Results 31 to 40 of about 9,946 (171)
Zhongguo shiyan zhenduanxue, 2014 目的构建Tet-on调控的人HOXB4慢病毒载体,为体外扩增造血干细胞并保持其干细胞特性提供实验材料基础。方法应用PCR技术从质粒TAT-HA-HOXB4-Full中扩增编码人HOXB4的cDNA,用限制性内切酶EcoRI分别酶切HOXB4基因片段和带有Tet-on开关的可调控慢病毒载体,经T4DNA连接酶连接获得慢病毒载体Teto-Fuw-hHOXB4。对构建的HOXB4慢病毒载体进行酶切片段凝胶电泳及DNA测序分析。结果酶切片段电泳分析和DNA测序分析证明Tet ...
李秀英 +5 more
doaj
Rare Metals, Volume 44, Issue 7, Page 5045-5060, July 2025.Graphical abstract AbstractLightweight, high‐strength, and heat‐resistant protective structures have consistently been crucial for applications in extreme environments, such as aerospace, semiconductors, and nuclear power industries. Multilayered TC4/TB8 titanium (Ti) laminates, inspired by the heterostructures of natural biological shells, were ...
Tian‐Le Li +7 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2009 目的建立一种分离纯化,培养扩增人骨髓MSCs的方法,并探讨体外诱导骨髓间充质干细胞分化为脂肪细胞。方法密度梯度离心,贴壁培养和消化时间控制相结合,分离纯化人骨髓间充质干细胞,并用马血清诱导分化为脂肪细胞。结果MSCs原代培养呈均匀分布的集落样生长,呈梭形,细胞传代稳定,在体外连续传代培养9代,未发生形态学改变,无衰老征象;传代培养MSCs（P3）在20%马血清的L-DMEM培养液中分化为脂肪细胞。结论骨髓MSCs体外增殖和传代能力强,通过离体培养可使体内环境下低丰度的MSCs实现数量扩增 ...
王莹, 罗速
doaj
The Effect of Gold Nanoparticles on Polymerase Chain Reaction and The Mechanism Research [PDF]
, 2008 聚合酶链式反应（PolymeraseChainReaction，PCR）是生物医学领域最常用、最重要的技术之一，它能够实现DNA片段的体外扩增，可以满足后续的分析测试需要，因此是许多临床检测和生物分析的基础。纳米技术的发展以及与生物技术的融合，为解决生物技术中的问题提供了机遇，纳米物质对于PCR反应的影响也日益受到人们的关注。本论文研究了纳米金对PCR反应的影响，并对其影响PCR反应的机制进行了探讨。实验结果表明： 1.往PCR体系中加入一定量的纳米金（0~1.2nM）之后，PCR反应被抑制 ...
李辉
core
Effectiveness of protected areas in the Caucasus Mountains in preventing rangeland degradation
Conservation Biology, Volume 39, Issue 3, June 2025.Abstract As land use intensifies globally, it increasingly exerts pressure on protected areas. Despite open, nonforested landscapes comprising up to 40% of protected areas globally, assessments have predominately focused on forests, overlooking the major pressures on rangelands from livestock overgrazing and land conversion.
Arash Ghoddousi +12 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2012 目的对孕中期羊水标本进行体外分离、培养、扩增,研究孕中期羊水来源干细胞（AFC）的生长特性、细胞表面标记。方法采用贴壁法体外分离孕中期羊水来源干细胞,绘制细胞生长曲线;流式细胞仪检测细胞表面标记。结果原代培养细胞生长潜伏期4-6d,对数增殖期7-13d,生长平台期14-18d;传代培养细胞生长潜伏期1-2d,对数增长期3-6d,生长平台期7-10d。AFCs均一表达CD44,不表达CD45。结论从人孕中期羊水中成功分离具有干细胞性质的细胞群,在体外可迅速扩增。
常颖 +4 more
doaj
Conservation Biology, Volume 39, Issue 2, April 2025.Abstract Emerging infectious diseases are increasingly recognized as a significant threat to global biodiversity conservation. Elucidating the relationship between pathogens and the host microbiome could lead to novel approaches for mitigating disease impacts.
Alexander S. Romer +25 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】对Hela 细胞端粒酶进行活性重组并探讨其重组特异性。【方法】以RT-PCR 法从纯化的人端粒酶复合 体中扩增人类端粒酶RNA(hTR)基因片段, 将其克隆至含有SP6 启动子的pGEM3f +质粒中, 构建hTR 的体外转录体系以获 得体外合成的hTR。与端粒酶蛋白组分进行活性重组。四膜虫端粒酶RNA 及16S rRNA 作为重组反应对照组, 以探测其重 组特异性。【结果】经RT-PCR 扩增出450 bp 片段;体外转录重组载体经酶切图谱和PCR 扩增鉴定构建成功;体外转录的 ...
周俊宜, 罗超权
doaj
Ex vivo differentiation of amniotic membrane stromal cells into neural cells using tissue culture method [PDF]
, 2012 目的体外组织培养法诱导羊膜基质细胞向神经细胞分化。 方法将新鲜去上皮羊膜（dAM-0W）和完整羊膜（iAM-0W）固定于特制的培养插件(insert)上进行组织培养，采用神经细胞分化培养基诱导羊膜基质细胞的分化。在培养1周、4周时进行Hoechst染色，观察基质细胞的增殖情况。取新鲜羊膜（dAM-0W）、培养4周的去上皮羊膜（dAM-4W）及完整羊膜（iAM-4W），分别提取RNA进行实时荧光定量PCR检测、制备组织块进行免疫荧光染色、提取蛋白质进行WesternBlot印迹检测 ...
肖华强
core
Integrative Conservation, Volume 3, Issue 4, Page 312-329, December 2024.Biosphere reserves aim to protect global biodiversity alongside social and economic development. In the Cape Floristic Region, biosphere reserves are an effective conservation approach to protect arthropods against habitat transformation in this biodiverse region.
Michael J. Samways +3 more
wiley +1 more source