Results 91 to 100 of about 2,563,124 (253)

High-throughput β-galactosidase and β-glucuronidase Assays Using Fluorogenic Substrates

Bio-Protocol, 2013
β-galactosidase and β-glucuronidase enzymes are commonly used as reporters for gene expression from gene promoter-lacZ or uidA fusions (respectively). The protocol described here is a high-throughput alternative to the commonly used Miller assay (Miller,
Josh Ramsay
doaj   +1 more source

Measuring Faecal Epi-Androsterone as an Indicator of Gonadal Activity in Spotted Hyenas (Crocuta crocuta). [PDF]

PLoS ONE, 2015
Enzyme immunoassays (EIA) that measure faecal testosterone metabolites (fTM) are useful tools to monitor gonadal activity. The aim of this study was to validate an "in-house" epiandrosterone EIA to monitor fTM in spotted hyenas. FTM were characterised in
Susanne Pribbenow, Marion L East, Andre Ganswindt, Adrian S W Tordiffe, Heribert Hofer, Martin Dehnhard   +5 more
doaj   +1 more source

Amoxapine Demonstrates Incomplete Inhibition of β-Glucuronidase Activity from Human Gut Microbiota

SLAS discovery : advancing life sciences R & D, 2018
Amoxapine has been demonstrated to be a potent inhibitor of Escherichia coli β-glucuronidase. This study aims to explore the factors causing unsatisfactory efficacy of amoxapine in alleviating CPT-11–induced gastrointestinal toxicity in mice and to ...
Yang Wei, Wei Bin, Yan Ru
semanticscholar   +1 more source

CryoBN‐PAGE: A Rapid and Cost‐Effective Method for the Analysis of Yeast Respiratory Complexes and Their Dynamic Organization

Yeast, EarlyView.
We present cryoBN‐PAGE, a method that enables blue native polyacrylamide gel electrophoresis analysis of mitochondrial respiratory complexes and supercomplexes directly from whole yeast cells using cryogenic milling. This approach eliminates the need for mitochondrial isolation, reducing time and cost while preserving native protein complexes.
Lizeth J. Camacho Lopez, Mazzen H. Eldeeb, Elliana G. Mohrhardt, Flavia Fontanesi   +3 more
wiley   +1 more source

Structure-guided engineering of the substrate specificity of a fungal β-glucuronidase toward triterpenoid saponins

Journal of Biological Chemistry, 2017
Glycoside hydrolases (GHs) have attracted special attention in research aimed at modifying natural products by partial removal of sugar moieties to manipulate their solubility and efficacy.
B. Lv, Han-Li Sun, Shen Huang, Xudong Feng, T. Jiang, Chun Li   +5 more
semanticscholar   +1 more source

Prevalence of novel psychoactive substances in selected clinical urine specimens submitted for drug monitoring

Journal of Forensic Sciences, EarlyView.
Abstract Novel psychoactive substances (NPS) are compounds emerging as alternatives to more established drugs of abuse. They are constantly changing and being modified, which makes their detection difficult. The study objective was to determine the prevalence of NPS in selected clinical specimens. Deidentified urine specimen remnants were analyzed that
Jillian R. Neifeld, Theresa M. Meli, Sarah H. Bartock   +2 more
wiley   +1 more source

Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat

, 2011
The uidA gene, encoding for β-glucuronidase (GUS), is the most frequently used reporter gene in plants. As a reporter enzyme, GUS can be assayed both qualitatively and quantitatively.
M. M. Mahfouz, A. Bahieldin, A. M. Ramadan, O. M. Saleh, Hala F. Eissa, M. Salama, Bahieldin, Ahmed M., N. E. Ibrahim, Saleh, Osama Mesilhy, Ramadan, Ahmed M Ali, Mahfouz, Magdy M., Salama, M. I., Ibrahim, Nasser E., Eissa, Hala F., F. M. El-Domyati, El-Domyati, Fotouh M.   +15 more
core   +1 more source

Protodesnitazene: The identification and tissue distribution of a new nitazene

Journal of Forensic Sciences, EarlyView.
Abstract Multiple new analogues from the 2‐benzyl benzimidazole or “nitazene” class of drugs have emerged on the drug market in recent years. Nitazenes are highly potent μ‐opioid receptor agonists that present a substantial public health concern due to their elevated risk of severe toxicity in overdose and their significant potential for misuse.
Andrew Griffiths, Nathan Milne, Michelle Johnston, Karen Blakey, Rogan Scott   +4 more
wiley   +1 more source

A Chromogenic substrate for a β-xylosidase-coupled assay of α-glucuronidase

, 2000
The original publication is available at www.elsevier.com.Includes bibliography.-Nitrophenyl 2-(4-O-methyl-α- -glucopyranuronosyl)-β- -xylopyranoside obtained on deesterification of 4-nitrophenyl 2-O-(methyl 4-O-methyl-α- -glucopyranosyluronate)-β ...
Prior, Bernard A., Hirsch, Jan, Van Zyl, Willem H., Biely, Peter, La Grange, Daniel C.   +4 more
core   +1 more source

TcALS knockdown accelerates metamorphosis via insulin signaling in Tribolium castaneum

Insect Science, EarlyView.
RNAi silencing of TcALS accelerates larva‐to‐pupa metamorphosis in Tribolium castaneum. Abstract Insect metamorphosis is an evolutionary adaptation precisely regulated by nutritional and hormonal cues. The red flour beetle Tribolium castaneum, a major stored‐product pest, serves as an excellent model for studying this process.
Min Li, Jie Chen, Zi‐Ye Wang, Fei‐Feng Wang, Lu‐Lu Dong, Jun Pan, Min‐Er Li, Lin Yu, Wen Sang   +8 more
wiley   +1 more source