Results 61 to 70 of about 1,000 (178)

DUET‐seq: An Open‐Source Droplet Platform for High‐Fidelity Joint Chromatin and Transcriptome Profiling Reveals Temporal Regulatory Decoupling in Single Cells

open access: yesAdvanced Science, EarlyView.
DUET‐seq is an open‐source droplet platform that jointly profiles chromatin accessibility and gene expression from the same nucleus. Dissolvable dual‐linker hydrogel beads and a one‐step intra‐droplet RT‐PCR co‐index both modalities in under 12 h at roughly $0.04 per cell.
Dong Cheng   +16 more
wiley   +1 more source

βC1 physically interacts with MPK4.

open access: yes, 2019
(A) In vitro immunocomplex kinase assays show that βC1 suppresses MPK4 activity. Different purified kinases, myelin basic protein (MBP) substrates, and [γ-32P] ATP were incubated with either GST-βC1 or GST protein.
Changjun Huang (575619)   +7 more
core   +1 more source

Characterization of sequence elements from Malvastrum yellow vein betasatellite regulating promoter activity and DNA replication

open access: yesVirology Journal, 2012
Background Many monopartite begomoviruses are associated with betasatellites, but only several promoters from which were isolated and studied. In this study, the βC1 promoter from Malvastrum yellow vein betasatellite (MYVB) was characterized and ...
Zhang Jie   +4 more
doaj   +1 more source

Dynamic Subcellular Localization, Accumulation, and Interactions of Proteins From Tomato Yellow Leaf Curl China Virus and Its Associated Betasatellite

open access: yesFrontiers in Plant Science, 2020
Geminiviruses contain the largest number of species of plant viruses, and cause devastating crop diseases worldwide. The development of resistance to these viruses will require a clear understanding of viral protein function and interactions.
Hao Li   +5 more
doaj   +1 more source

Characterization of small interfering RNAs derived from the geminivirus/betasatellite complex using deep sequencing. [PDF]

open access: yesPLoS ONE, 2011
BACKGROUND: Small RNA (sRNA)-guided RNA silencing is a critical antiviral defense mechanism employed by a variety of eukaryotic organisms. Although the induction of RNA silencing by bipartite and monopartite begomoviruses has been described in plants ...
Xiuling Yang   +6 more
doaj   +1 more source

Identification and Functional Analysis of Four RNA Silencing Suppressors in Begomovirus Croton Yellow Vein Mosaic Virus

open access: yesFrontiers in Plant Science, 2022
Croton yellow vein mosaic virus (CYVMV), a species in the genus Begomovirus, is a prolific monopartite begomovirus in the Indian sub-continent. CYVMV infects multiple crop plants to cause leaf curl disease.
Ying Zhai   +7 more
doaj   +1 more source

Blue Light Receptor PHR2‐Mediated Spectrum Antiviral Immunity

open access: yesAdvanced Science, EarlyView.
Blue light suppresses RBSDV through the blue‐light receptor ZmPHR2, which interacts with ZmISP in chloroplasts to trigger ROS burst and antiviral defense. The viral effector P6 enhances ZmPHR2–ZmISP interaction, alters their distribution, and suppresses ROS.
Runyi Chen   +17 more
wiley   +1 more source

Feasibility Assessment of a Single‐pH Connected Polishing Train for Monoclonal Antibody Purification Using Mildly Acidic Protein A Elution and an Acidic‐Window Anion Exchange Membrane

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody downstream processing often requires multiple pH adjustments between Protein A capture and polishing steps. These adjustments increase process complexity and make direct unit‐operation connection more difficult. We evaluated a connected post‐capture polishing block operated at approximately pH 5.0 using mild‐pH Protein A ...
Takaki Aiso   +5 more
wiley   +1 more source

Detection of Mitochondrial Respiratory Chain Complexes by BN‐PAGE and Its Applications

open access: yesFuture Postharvest and Food, EarlyView.
Schematic workflow for the isolation and BN‐PAGE analysis of plant mitochondrial respiratory chain complexes. ABSTRACT Blue native polyacrylamide gel electrophoresis (BN‐PAGE) is a core technique for separating and characterizing plant mitochondrial respiratory chain complexes.
Jinghan Zhang, Jinyan Li, Hongliang Zhu
wiley   +1 more source

Possible canonical CaM binding motifs in CLCuMB-βC1.

open access: yes, 2019
Possible canonical CaM binding motifs in CLCuMB-βC1.
Wajid Arshad Abbasi (8072363)   +9 more
core   +1 more source

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