Results 71 to 80 of about 626,384 (268)
Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level
Aging and Cancer, EarlyView.This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley +1 more source
Expression and characterization of recombinant α-galactosidase from rice [PDF]
, 2010 碩士半乳糖水解酶可分解多醣，水解B型紅血球表面抗原的半乳糖基，使B型紅血球轉換成O型紅血球，可用於臨床輸血。本研究將稻米半乳糖水解酶基因整合到pPIC9K質體，在轉殖到重組酵母菌(Pichia pastoris)的染色體DAN上，並以4 mg/ml G418篩選出具有multicopy的菌株，以表現稻米半乳糖水解酶。重組酵母菌之培養條件測試，分別針對培養基種類、誘導溫度、誘導起始A600值、每日甲醇量及時間間隔、誘導培養基添加額外碳源檢測其對產量的影響，得到最佳表現量，細胞內酵素單位為5.124 ...
陳詩慧; Chen, Shi-hui
core
Valuation of agro-industrial wastes as substrates for heterologous production of α-galactosidase
Microbial Cell Factories, 2018 Background The recycling of agro-industrial wastes is at present limited by the availability of efficient and low-cost enzyme cocktails. The use of these materials as culture media to produce the enzymes can contribute to the profitability of the ...
María-Efigenia Álvarez-Cao +4 more
doaj +1 more source
Future Foods, 2021 Intake of Fermentable Oligo,- Di,- Monosaccharides, And Polyols, abbreviated as FODMAP, is known to worsen gastrointestinal symptoms in people with functional bowel disorders.
Antti Nyyssölä +7 more
doaj +1 more source
Senolytic Therapy as a Preventive Strategy for Spine Degeneration and Pain
Advanced Science, EarlyView.Cellular senescence promotes inflammation, tissue degeneration, and chronic back pain. In sparc‐null mice, early oral administration of the senolytic agents o‐vanillin and RG‐7112 reduced senescent cell burden and pro‐inflammatory SASP signaling across intervertebral discs, endplates, vertebral bone, and spinal cord.
Saber Ghazizadeh +7 more
wiley +1 more source
Gene cloning and expression of taro α-galactosidase [PDF]
, 2010 碩士α-半乳糖水解酶（α-galactosidase）是一種可以將末端半乳糖水解的酵素，在各種生物體中都存在，並且擁有許多不同的生物功能，例如在植物中α-半乳糖水解酶參與醣類的代謝和運輸（source to sink transport）。在應用方面，α-半乳糖水解酶可以使B型紅血球表面抗原上的半乳糖水解而變成O型紅血球，因此藉由基因工程的方法取得基因並利用 Pichia pastoris 的蛋白質表現系統來產生大量的芋頭 α-半乳糖水解酶以進行血行轉換的測試和應用 ...
李皇毅; Lee, Houng-yi
core
浙江大学学报. 农业与生命科学版, 2012 YGM-α-gal, an expression vector for Saccharomyces cerevisiae, was constructed containing α-galactosidase gene as selection marker and an inducible GAL1 promoter.
ZHANG Wei +3 more
doaj +1 more source
Advanced Science, EarlyView.Cigarette smoke accelerates the aging of immune cells in the lung. By combining human single‐cell sequencing, cell culture, and mouse models, the authors show that the protein GEM drives mitochondrial damage and senescence in alveolar macrophages by suppressing SIRT3.
Jin Wang +8 more
wiley +1 more source
Expression and characterization of recombinant α-galactosidase from taro [PDF]
, 2014 碩士α-半乳糖水解酵素（α-galactosidase）是一種可以將非還原端半乳糖水解的酵素，在各種生物體中都存在，並且擁有許多不同的生物功能，例如在植物中α-半乳糖水解酵素參與醣類的代謝和運輸。在應用方面，α-半乳糖水解酵素可以使B型紅血球表面抗原上的半乳糖水解而變成O型紅血球，因此藉由基因工程的方法取得基因並利用 Pichia pastoris 的蛋白質表現系統來產生大量的芋頭α-半乳糖水解酵素以進行血型轉換的測試和應用。 本研究將已轉殖入α-半乳糖水解酵素基因的酵母菌大量培養 ...
陳逸書; Chen, I-Su
core
Kinetics of soluble carbohydrates by action of endo/exo α-galactosidase enzyme in lentils and peas [PDF]
, 2016 The kinetics of soluble sugars (available sugars and α-galactosides) during the action of naturally occurring endogenous α-galactosidase for up to 90 min in lentil and pea flours has been studied, and it has been compared with the addition of commercial ...
Doblado, Rosa +2 more
core +1 more source