Results 21 to 30 of about 9,946 (171)
Harnessing social media data to track species range shifts
Conservation Biology, EarlyView.Abstract Biodiversity monitoring programs and citizen science data remain heavily biased toward the Global North. Especially in megadiverse countries with limited biodiversity records, incorporating social media data can help address existing data gaps.
Shawan Chowdhury +15 more
wiley +1 more source
Cloning, Expression and Immunolocalization of Aspartyl Protease Inhibitor Gene of Angiostrongylus cantonensis [PDF]
, 2016 广州管圆线虫（Angiostrongyluscantonensis）是一种人畜共患自然疫源性寄生虫，能引起人的嗜酸性粒细胞增多性脑膜炎，也被称为广州管圆线虫病。其中间宿主是软体动物，例如福寿螺、玛瑙螺等，并以啮齿类动物等作为其终宿主。人类是广州管圆线虫的偶然宿主，因误食寄生有广州管圆线虫的第三期幼虫（L3）的福寿螺等而引起广州管圆线虫病。 寄生虫的天冬氨酸蛋白酶抑制剂（aspartylproteaseinhibitor，API）在寄生虫的寄生生活中起着重要的作用，包括寄生虫的营养摄取，组织入侵，免疫逃避，
曹斌斌
core
Constructing Serrated Boride Composite Layer for Enhancing Wear Resistance of Ti6Al4V Alloy
Rare Metals, Volume 45, Issue 1, January 2026.ABSTRACT Appropriate configuration combined with strong interface bonding is the essential requirement for developing high wear‐resistant coatings. Herein, based on the powder‐pack boriding process, the properties of Ti6Al4V alloy are effectively improved by constructing a serrate tribological boride composite layer.
Ying Wu +9 more
wiley +1 more source
Rare Metals, Volume 45, Issue 1, January 2026.ABSTRACT Tungsten is inherently brittle and particularly prone to thermal stress concentration and crack formation during additive manufacturing processes such as laser cladding, which severely limits its engineering applications as a coating material. To address this issue, this study proposes an in situ toughening strategy involving the introduction ...
Guo‐Xing Chen +12 more
wiley +1 more source
人体外周血CD3+CD4-CD8-双阴性T细胞体外扩增的实验研究
Zhongguo shiyan zhenduanxue, 2017 目的探讨人体外周血CD3+CD4-CD8-双阴性T细胞（DNT细胞）在体外进行分离和扩增的方法。方法取健康的成人外周血20ml,应用Rosettesep抗体吸附法去除CD4+T细胞和CD8+T细胞;再将DNT细胞放入anti-CD3mAb包被的培养板中,并加入rhIL-2、rhIL-4,共同培养,第10天和第14天计数细胞扩增倍数及记录扩增曲线;利用Easysep免疫磁珠法纯化,采用流式细胞仪检测其纯度。结果经分选、纯化后的DNT细胞,纯度可达94%以上;体外培养第10天和第14天 ...
王新梅, 王雪野, 韩梅, 肖中平
doaj
, 2006 本系列研究筛选了弓形虫（Toxoplasma）速殖子表膜主要抗原（SAG1或P30）及分泌排泄抗原棒状体蛋白基因（ROPl），并将二者拼接构建复合基因（sAG1／ROP1），对其进行扩增、克隆、表达，制备真核表达质粒，接种小鼠，研究其免疫保护效应 ...
周永安 +4 more
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Veterinary Dermatology, Volume 36, Issue 4, Page 474-484, August 2025.Background – Sporotrichosis is a chronic, mycotic infection caused by fungi of the genus Sporothrix. Zoonotic sporotrichosis occurs mainly through S. brasiliensis transmission, resulting from the organism's traumatic introduction via scratches or bites, or contact with exudate from contaminated cats.
Steffanie Amadei +6 more
wiley +1 more source
表达降钙素基因相关肽的单纯疱疹病毒Ⅰ型扩增子载体免疫效果观察
Zhongguo shiyan zhenduanxue, 2006 目的探讨重组pUPO-Δγ134.5-CGRP扩增子对免疫系统的作用。方法用重组扩增子感染BALB/C小鼠,分离血清及淋巴细胞,流式细胞仪法、免疫组化法、LDH法、分别检测外周血CD4、CD8分类、TCR、NK细胞、CTL细胞以及LAK细胞活性杀伤活性。结果重组扩增子明显增强了机体的免疫力。结论该重组扩增子具有增强机体免疫力的作用。
吴军 +4 more
doaj
The study of angiopoient-1 in repair inflammatory injury of Endothelial progenitor cells [PDF]
, 2010 目的：体外研究携带血管生成素-1(angiopoient-1，Ang-1)基因的慢病毒载体感染内皮祖细胞(endothelialprogenitorcells，EPCs)的方法。检测Ang-1修饰的EPCs诱导炎症后细胞间黏附因子1(intercellularadhesionmolecule-1，ICAM-1)、血管细胞黏附因子1(vascularcelladhesionmolecule-1，VCAM-1)的表达水平，评价Ang-1对炎症损伤后EPCs的修复作用。 方法：体外分离 ...
宋晶金
core
Ecological Solutions and Evidence, Volume 6, Issue 3, July–September 2025.Urban agriculture is in an intermediate state between traditional urban green spaces and conventional rural farms in terms of environmental impacts, so that its contribution to urban sustainability requires complementarity with other green spaces. Abstract As cities expand, they encroach on agricultural land, impacting food production and natural ...
Zeyu Li +8 more
wiley +1 more source