Results 41 to 50 of about 2,690,978 (249)
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Czech Journal of Food Sciences, 1999 Polyclonal antibodies were raised against three immunogens- a-lactalbumin (LA), -lactoglobulin A (LGA) and B (LGB). Using these antibodies the procedures of an indirect competitive enzyme immunoassay (ELISA) were constructed, optimized a nd characterized
L. Karasova, P. Rauch, L. Fukal
doaj +1 more source
The concept of the Sun as ἡγεμονικόν in the Stoa and in Manilius’Astronomica
Revista Archai, 2017 Hegemonikon in Stoic vocabulary is the technical term for the chief part or command-centre’of the soul. As we know, the Stoics considered the cosmos a living organism, and they theorised both about the human soul’s Hegemonikon and about its counterpart in the World-soul.
openaire +3 more sources
On (μ1, μ2, μ3)-Weakly Generalized Closed Sets
European Journal of Pure and Applied Mathematics, 2020 This paper defines a new generalization of closed sets in a tri-generalized topological space called (μ1, μ2, μ3)-weakly generalized closed set (or briefly (μ1,μ2, μ3)-wg closed set) which is defined as follows: A subset A of X is (μ1, μ2, μ3)-weakly generalized closed set if clμ1(intμ2(A)) ⊆U whenever A ⊆ U and U is μ3-open in X.
Breix Michael Agua, Rolando N. Paluga
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Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Determination of water-insoluble β-D-glucan in the whole-grain cereals and pseudocereals
Czech Journal of Food Sciences, 2007 Water-insoluble β-(1,3)-D-glucan (lichenan) was determined in 43 samples of various cereal (oats, barley, wheat, millet) and pseudocereal (buckwheat, amaranth) cultivars using a modified procedure with fungal α-amylase (Fermizyme P 300).
Bernadetta Hozová +3 more
doaj +1 more source
Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential
FEBS Letters, EarlyView.Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta +3 more
wiley +1 more source
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
FUNGSI DAN PEMERIKSAAN LIMFOSIT γδT (Functions and Examination of γδT lymphocytes)
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY, 2018 T lymphocytes most have TCR α and β chains. However, TCR formed from γ and δ chains determine a new subset of T lymphocytes. γδTCR specific to different types of ligands, including bacterial phosphoantigen, nonclassical MHC-I molecules and unprocessed protein.
Yulia Nadar Indrasari, Jusak Nugraha
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Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
FEBS Letters, EarlyView.Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source