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慢性吗啡诱导HEK293细胞GIRK1和GIRK2膜表达下调

Zhongshan Daxue xuebao. Yixue kexue ban
目的利用人胚胎肾细胞293（HEK293）观察G蛋白门控内向整流钾离子通道（GIRK）亚单位GIRK1和GIRK2在吗啡耐受后的表达和分布变化。方法使用HEK293细胞转染慢病毒pLV-CMV-GIRK1-T2A-GIRK2-P2A-MOR，构建过表达GIRK1、GIRK2和μ阿片受体（MOR）的稳转HEK293细胞，使用吗啡（1 μmol/L，24 h）处理该稳转细胞，构建吗啡耐受HEK293细胞模型。细胞免疫荧光检测细胞模型中MOR与GIRK1和GIRK2的分布及共定位情况 ...
刘冰柯, 崔宇, 韩雪, 肖力
doaj  

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Zhang X, Liu S, Pan J.
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Liu ZH, He XM, Xu KX, Yu HB, Chen J.
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Zhejiang Da Xue Xue Bao Yi Xue Ban
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Se Pu
Liu L, Xiao XH, Fang JH, Li GK.
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Zhong Nan Da Xue Xue Bao Yi Xue Ban
Yu C, Zeng W, Meekrathok P, Bu Y, Wang J, Qiu J.   +5 more
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[Role and mechanism of Wnt9a in human and mouse chronic wound healing]. [PDF]

Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi
Jia YH, Yuan YX, Hu DH, Guan H.
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[Expression of the melanoma 2-mediated pyroptosis pathway in peripheral blood mononuclear cells of patients with idiopathic inflammatory myopathies]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Chu J, Li P, Tian J, Fu D, Guo L, Sun R, Li Y.   +6 more
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