Results 31 to 40 of about 49,529 (252)
Zhongguo shiyan zhenduanxue, 2003 目的 制备人TRAIL配体胞外区基因工程蛋白。方法 应用RT PCR技术从激活的人外周血淋巴细胞总RNA中扩增TRAIL配体胞外区cDNA ,克隆入PCR2 .1 载体 ,测序验证后用基因重组法构建了TRAIL配体胞外区和组氨酸盒的原核表达质粒pQE hTRAIL。将重组质粒转入大肠杆菌M15中表达 ,经 1mmol LIPTG在 37℃条件下诱导 4h ,亲和层析柱纯化。用SDS -PAGE电泳和Westernblot鉴定表达产物。结果 所表达融合蛋白为人TRAIL配体胞外区分子 ,分子量 30kD ...
路丽明 +8 more
doaj
Zhongguo shiyan zhenduanxue, 2022 目的 探索外泌体来源的miR-21作为胃癌奥沙利铂化疗过程中评价药物敏感性生物标志物的价值。方法透射电镜观察外泌体的形态；构建SGC7901奥沙利铂耐药细胞株，并利用RT-qPCR检测耐药细胞和亲本细胞中miR-21的表达水平；CCK8检测miR-21对SGC7901耐药细胞株增殖抑制率的影响；Western blot检测外泌体的标记蛋白；使用受试者工作特征曲线（ROC）分析奥沙利铂治疗胃癌的过程中miR-21评估药物敏感性的价值。结果miR-21过表达后，亲本细胞对奥沙利铂的敏感性由IC50 16 ...
杜沛, 李婷, 文雯
doaj
Rare Metals, EarlyView.ABSTRACT SnTe is considered a potential alternative to PbTe for thermoelectric applications. However, excessive carrier concentration and thermal conductivity result in poor thermoelectric performance. Herein, p‐type SnTe‐based materials Sn0.8‐xMn0.1Pb0.1SbxTe were synthesized, and the combined effects of Sb, Mn, and Pb were systematically investigated.
Jun‐Xiang Zhang +10 more
wiley +1 more source
浙江大学学报. 农业与生命科学版In recent years, in vitro embryo production technology for mammals has developed rapidly, providing solutions for the preservation and efficient utilization of mammalian genetic resources with superior traits and local characteristics.
WANG Yuqing(王雨晴) +3 more
doaj +1 more source
Studies on the Tissue Culture of the Seminal Vesicle I. Fundamental Experiments [PDF]
, 1960 Influence of male sex hormone, female sex hormone, and adrenocortical hormoneupon the growth of the tissue of the seminal vesicle in vitro were studied by meansof the tissue culture of seminal visicle of guinea-pigs. The results were as follows.1) In the
中野, 順道
core
Novel Non‐Equimolar High‐Entropy Zirconates With Superior Thermophysical Properties
Rare Metals, EarlyView.ABSTRACT Rare‐earth zirconates (RE2Zr2O7) are promising candidates for topcoat materials in thermal barrier coatings (TBCs). However, their practical application in TBCs is limited by poor fracture toughness and low thermal expansion coefficient (TEC).
Taoyi Lu +7 more
wiley +1 more source
Zhejiang Daxue xuebao. Lixue ban, 2010 用豆油作为氧载体加入发酵液中形成双液相发酵体系,以改善灵芝胞外多糖的发酵状况.实验表明：常见的氧载体材料在双液相发酵过程中不为灵芝菌体所利用,仅起到促进溶氧的效果；在灵芝胞外多糖的双液相发酵体系中, KLa值明显提高,溶氧水平(DO)有显著改善,发酵液的运动黏度µ有所降低,有助于降低搅拌能耗；氧载体改善了质量传递,提高了细胞的代谢水平,使胞外多糖的产量提高了44%,发酵周期缩短了约17%.说明双液相发酵体系对灵芝胞外多糖发酵是一种新型有效的途径.
LIWei-qi(李卫旗) +4 more
doaj +1 more source
Rare Metals, EarlyView.ABSTRACT Organic‐inorganic hybrid perovskites (OIHPs) with white‐light emission have significant potential in solid‐state lighting and display due to the designable structure and excellent luminescent properties. However, OIHPs are prone to damage during bending due to the brittleness of crystals, which limits their application in the field of flexible
Jinyu Xu +8 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2020 体外膜肺氧合技术(Extracorporeal Membrane Oxygenation,ECMO),作为一种呼吸和心脏功能的支持技术,出现于1970s[1]。由传统的心肺转流(cardiopulmonary bypass,CPB)技术发展而来。ECMO是体外生命支持系统的其中一种表现形式,它的基础是体外循环,却和体外循环相比具有很多优势,它的功能不止局限于循环支持,还可以支持呼吸衰竭患者的呼吸功能 ...
赖冰洁 +4 more
doaj