Results 21 to 30 of about 10,450 (119)
Constructing Serrated Boride Composite Layer for Enhancing Wear Resistance of Ti6Al4V Alloy
ABSTRACT Appropriate configuration combined with strong interface bonding is the essential requirement for developing high wear‐resistant coatings. Herein, based on the powder‐pack boriding process, the properties of Ti6Al4V alloy are effectively improved by constructing a serrate tribological boride composite layer.
Ying Wu +9 more
wiley +1 more source
ABSTRACT Tungsten is inherently brittle and particularly prone to thermal stress concentration and crack formation during additive manufacturing processes such as laser cladding, which severely limits its engineering applications as a coating material. To address this issue, this study proposes an in situ toughening strategy involving the introduction ...
Guo‐Xing Chen +12 more
wiley +1 more source
Background – Sporotrichosis is a chronic, mycotic infection caused by fungi of the genus Sporothrix. Zoonotic sporotrichosis occurs mainly through S. brasiliensis transmission, resulting from the organism's traumatic introduction via scratches or bites, or contact with exudate from contaminated cats.
Steffanie Amadei +6 more
wiley +1 more source
Urban agriculture is in an intermediate state between traditional urban green spaces and conventional rural farms in terms of environmental impacts, so that its contribution to urban sustainability requires complementarity with other green spaces. Abstract As cities expand, they encroach on agricultural land, impacting food production and natural ...
Zeyu Li +8 more
wiley +1 more source
Graphical abstract AbstractLightweight, high‐strength, and heat‐resistant protective structures have consistently been crucial for applications in extreme environments, such as aerospace, semiconductors, and nuclear power industries. Multilayered TC4/TB8 titanium (Ti) laminates, inspired by the heterostructures of natural biological shells, were ...
Tian‐Le Li +7 more
wiley +1 more source
摘 要 建立泌尿生殖系统沙眼衣原体感染阳性标本直接基因分型方法。先用敏感的扩增沙眼衣原体特异质粒 的引物扩增500份临床标本, 初筛选出100份阳性标本再用扩增沙眼衣原体主要外膜蛋白( MOM P)基因的引物扩 增, 并用嵌套式PC R使阳性标本能扩增出MOMP基因, 对获得的MOMP基因用限制性酶切片段长度多态性 ( RFLP)方法进行基因分型,并用银染观察结果。其中E型比例最高,占47% ; F型为16% ; Da、G型均为6% ; Ba、D 型各占5% ; H型及K型均为1% ,混合型K /
汪玎妍 郭辉玉 罗宪玲 蒋文玲 李质怀
doaj
Effectiveness of protected areas in the Caucasus Mountains in preventing rangeland degradation
Abstract As land use intensifies globally, it increasingly exerts pressure on protected areas. Despite open, nonforested landscapes comprising up to 40% of protected areas globally, assessments have predominately focused on forests, overlooking the major pressures on rangelands from livestock overgrazing and land conversion.
Arash Ghoddousi +12 more
wiley +1 more source
【目的】研究成人骨髓间质干细胞(MSC)基本生物学特性。【方法】采用Ficoll-Paque 淋巴细胞分离液分离成人 MSC , 体外扩增, 比较不同培养基和血清含量对MSC 生长的影响, 流式细胞仪检测MSC 表面抗原表达。【结果】成人骨髓间 质干细胞在体外扩增原代可获得(5 ~ 6)×105 个细胞, 15 代可获得(3~ 4)×1012个细胞, 随着传代次数的增加, 细胞的增殖能 力下降。L-DMEM 培养基有利于细胞的培养扩增。细胞流式细胞仪检测结果显示CD29、CD44 、CD59 ...
张丽蓉, 李 艳
doaj
Abstract Emerging infectious diseases are increasingly recognized as a significant threat to global biodiversity conservation. Elucidating the relationship between pathogens and the host microbiome could lead to novel approaches for mitigating disease impacts.
Alexander S. Romer +25 more
wiley +1 more source
目的: 检测细胞培养中支原体的污染, 建立支原体通用的 PCR 方法。方法: 根据已出版的序列设计并合成一对 支原体(柔膜体纲) 16S rRNA 基因组特异引物。对实验室所存的12 种支原体、大肠杆菌、变形杆菌及无支原体污染的组织培养 细胞进行了 PCR 扩增。以 PCR 与分离培养比较检测了 33份细胞培养标本。结果: 12种支原体均出现了约 620 bp左右的特异 性扩增带,敏感性为 100~ 1 000个支原体细胞, 且常见的 6种细胞培养污染支原体的扩增片段均能被 H indⅢ切成 280 与
赖小敏 方国源 李彩霞 王传恩
doaj

