Isolation and Identification of Pathogenic Fungi of Carambola Fruit and Study on Their Metabolites [PDF]
In order to understand the types, biological characteristics, and metabolites types of pathogenic fungi in carambola, the typical diseased parts of carambola fruit were collected for fungal isolation by tissue isolation methods. The fungi were identified
Jie LIN, Wei WEI, Xiang XU, Ziyi LIN
core +1 more source
Zhongguo shiyan zhenduanxue, 2015 目的探讨临床分离表皮葡萄球菌附属基因调节因子agr基因多态性,了解表皮葡萄球菌的分子特征。方法收集84株临床分离并经过全自动微生物鉴定系统准确鉴定的表皮葡萄球菌,通过PCR扩增esp和mecA基因准确鉴定和区分甲氧西林敏感表皮葡萄球菌（MSSE）和耐甲氧西林表皮葡萄球菌（MRSE）,采用多重PCR扩增表皮葡萄球菌的agr等位基因,分析agr基因多态性。结果 84株临床分离表皮葡萄球菌中,20株为MSSE,占23.80%,其中agrⅠ型5株,agrⅡ型2株,agrⅢ型2株,agr未分型11株 ...
杨永长 +5 more
doaj
Isolation, Identification and Antibacterial Screening of Spoilage Organism in Guilin Rice Noodles [PDF]
Guilin rice noodles belong to fresh wet rice noodles, which are difficult to be preserved due to their high moisture content and susceptibility to microbial contamination.
Chunxia HUANG +6 more
core +1 more source
[Isolation, identification and safety evaluation of Lactobacillus gasseri strain LGV03 isolated from the vagina of healthy women]. [PDF]
Nan Fang Yi Ke Da Xue Xue Bao, 2021 Su B +5 more
europepmc +1 more source
Isolation, identification and control of spoilage microorganisms in Xiangwei stewed bean [PDF]
Objective: To better control the growth of spoilage microorganisms, and ensure the food safety of Xiangwei Luodou dry. Methods: The putrefactive microorganisms in Xiangwei dried tofu were identified by plate scribing method, colony morphology observation,
LI Bingxin +5 more
core +1 more source
Zhongguo shiyan zhenduanxue, 2014 目的探索最佳的小鼠睾丸支持细胞分离、培养及鉴定方案。研究神经生长因子（nerve growth factor,NGF）对小鼠睾丸支持细胞生理状态的影响。方法通过胶原酶、胰酶等处理分离一周龄小白鼠睾丸支持细胞,通过油红O染色对分离得到的细胞进行鉴定;用加入NGF的培养基和对照培养基分别体外培养小鼠睾丸支持细胞,探索NGF对小鼠睾丸支持细胞生长情况的影响。结果分离得到了较高纯度的小鼠睾丸支持细胞,培养48小时后NGF组的小鼠睾丸支持细胞生长情况明显优于对照组。结论 NGF对小鼠睾丸支持细胞生长有着重要的作用。
付璐璐 +7 more
doaj
Study on the Isolation Identification and Antitumor Activity of a Phenol Derivative from Mangrove Fungus BYY-1 [PDF]
, 2011 [作者简介] 杜希萍( 1978—) ，女，讲师，从事天然产物化学方面的研究． 通讯作者: 苏文金( 1956—) ，男，教授，从事海洋药物与食品方向研究，E-mail: wjsu@ jmu. edu. cn．[中文文摘]采用硅胶柱层析、薄层层析、Sephadex LH-20柱层析从红树内生真菌BYY-1的次级代谢产物中分离到化合物B1.通过乙酰化、NMR、HR ESI-Q-TOF MS等技术鉴定了化合物B1的结构,确定其为首次从红树内生真菌中分离到的化合物 ...
徐庆妍 +4 more
core
Pure Culture and Ultra-high Deep Metagenomic Sequencing for Analysis of the Differences in Microbial Diversity of High Temperature Daqu from Two Distilleries in Maotai Town [PDF]
In this study, the microbial diversity of high temperature Daqu from different distilleries in Maotai town was analyzed by the pure culture technique and macrogenomic sequencing.
WANG Yurong, HOU Qiangchuan, TIAN Longxin, LIU Juzhen, ZHOU Jiaping, GUO Zhuang
core +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】建立体外成肌细胞分离培养、纯化和鉴定方法。【方法】40 只小鼠分 10 次采用分离消化法培养成肌细 胞,分别用 A、B、C、D 4组不同培养液培养,以 Ficoll梯度液纯化, 进行 Desmin 免疫组化鉴定。【结果】成肌细胞培养后经台盼 蓝检查成活率达 95%以上,在接种后 4～ 5 d增殖达高峰, A、B、C 3 组培养液对成肌细胞的培养效果无差异。Ficoll 分离纯化 的成肌细胞纯度较高,有 97%的成肌细胞 Desmin 胞浆呈阳性反应。【结果】采用 Ficoll纯化以国产血清培养,
王 训 +3 more
doaj
Studies on the Secondary Metabolites of Four Marine Microorganisms [PDF]
, 2012 寻找具有生物活性新天然产物是发现新药先导化合物的主要途径，许多临床常用的药物均来自于微生物次级代谢产物。微生物活性次级代谢产物主要由放线菌产生，其次就是真菌。海洋微生物能够产生种类繁多、结构新颖、活性多样的次级代谢产物，已成为发现新药先导化合物的重要来源。 本论文对1株海洋真菌和3株海洋放线菌的次级代谢产物进行了研究，共分离鉴定了28个化合物的结构，其中6个新化合物。 从海洋植物内生真菌Phomopsissp.A818固体YMG培养基发酵提取物中共分离鉴定了11个化合物的结构 ...
宋龙凤
core