Results 111 to 120 of about 98,699 (173)

基于ＤＥＮＶ－ｐｒＭ／Ｅ基因构建ＤＥＮＶ－ＶＬＰ及其免疫学特性

Zhongshan Daxue xuebao. Yixue kexue ban, 2014
摘 要：【目的】 构建和表达登革病毒４型病毒样颗粒，初步研究其免疫学特性。【方法】 用ＲＴ－ＰＣＲ法扩增ＤＥＮＶ４－ｐｒＭ／Ｅ基因，构建重组质粒ｐＧＡＰＺ－ｓｐｒＭ／Ｅ－Ｄ４，转化Ｘ３３酵母菌株，ＳＤＳ－ＰＡＧＥ、ＷｅｓｔｅｒｎＢｌｏｔ鉴定目的蛋白表达。切片电镜检测病毒样颗粒，蔗糖密度梯度离心纯化。免疫三组Ｂａｌｂ／Ｃ小鼠，每组１５只。采用间接ＥＬＩＳＡ检测抗原特异性抗体，间接免疫荧光法检测免疫血清与天然病毒粒子的结合，流式细胞术分析小鼠脾淋巴细胞表型，酶联免疫斑点法（ＥＬＩＳＰＯＴ）检测脾分泌ＩＦＮ ...
doaj  

[Role of Trained Immunity in the Association Between Periodontitis and Rheumatoid Arthritis]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
You R, Cui Y, Sun J.
europepmc   +1 more source

免疫学 [PDF]

, 2003
岸 裕幸, 廣田 早苗, 村口 篤, 金 哲雄, 金田 尚, 韋 星呈   +5 more
core   +1 more source

[Characterization of Immune Reconstitution Patterns in B-cell Subsets Mediated by B-cell Targeted Therapies]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Wang X, Zhang Y, Duan Z, Wei M, Guo Y, Feng W, Yan L, Tao Y, Cai B, Zhang J, Li W, Li Y.   +11 more
europepmc   +1 more source

免疫学 [PDF]

, 1997
安居 輝人, 岸 裕幸, 村口 篤, 柴原 直利, 森 啓子   +4 more
core   +1 more source

Ferroptosis and tumor immunity. [PDF]

Zhong Nan Da Xue Xue Bao Yi Xue Ban
Tao Q, Liu N, Chen J, Wu J, Li J, Chen X, Peng C.   +6 more
europepmc   +1 more source

免疫学 [PDF]

, 2002
岸 裕幸, 廣田 早苗, 村口 篤, 松田 正, 金田 尚, 長田 拓哉   +5 more
core   +1 more source

Tear fluid participates in systemic immunity. [PDF]

Zhong Nan Da Xue Xue Bao Yi Xue Ban
Wu T, Wen X, Zhang J, Zhang Y, Fu M, Yi G.   +5 more
europepmc   +1 more source

免疫学 [PDF]

, 2001
岸 裕幸, 廣田 早苗, 村口 篤, 松田 正, 長田 拓哉   +4 more
core   +1 more source

[Clinical strategies for perioperative management of esophageal squamous cell carcinoma in the immunotherapy era]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Fu H, Shen L, Huang B, Ma S.
europepmc   +1 more source