Results 11 to 20 of about 871,979 (295)

Evaluation of beta-galactosidase activity in tissue in the presence of blood [PDF]

, 2000
The reporter gene for beta -galactosidase is frequently used to determine the efficiency of gene transfer in arteries. However, blood is often present in arterial explants and may compromise the results by the presence of hemoglobin. The light absorption
Pelisek, Jaroslav, Nikol, Sigrid, Armeanu, Sorin   +2 more
core   +1 more source

Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord

Frontiers in Cell and Developmental Biology, 2021
Programmed cell senescence is a cellular process that seems to contribute to embryo development, in addition to cell proliferation, migration, differentiation and programmed cell death, and has been observed in evolutionary distant organisms such as ...
Jorge Antolio Domínguez-Bautista, Pilar Sarah Acevo-Rodríguez, Susana Castro-Obregón   +2 more
doaj   +1 more source

Isolation and Identification of an α-Galactosidase-Producing Lactosphaera pasteurii Strain and Its Enzymatic Expression Analysis

Molecules, 2022
α-Galactosidase (EC 3.2.1.22) refers to a group of enzymes that hydrolyze oligosaccharides containing α-galactoside-banded glycosides, such as stachyose, raffinose, and verbascose.
Yan Zhao, Jinghui Zhou, Shan Dai, Xiaozhu Liu, Xuewen Zhang   +4 more
doaj   +1 more source

Determination of Cell Permeabilization and Beta-Galactosidase Extraction from Aspergillus oryzae CCT 0977 Grown in Cheese Whey

International Journal of Chemical Engineering, 2018
Aspergillus oryzae grown in cheese whey has the ability to produce beta-galactosidase. The objective of this work was to define the parameters for the determination of cell permeabilization and extraction of the enzyme from Aspergillus oryzae CCT 0977 ...
Caroline dos Santos Viana, Denise Renata Pedrinho, Luiz Rodrigo Ito Morioka, Hélio Hiroshi Suguimoto   +3 more
doaj   +1 more source

Three novel beta-galactosidase gene mutations in Han Chinese patients with GM1 gangliosidosis are correlated with disease severity

Journal of Biomedical Science, 2010
Background GM1 gangliosidosis (GM1) is an autosomal recessive lysosomal storage disease caused by deficiency of acid beta-galactosidase (GLB1; EC3.2.1.23).
Wu Jer-Yuarn, Yang Chi-Fan, Tsai Fuu-Jen
doaj   +1 more source

Biochemical Insights into a Novel Family 2 Glycoside Hydrolase with Both β-1,3-Galactosidase and β-1,4-Galactosidase Activity from the Arctic

, 2023
A novel GH2 (glycoside hydrolase family 2) β-galactosidase from Marinomonas sp. BSi20584 was successfully expressed in E. coli with a stable soluble form.
Zheng Wang, Guoqing Niu, Haitao Ding, Dianyi Li, Bo Chen, Yong Yu, Huirong Li, Wei Luo   +7 more
core   +1 more source

Enzyme activities and pectin breakdown of sapodilla submitted to 1-methylcyclopropene Atividade de enzimas e degradação de pectinas de sapoti submetido ao 1-metilciclopropeno

Pesquisa Agropecuária Brasileira, 2008
The objective of this work was to investigate the influence of 1-methylcyclopropene (1-MCP) at 300 nL L-1 on activities of cell wall hidrolytic enzymes and pectin breakdown changes which Sapodilla (Manilkara zapota cv.
Patrícia Lígia Dantas de Morais, Luiz Carlos de Oliveira Lima, Maria Raquel Alcântara de Miranda, José Donizete Alves, Ricardo Elesbão Alves, José Daniel Silva   +5 more
doaj   +1 more source

Ondas ultrassônicas e pérolas de vidro: um novo método de extração de beta-galactosidase para uso em laboratório Ultrasonic waves and glass pearls: a new method of extraction of beta-galactosodade for use in laboratory

Química Nova, 2008
The optimization of a traditional technique of cellular disruption by abrasion was carried out and a process using ultrasonic waves associated with glass pearls to extract beta-galactosidase from Kluyveromyces marxianus proposed.
Fabiana Oliveira de Medeiros, Fernanda Germano Alves, Cristiane Reinaldo Lisboa, Daniela de Souza Martins, Carlos André Veiga Burkert, Susana Juliano Kalil   +5 more
doaj   +1 more source

On the evolution of beta-galactosidase. [PDF]

Proceedings of the National Academy of Sciences, 1978
The amino acid sequence of beta-galactosidase (beta-D-galactoside galactohydrolase, EC 3.2.1.23) has been compared to itself and to other proteins. Two segments, each of about 380 amino acids, comprising the first three-fourths of the polypeptide chain, were found to be very similar to each other. It is concluded that they are homologous.
J M, Hood, A V, Fowler, I, Zabin
openaire   +2 more sources

Evaluation of p-naphtholbenzein-beta-D-galactoside as a substrate for bacterial beta-galactosidase

We describe the synthesis of a new substrate for the detection of beta -galactosidase and evaluate its performance in comparison with 5-bromo-4-chloro-3-indolyl-beta -D-galactopyranoside (X-Gal) and cyclohexenoesculetin-beta -D-galactoside (CHE-Gal).
James AL, Armstrong L, Chilvers KF, Gould FK, Perry JD   +4 more
core   +4 more sources