Results 41 to 50 of about 3,518,946 (289)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Grup D β-laktamazlar [PDF]

Class D β-lactamases, according to the Ambler molecular classification, are serine hydrolases like class A and C enzymes. Although evolutionarily related, they share less than 20% homology with classes A and C. Most class D β-lactamases are not inhibited
Hasdemir Gökboğa, Münevver Ufuk
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Ethane-β-Sultam Modifies the Activation of the Innate Immune System Induced by Intermittent Ethanol Administration in Female Adolescent Rats [PDF]

, 2014
Intermittent ethanol abuse or ‘binge drinking’ during adolescence induces neuronal damage, which may be associated with cognitive dysfunction. To investigate the neurochemical processes involved, rats were administered either 1 g/kg or 2 g/kg ethanol in ...
Colivicchi Ma, Stefanini C, Hemmings, K., Colivicchi, M.A., Stefanini, C., Della Corte, L., Dexter, D T, De Witte, P, Colivicchi, Stefanini C, Nayak, K., de Witte, Philippe, Pitard, A., Page, M.I., Corte, Della, Pittard, A., Lallemand, f, Ward, Roberta, Ward, Roberta J., Hemmings, Karl, Hemming, Karl, Lallemand, Frederic, Page, Michael I., Dexter, D. T., Dexter, David T., Page, Michael, Nayak, K, Corte, Laura Della   +25 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

PRRX1 silencing is required for metastatic outgrowth in melanoma and is an independent prognostic of reduced survival in patients

Molecular Oncology
Paired related homeobox 1 (PRRX1) is an inducer of epithelial‐to‐mesenchymal transition (EMT) in different types of cancer cells. We detected low PRRX1 expression in nevus but increased levels in primary human melanoma and cell lines carrying the ...
Josep R. Ferreres, Antònia Vinyals, Rafael Campos‐Martin, Roderic Espín, Sebastian Podlipnik, Raquel Ramos, Esther Bertran, Cristina Carrera, Joaquim Marcoval, Josep Malvehy, Isabel Fabregat, Susana Puig, Àngels Fabra   +12 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Ruthenium porphyrin-ß-cyclodextrin complexes as supramolecular enzyme models for regioselective cleavage of carotenoids [PDF]

, 2006
All naturally occurring vitamin A derives from enzymatic oxidative cleavage of β,β-carotene or other carotenoids with pro-vitamin A activity. Two metabolic pathways of β,β-carotene to retinal (vitamin A aldehyde) have been proposed: the central cleavage ...
Wang, Hao
core   +1 more source

Antioxidant activity, β-glucan and lipid contents of oat varieties

Czech Journal of Food Sciences, 2008
The antioxidant activity, total phenolic and β-glucans contents, and the fatty acid profile of total lipids in covered (black and yellow) and naked oats were studied.
Lucia Brindzová, Milan Čertík, Peter RAPTA, Michal Zalibera, Anna Mikulajová, Mária Takácsová   +5 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source