Results 81 to 90 of about 1,000 (178)

Transgenic selection and underlying mechanisms in apicomplexan parasites

The FEBS Journal, EarlyView.
Transgenic selection markers have driven genetic engineering in apicomplexans, enabling precise, iterative experiments. This review discusses mechanistic details of drug selection markers, strategies for marker recycling, and practical considerations for several clinically relevant parasites.
Swaroop Peddiraju, Rakhee Lohia, Nishith Gupta   +2 more
wiley   +1 more source

Co-localization analyses of the βC1-NbNBR1 interaction complex with the markers of sub-cellular compartments, and Western blot confirmation of expression of the fused proteins in Fig 1D.

, 2021
(A) YN-βC1 and YC-NbNBR1 were co-expressed by agrobacteria-mediated infiltration with some reported markers of sub-cellular compartments [Endosome: Sec2 as a marker, chloroplast, peroxisome: SK4 as a marker, Golgi, and endoplamic reticulum (ER)] in N ...
Xueping Zhou (131154), Mingzhen Zhang (1735672), Pan Gong (4070329), Fangfang Li (1972918), Tingting Zhou (163678)   +4 more
core   +1 more source

Effectiveness of plasma‐enhanced chemical vapor film in surface treatments of resin‐matrix ceramics to preserve optical characteristics after different aging processes

Journal of Prosthodontics, EarlyView.
Abstract Purpose The aim of this study was to evaluate the surface roughness and optical characteristics of three ceramics with resin matrix for computer‐aided design and computer‐aided manufacturing, after different surface treatment protocols associated or not with the application of film deposition by plasma‐enhanced chemical vapor deposition (PECVD)
Victor Alves Nascimento, Kevin Henrique Cruz, Lorena Scaion Silva, João Pedro Justino de Oliveira de Limírio, Ana Beatriz de Souza Albergardi, Elidiane Cipriano Rangel, Aldiéris Alves Pesqueira   +6 more
wiley   +1 more source

Cross-protection mediated by a βC1 deletion DNAβ associated with Tomato yellow leaf curl China virus

浙江大学学报. 农业与生命科学版, 2006
Pre-inoculation of a βC1 deletion DNAβ (Y10ΔC1β) associated with Tomato yellow leaf curl China virus (TYLCCNV) Y10 isolate (Y10) with cognate virus could mediate cross-protection to challenge-infection of cognate or heterogenous DNAβ.
YE Jian, QING Ling, ZHOU Xue-ping
doaj   +1 more source

Evaluation of the CRISPR/Cas9 system for the development of resistance against Cotton leaf curl virus in model plants

Plant Protection Science, 2020
Over the last decade, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) CRISPR/Cas9 system has been used by biologists in various fields.
Sehrish Khan, Muhammad Shahid Mahmood, Sajjad ur Rahman, Farzana Rizvi, Aftab Ahmad   +4 more
doaj   +1 more source

DNA‐Barcoded Polysaccharide Specific Monoclonal Antibodies Facilitate Sensitive and Multiplexed Detection of Cell Wall Polymers

Plant Biotechnology Journal, EarlyView.
ABSTRACT Plant cell walls are polysaccharide‐rich extracellular matrices composed of multiple complex carbohydrate polymer networks, including cellulose, hemicelluloses, pectins, and glycosylated proteins. Polysaccharide deposition critically impacts cell wall structure, and structural microheterogeneity within cell wall glycans also influences polymer
C. Frankie Griffith, Michael G. Hahn, Ian S. Wallace   +2 more
wiley   +1 more source

Nbrgs-CaM is genetically required for the functions of βC1 in PTGS suppression and symptom modulation.

, 2014
(A) GFP fluorescence in leaves of wt and 35S:dsCaM Nicotiana benthamiana plants co-infiltrated with 35S:GFP and 35S:FP, together with indicated suppressors. Photographs were taken under UV light at 3 and 5 dpi.
Changjun Huang (575619), Xueping Zhou (131154), Fangfang Li (1972918), Zhenghe Li (238151)   +3 more
core   +1 more source

Diversity in Betasatellites Associated with Cotton Leaf Curl Disease During Source-To-Sink Movement Through a Resistant Host

The Plant Pathology Journal, 2016
Cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. The disease symptoms are induced by DNA satellite known as Cotton leaf curl Multan betasatellite (CLCuMuB), dominant betasatellite in cotton but
Iftikhar Ali Khan, Khalid Pervaiz Akhtar, Fazal Akbar, Ishtiaq Hassan, Imran Amin, Muhammad Saeed, Shahid Mansoor   +6 more
doaj   +1 more source

Improved Autotetraploid Induction Methods in Four Diploid Wild Peanut Species: Arachis duranensis, A. stenosperma, A. magna, and A. batizocoi

Plant Breeding, EarlyView.
ABSTRACT Peanut (Arachis hypogaea L., 2n = 4x = 40, AABB) is an important legume crop worldwide. Wild peanuts (Arachis spp.) represent a valuable secondary gene pool with diverse disease resistances. However, most wild peanut species are diploid (2n = 2x = 20) and this ploidy barrier is a major obstacle to hybridization and gene introgression.
Chia‐Hung Shih, Jose M. Falconi Villalobos, Ryan J. Andres, Jeffrey C. Dunne, Hsuan Chen   +4 more
wiley   +1 more source

Image_1_In silico Prediction and Validations of Domains Involved in Gossypium hirsutum SnRK1 Protein Interaction With Cotton Leaf Curl Multan Betasatellite Encoded βC1.TIF

, 2019
Cotton leaf curl disease (CLCuD) caused by viruses of genus Begomovirus is a major constraint to cotton (Gossypium hirsutum) production in many cotton-growing regions of the world.
Diwaker Tripathi (703708), Muhammad Zuhaib Khan (6740408), Shahid Mansoor (219615), Imran Amin (61194), Hira Kamal (6740399), Roma Mustafa (6740405), Hanu R. Pappu (6740411), Fayyaz-ul-Amir Afsar Minhas (6740402), Muhammad Farooq (186443), Muhammad Hamza (798822)   +9 more
core   +1 more source