CSF1R通过激活PI3K/AKT信号通路促进食管癌细胞增殖、迁移与侵袭的研究
目的探讨集落刺激因子受体-1对食管癌细胞增殖、迁移与侵袭的影响及其潜在机制。方法在TE-1和KYSE-410食管癌细胞中,通过慢病毒技术分别构建CSF1R过表达与敲低稳定细胞系。采用CCK-8和克隆形成实验检测细胞增殖能力;划痕实验和Transwell实验评估细胞迁移与侵袭能力;Western Blot分析PI3K/AKT信号通路关键蛋白的表达及磷酸化水平。结果CSF1R在食管癌细胞系中相对高表达。KYSE410和TE-1中CSF1R敲低后,与对照组相比,实验组食管癌细胞的增殖能力减弱较显著 ...
苏 比努尔·阿不拉克 +5 more
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