Results 21 to 30 of about 52,960 (211)
Zhongshan Daxue xuebao. Yixue kexue ban, 2020 目的本研究旨在研究骨肉瘤细胞来源外泌体对靶器官微环境的调控作用及其分子机制。方法提取骨肉瘤细胞143B外泌体并鉴定，通过PKH26荧光染色后经尾静脉注入裸鼠体内，24 h后提取肝、脾、肺、肾及脑组织，荧光显微镜下计数不同视野红色荧光数量；通过免疫荧光检测不同类型细胞中外泌体摄取情况；143B外泌体与人肺成纤维细胞共培养，荧光显微镜检测摄取情况，进一步利用qPCR检测炎性因子IL-1β、IL-6及TNF-α表达水平，通过蛋白印迹法检测炎性信号通路NF-κB通路p-p65和MAPK通路p-ERK及p ...
谢显彪 +7 more
doaj
Studies on the Tissue Culture of the Seminal Vesicle I. Fundamental Experiments [PDF]
, 1960 Influence of male sex hormone, female sex hormone, and adrenocortical hormoneupon the growth of the tissue of the seminal vesicle in vitro were studied by meansof the tissue culture of seminal visicle of guinea-pigs. The results were as follows.1) In the
中野, 順道
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Conservation Biology, EarlyView.Abstract Prioritization in conservation is crucial for the development of efficient and effective decision‐making policies. For many decades, the importance of some species and their habitats has been assessed and applied in conservation legislation, but bats and their diurnal roosts have ofbeen overlooked.
David López‐Bosch +6 more
wiley +1 more source
Gaoya dianqi, 2017 针对变电设备机构箱在亚热带海洋气候条件下易生锈、腐蚀、老化等严重影响变电设备安全稳定运行的问题,采用宏观观察、金属涂层厚度测试仪、附着力测试仪、扫描电镜、能谱、红外光谱和电化学阻抗谱等方法对国产和进口箱体外壳进行了对比分析,提出适合南方特殊气候条件的变电设备机构箱改进建议。试验结果表明:进口箱体外壳涂层厚度相对均匀,且涂层附着力优于国产机构箱;国产外壳基材选用碳钢,进口外壳基材选用耐腐蚀性能更好的铝合金,且进口箱体外壳基材的阻抗(Zimported substrate)是国产箱体外壳基材阻抗 ...
顾温国 +3 more
doaj
A link between increased temperature and avian body condition in a logged tropical forest
Conservation Biology, EarlyView.Abstract The combined effects of anthropogenic disturbances, such as logging and climate change, remain poorly understood; yet, they are the main threats to tropical biodiversity. Most tropical African countries lack long‐term climate data, so climate impacts on biodiversity cannot be assessed.
Moreen Uwimbabazi +7 more
wiley +1 more source
Dianci bileiqi, 2018 介绍了电力系统外绝缘复合伞套用有机硅与液体硅橡胶,针对液体硅橡胶复合外套材料的老化现象,分析了液体硅橡胶材料的老化机理及主要影响因素,阐述了复合外套材料的老化对换流站设备的影响。研究了液体硅橡胶复合外套老化修复策略,结合现场实例应用表明,液体硅橡胶复合外套材料的老化只要及时跟踪发现,选择高性能修复材料,采用合理化的工艺可进行换流站内现场修复,恢复复合外套性能,延长设备的使用寿命。高性能氟硅橡胶涂层材料是液体硅橡胶复合外套老化修复的可靠材料,重新赋予复合外套优异的憎水性和更强的耐老化性能。
韦晓星 +3 more
doaj
Effects of political identity activation and inaccurate metaperceptions on attitudes toward wolves
Conservation Biology, EarlyView.Abstract Polarization between groups can undermine durable conservation outcomes. Activating group identities (i.e., an individual's sense of self derived from membership in a group) can exacerbate differences, especially when people hold inaccurate perceptions of their peers and rivals.
Alexander L. Metcalf, Justin W. Angle
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2003 目的 制备人TRAIL配体胞外区基因工程蛋白。方法 应用RT PCR技术从激活的人外周血淋巴细胞总RNA中扩增TRAIL配体胞外区cDNA ,克隆入PCR2 .1 载体 ,测序验证后用基因重组法构建了TRAIL配体胞外区和组氨酸盒的原核表达质粒pQE hTRAIL。将重组质粒转入大肠杆菌M15中表达 ,经 1mmol LIPTG在 37℃条件下诱导 4h ,亲和层析柱纯化。用SDS -PAGE电泳和Westernblot鉴定表达产物。结果 所表达融合蛋白为人TRAIL配体胞外区分子 ,分子量 30kD ...
路丽明 +8 more
doaj
Conservation Biology, EarlyView.Abstract Despite the crucial role of human impacts on biodiversity loss, many assessments of this loss focus on single metrics, such as species richness, and overlook the multidimensional effects of human activities. Because of its importance to ecosystem functioning, we investigated the functional diversity of medium‐ and large‐sized mammal ...
Xueyou Li +8 more
wiley +1 more source
浙江大学学报. 农业与生命科学版In recent years, in vitro embryo production technology for mammals has developed rapidly, providing solutions for the preservation and efficient utilization of mammalian genetic resources with superior traits and local characteristics.
WANG Yuqing(王雨晴) +3 more
doaj +1 more source