanoma Cells In Vitro
Cells, 2022 Detection and quantification of senescent cells remain difficult due to variable phenotypes and the absence of highly specific and reliable biomarkers. It is therefore widely accepted to use a combination of multiple markers and cellular characteristics ...
Tom Zimmermann +5 more
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Practical Preparation of Epilactose Produced with Cellobiose 2-Epimerase from Ruminococcus albus NE1 [PDF]
, 2010 A practical purification method for a non-digestible disaccharide, epilactose (4-O-beta-galactosyl-D-mannose), was established. Epilactose was synthesized from lactose with cellobiose 2-epimerase and purified by the following procedure: (i) removal of ...
Hamada, Shigeki +4 more
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Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord
Frontiers in Cell and Developmental Biology, 2021 Programmed cell senescence is a cellular process that seems to contribute to embryo development, in addition to cell proliferation, migration, differentiation and programmed cell death, and has been observed in evolutionary distant organisms such as ...
Jorge Antolio Domínguez-Bautista +2 more
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COVID-19 and chronological aging : senolytics and other anti-aging drugs for the treatment or prevention of corona virus infection? [PDF]
, 2020 COVID-19, also known as SARS-CoV-2, is a new emerging zoonotic corona virus of the SARS (Severe Acute Respiratory Syndrome) and the MERS (Middle East Respiratory Syndrome) family.
Auwerx +30 more
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Molecules, 2022 α-Galactosidase (EC 3.2.1.22) refers to a group of enzymes that hydrolyze oligosaccharides containing α-galactoside-banded glycosides, such as stachyose, raffinose, and verbascose.
Yan Zhao +4 more
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The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy [PDF]
, 2021 To resolve the signaling mechanisms that mediate the starvation-induced processes of Dictyostelium sporulation and encystation, we performed insertional mutagenesis on cells harboring an mRFP-tagged spore gene.
Schaap, Pauline, Yamada, Yoko
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beta-Galactosidase chimeras: primary structure of a lac repressor-beta-galactosidase protein. [PDF]
Proceedings of the National Academy of Sciences, 1978 A protein possessing both lac repressor and beta-galactosidase activities in a single polypeptide of about 155,000 daltons was purified from a deletion mutant of Escherichia coli in which the lacI and Z genes are fused. A 77-residue cyanogen bromide peptide containing the fusion joint was isolated.
A J, Brake +4 more
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DNA rearrangement activity during retinoic acid-induced neural differentiation of P19 mouse embryonal carcinoma cells. [PDF]
, 2004 Because of the many superficial similarities between the immune system and the central nervous system, it has long been speculated that somatic DNA recombination is, like the immune system, involved in brain development and function.
Kawabata, Masahiro +2 more
core +1 more source
A novel Arthrobacter beta-galactosidase with homology to eucaryotic beta-galactosidases
Journal of Bacteriology, 1997 An Arthrobacter beta-galactosidase has homology with the lysosomal acid beta-galactosidases from humans and mice and with a Xanthomonas manihotis enzyme. Phylogenetic analysis of the deduced amino acid sequence showed an unusual pattern, with this procaryotic enzyme clustering within the animal clade.
K, Gutshall, K, Wang, J E, Brenchley
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