Results 11 to 20 of about 1,241,592 (150)
用Taqman MGB探针研究中国人群TNF-α基因启动子区单核苷酸多态性 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2006 【目的】了解中国人群肿瘤坏死因子α（the tumour necrosis factor α，TNF-α）基因启动子区多态性。【方法】 随机选取20名深圳地区汉族健康体检者，采用两对引物PCR扩增TNF-α基因启动子区（-1389nt～＋125nt），对PCR产物进行序列分析，寻找单核苷酸多态性位点（single nueleofide polymorphisms，SNPs）。采用Taqman MGB探针建立了-857nt（C／T）位点的实时定量PCR（thereal-time PCR）分型方法 ...
庾蕾 +4 more
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Survivin基因启动子区-31C/G多态性与散发性 结直肠癌遗传易感性的关系
Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 探讨生存素Survivin基因启动子区-31C/G单核苷酸多态性与中国华南地区散发性结直肠癌(CRC)遗传易感性的关系65377; 【方法】 采用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)检测华南地区711例健康人和702例CRC的Survivin基因-31C/G位点单核苷酸多态性65377;【结果】 结直肠癌患者CC基因型的频率明显高于对照人群(36.35% vs.
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Journal of Diabetes, Volume 15, Issue 11, Page 987-993, November 2023., 2023 Highlights We used data from the genome‐wide association studies database and two‐sample mendelian randomization method, we found that genetic evidence supporting that knee osteoarthritis is a potential risk factor for type 2 diabetes. Abstract Background Physical inactivity is an independent risk factor for type 2 diabetes (T2D).
Xing Xing +3 more
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Evaluation and redesign of the primers for detecting nitrogen cycling genes in environments
Methods in Ecology and Evolution, Volume 13, Issue 9, Page 1976-1989, September 2022., 2022 Abstract A better understanding of how nitrogen (N) cycling genes are involved in ecological processes is one of the crucial areas of microbial ecology. Currently, most molecular biological techniques investigating N cycling genes in the environment heavily rely on the accuracy of the polymerase chain reaction (PCR) primers; however, their specificity ...
Zhujun Wang +12 more
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Journal of Diabetes, Volume 13, Issue 2, Page 143-153, February 2021., 2021 Highlights A machine learning model can significantly predict imminent development of islet autoimmunity based on environmental, genetic, and metabolic features. The machine learning algorithm feature selection identified type 1 diabetes‐associated single nucleotide polymorphisms from The Environmental Determinants of the Diabetes in the Young (TEDDY ...
Bobbie‐Jo M. Webb‐Robertson +10 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 2006 【目的】研究中国人群MEGSIN基因多态性及其分布特征，并与国外数据库进行比较。【方法】随机选取208例广东地区汉族个体，提取基因组DNA。对包含外显子、外显子-内含子交界区及5’UTR区、3’UTR区的PCR产物进行直接测序。综合正反向结果识别和鉴定基因内SNPs。所得结果在美国国立生物技术信息中心（NCBI）的SNP数据库（dbSNP）中进行查询和比较。【结果】在所有研究对象中共发现24个SNPs，主要位于非编码区；22个为替换型SNP，1个为插入型SNT，另一个为缺失型SNP ...
黄霜 +6 more
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Journal of Diabetes, Volume 11, Issue 11, Page 857-868, November 2019., 2019 Highlights Ten single nucleotide polymorphisms were found to be associated with diabetes risk. There were significant associations of diabetes genetic risk scores and weighted diabetes genetic risk scores with fasting plasma glucose and diabetes. Abstract Background Genome‐wide association studies have identified loci that significantly increase ...
Chao Song +11 more
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, 2018 为开发常见鲑鳟养殖物种通用的遗传分析工具,本研究利用Affymetrix虹鳟（Oncorhynchus mykiss） 57K高通量单核苷酸多态性（Single nucleotide polymorphism,SNP）芯片,对国内代表性鲑鳟养殖群体开展了分型检测,包括山女鳟（Oncorhynchus masou masou）、银鲑（Oncorhynchus kisutch）、美洲红点鲑（Salvelinus fontinalis）、白斑红点鲑（Salvelinus leucomaenis） 4个物种 ...
许建 +7 more
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Effects of Oxytetracycline on Population Growth and Genetic Diversity of Euplotesvannus (Marine Protist) [PDF]
, 2016 抗生素作为一类环境污染物在海岸带生境中广泛存在,它们对海洋原生生物遗传多样性的影响还不清楚.本工作以一种海洋纤毛虫原生生物(扇形游仆虫(Euplotes vannus))为例,研究了一种常见四环类抗生素(土霉素)对其生长的影响,并通过单细胞(个体)分析,探索了抗生素对其大核基因组中核糖体小亚基RNA基因(SSU r DNA)序列多样性的影响.结果表明,与对照组相比,3个土霉素处理组(1,10和20μg/m L)中纤毛虫的自然增长率随土霉素浓度升高显著降低,细胞大小则呈增大趋势.SSU r ...
龚骏 +3 more
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, 2020 组蛋白是染色体核小体的重要组分,在调控染色质结构、基因转录、个体发育等不同生物学过程中起着重要作用。为了研究鱼类组蛋白基因是否存在核苷酸的多态性以及组蛋白核苷酸多态性是否会影响鱼类的抗病力,本实验以斑马鱼和草鱼为研究对象,通过PCR扩增克隆了组蛋白H2A的全长开放阅读框;利用过表达技术、菌落平板计数、感染存活分析以及荧光定量PCR技术,研究了斑马鱼和草鱼组蛋白H2A核苷酸多态性不同变异体在杀鱼爱德华氏菌感染中的作用。在本研究中,实验发现鱼类组蛋白H2A存在着丰富的核苷酸多态性 ...
聂品, 昌鸣先, 方红, 武小曼
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