Results 31 to 40 of about 8,159 (205)

Preparation and Application of Monoclonal Antibody against Puerarin [PDF]

open access: yes, 2012
葛根为豆科植物野葛或粉葛的干燥根,为常用中药之一,具有解肌退热、生津、透疹、升阳止泻的功效,多用于治疗外感发热、头痛、项强、消渴、麻疹不透、热痢、泄泻、高血压等症状。葛根素(Puerarin,PU)是葛根的主要活性成分,属于黄酮类化合物,药理作用广泛,是一种很有开发前景的药物。 本实验采用杂交瘤技术制备葛根素的单克隆抗体,在此基础上建立葛根素的酶联免疫吸附分析法检测技术,对葛根中药及中成药中葛根素的含量进行检测和评价。 主要的实验结果如下: (1)采用高碘酸钠法合成免疫人工抗原PU ...
吴俊杰
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Physicochemical Characteristics and Spoilage Bacteria of Modified Atmosphere Packaged Braised Chicken during Refrigeration [PDF]

open access: yes, 2023
In order to explore dynamic changes in the physicochemical characteristics and microbial diversity of modified atmosphere packaged braised chicken during refrigeration, high-throughput sequencing technology and traditional isolation and identification ...
ZHANG Li, MA Yunhao, WANG Ying, XU Baocai
core   +1 more source

Cytological evaluation, culture and genomics to evaluate the microbiome in healthy rabbit external ear canals

open access: yesVeterinary Dermatology, Volume 35, Issue 5, Page 479-491, October 2024.
Background – Lop‐eared rabbits may be predisposed to otitis externa (OE) as a consequence of their ear conformation. Although otoscopy, otic cytological evaluation, and culture are valuable tools in dogs and cats, published data on rabbits remain lacking. Hypothesis/Objectives – This study aimed to assess the utility of otoscopy and cytological results
Nikoleta Makri   +8 more
wiley   +1 more source

Studies on Kinmen kaoliag liquor brewing system and the application of its secondary production- yellow water [PDF]

open access: yes, 2015
在酿酒过程中,高粱是决定白酒质量优劣的重要因素。高粱中淀粉含量越多,出酒率越高。除了淀粉外,高粱果皮中单宁和籽粒中蛋白质、脂肪等,在发酵过程中会产生一些副产物,从而影响高粱酒的风味。本研究选了金选、两糯、兴湘、红梗和红糯等5个品种的高粱进行外观、生化性质等传统研究,结果表明两糯总淀粉及支链淀粉含量最高,金选单宁含量最高。为能分辨不同高粱品种,收集了5个不同品种的高粱作为实验样本,测定它们的近红外光谱,利用光谱特征对其进行定性定量分析,可快速鉴别出5种高粱之间的品种差异。利用高粱品种间SSR ...
陳志敏
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The dynamic space of aid relations in protracted internal displacement: the case of Sri Lanka's northern Muslims

open access: yesDisasters, Volume 48, Issue 3, July 2024.
Aid relations in protracted displacement comprise a diversity of actors with different influence and involvement over time. Building on the case of Sri Lanka's northern Muslim's expulsion from the north of the country in 1990, this paper investigates the dynamic space of aid relations in their drawn‐out internal displacement.
Cathrine Brun   +4 more
wiley   +1 more source

Selective Isolation, Identification and Biological Activity Investigation of Rare Actinomycetes from Rhizosphere Soil [PDF]

open access: yes, 2016
天然产物是新药制备的重要原料,绝大多数来源于微生物的天然产物都分离自放线菌,尤其是链霉菌。链霉菌是放线菌的重要组成成员,能合成结构类型丰富、生物活性多样的次级代谢产物,可用于创制抗生素、抗肿瘤药物、免疫抑制药物、抗虫药物等,在医疗、农业、食品等领域具有重要的应用价值。然而,随着对链霉菌持续的开发利用,目前从链霉菌中发现新型、高活性的次级代谢产物的几率在不断下降,来源于链霉菌的新药研发速度明显放缓。为了解决链霉菌活性次级代谢产物资源日趋枯竭的问题 ...
施金迪
core  

Optimization of mixed fermentation technology of Lactobacillus helveticus andLactobacillus rhamnosus based on high viable count [PDF]

open access: yes, 2016
以瑞士乳杆菌和鼠李糖乳杆菌混合发酵培养,利用两菌株细胞代谢的差异,以静置发酵24 h后得到的活菌数目为指标,通过单因素实验和正交实验,研究了瑞士乳杆菌和鼠李糖乳杆菌高活菌数混合发酵的最适发酵条件。结果表明,瑞士乳杆菌和鼠李糖乳杆菌高活菌数混合发酵的最优条件为:发酵温度37℃,初始pH=6.8,接种量6%,瑞士乳杆菌:鼠李糖乳杆菌=1∶2,瑞士乳杆菌优先接种3 h。最终得到乳酸菌总活菌数为7.2×10~9m L~(-1)。与在相同条件下单独发酵的瑞士乳杆菌和鼠李糖乳杆菌活菌数相比,分别提高了1∶8倍和10.
李敬龙   +3 more
core   +1 more source

赤大袋鼠馆气载需氧菌分离鉴定及致病性分析

open access: yes野生动物学报, 2019
本试验采用自然沉降法对南京红山森林动物园赤大袋鼠馆的运动场、缓冲道、饲养内舍、工作通道和饲料间5个区域一年四季的气载需氧菌浓度进行测定,通过16S rRNA基因和生理生化鉴定相结合的方法分析气载需氧菌的组成。通过动物致病性试验对所分离的细菌进行致病性分析,采用药敏试纸片法分析致病菌株的耐药情况。结果显示,赤大袋鼠馆各个区域一年四季气载需氧菌浓度与温度呈正相关。经生化鉴定及16S rRNA比对结果分析,共鉴定3大类细菌71株,其中葡萄球菌属共8种共37株,占分离菌的52.1%;肠杆菌科细菌9个属 ...
李俊娴 1 #李敏捷 2   +1 more
doaj  

中国豹源豚鼠气单胞菌的分子鉴定与毒力基因分析

open access: yes野生动物学报, 2015
从表现为急性出血性败血症的中国豹分离到一株细菌,为了鉴定这株细菌,并对其主要毒力基因进行检测分析,实验中进行了细菌的分离培养、生化鉴定及动物毒力试验; PCR扩增gyrB基因并结合系统发育树的构建和分析进行了菌种的分子鉴定; 对分离菌进行了气溶素(aerolysin,aer)、热敏感细胞肠毒素(heat-Labile cytotonic enterotoxin,alt)、细胞毒性肠毒素(cytotoxic enterotoxin,act)、热敏蛋白酶(temperature-sensitive ...
李婉萍 彭仕明 陈 武 胡 俊 李少基 单 芬
doaj  

小鱼仙草精油化学成分的GC-MS分析 [PDF]

open access: yes, 2002
福建省科委基金资助项目 (K20096);福建省自然科学基金项目;国家中医药管理局项目 (2000-J-P ...
林文群, 陈忠
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