Expression and characterization of recombinant α-galactosidase from rice
, 2010 碩士半乳糖水解酶可分解多醣，水解B型紅血球表面抗原的半乳糖基，使B型紅血球轉換成O型紅血球，可用於臨床輸血。本研究將稻米半乳糖水解酶基因整合到pPIC9K質體，在轉殖到重組酵母菌(Pichia pastoris)的染色體DAN上，並以4 mg/ml G418篩選出具有multicopy的菌株，以表現稻米半乳糖水解酶。重組酵母菌之培養條件測試，分別針對培養基種類、誘導溫度、誘導起始A600值、每日甲醇量及時間間隔、誘導培養基添加額外碳源檢測其對產量的影響，得到最佳表現量，細胞內酵素單位為5.124 ...
陳詩慧; Chen, Shi-hui
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β-galactosidase-specific band shifts were not observed in monocots and basal angiosperms samples.
, 2023 (A) Tomato fruits produced GBGM oligo after AnGH5 digestion, which migrated to GAGM after the following β-galactosidase treatment. M: marker lane (standard oligo saccharides).
Alberto Echevarría-Poza (17692296) +7 more
core +1 more source
Gene cloning and expression of taro α-galactosidase
, 2010 碩士α-半乳糖水解酶（α-galactosidase）是一種可以將末端半乳糖水解的酵素，在各種生物體中都存在，並且擁有許多不同的生物功能，例如在植物中α-半乳糖水解酶參與醣類的代謝和運輸（source to sink transport）。在應用方面，α-半乳糖水解酶可以使B型紅血球表面抗原上的半乳糖水解而變成O型紅血球，因此藉由基因工程的方法取得基因並利用 Pichia pastoris 的蛋白質表現系統來產生大量的芋頭 α-半乳糖水解酶以進行血行轉換的測試和應用 ...
李皇毅; Lee, Houng-yi
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A rapid permeabilization procedure for accurate quantitative determination of β-galactosidase activity in yeast cells [PDF]
FEMS Microbiology Letters, 1995 A procedure is described which allows the rapid permeabilization of yeast cells, Schizosaccharomyces pombe and Saccharomyces cerevisiae, for quantitative in situ assays of beta-galactosidase activity. Yeast cells are permeabilized by incubation in buffer containing 0.2% of the detergent sodium lauroyl sarcosinate without any need for washing or ...
openaire +2 more sources
Hidrólise da lactose e síntese de galactooligossacarídeos utilizando B-galactosidase imobilizada em suportes à base de quitosana [PDF]
, 2014 Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia de Alimentos, Florianópolis, 2014.A ß-galactosidase (E.C 3.2.1.23) é uma das enzimas mais empregadas na indústria de alimentos sendo ...
Klein, Manuela Poletto
core
Taro α-galactosidase: A new gene product for blood conversion
, 2012 The N-terminal sequence and internal sequences of the purified taro α-galactosidase (α-Gal) were determined using tandem mass spectrometry. By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with designed ...
Chern, Ming-kai; Chien, Su-fang +1 more
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Expression and characterization of recombinant α-galactosidase from taro
, 2014 碩士α-半乳糖水解酵素（α-galactosidase）是一種可以將非還原端半乳糖水解的酵素，在各種生物體中都存在，並且擁有許多不同的生物功能，例如在植物中α-半乳糖水解酵素參與醣類的代謝和運輸。在應用方面，α-半乳糖水解酵素可以使B型紅血球表面抗原上的半乳糖水解而變成O型紅血球，因此藉由基因工程的方法取得基因並利用 Pichia pastoris 的蛋白質表現系統來產生大量的芋頭α-半乳糖水解酵素以進行血型轉換的測試和應用。 本研究將已轉殖入α-半乳糖水解酵素基因的酵母菌大量培養 ...
陳逸書; Chen, I-Su
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Low copy number vectors for expression of fused genes to β-galactosidase inEscherichia coli [PDF]
FEMS Microbiology Letters, 1991 A series of six expression vectors, pXM184Lac.A, B, C, pXM184Z.A, B, C, based on the low copy plasmid pACYC184 that allow for expression of proteins fused to beta-galactosidase in Escherichia coli is described. A level of 50,000 units of beta-galactosidase is routinely observed and is easily identifiable on protein gels.
openaire +2 more sources
Coconut α-d-galactosidase isoenzymes: isolation, purification and characterization
, 2021 α-d-Galactosidases (α-d-galactoside galactohydrolase, EC 3.2.1.22) from normal coconut endosperm were isolated and partially purified by a combination of ammonium sulfate fractionation, SP-Sephadex C50-120 ion-exchange chromatography and Sephadex G-200 ...
Mendoza, Evelyn Mae T. +2 more
core
Evaluating the Metabolic Basis of α-Gal A mRNA Therapy for Fabry Disease
BiologymRNA injection-based protein supplementation has emerged as a feasible treatment for Fabry disease. However, whether the introduction of LNP-encapsulated mRNA results in the alteration of metabolomics in an in vivo system remains largely unknown.
Zhendong Zhang +6 more
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