, 2007 选用TCA/丙酮沉淀法、缓冲液法、柱层析法、裂解液法和裂解液-超速离心法分别提取金乌贼视神经节蛋白质,其中蛋白质得率较高的方法有裂解液-超速离心法、裂解液法,其次为TCA/丙酮沉淀法.经双向凝胶电泳分离,并采用Melanie 4 Trial软件统计蛋白质斑点数目.发现pH 5~8范围的载体两性电解质适合于分离视神经节蛋白质组,其电泳图谱中多数蛋白质斑点清晰可见,显示出高分辩率、重复性好和易取样供质谱分析的优点,适合于开展视神经节蛋白质组学研究.裂解液 ...
黄慧英, 黄河清, 黄福生
core
Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 应用乙腈(ACN)预处理血清的方法,对肝细胞癌(HCC)患者的血清进行蛋白质组分析,寻找与HCC发病相关的蛋白质65377; 【方法】 收集原发性HCC患者和健康人的血清各12例,首先使用乙腈预处理,然后去除白蛋白和免疫球蛋白等高丰度蛋白质,再进行双向电泳(2-DE)分析,筛选HCC与健康对照血清中有显著性差异的蛋白质斑点,并进行MALDI-TOF/TOF质谱鉴定65377; 【结果】 血清经0%65380;20%和30%浓度的乙腈预处理后,进行2-DE分析发现蛋白质斑点的检出数量分别为532
doaj
[Binary encryption comprehensive experiment based on agarose gel electrophoresis]. [PDF]
Se PuDuan JW +7 more
europepmc +1 more source
[Progress in electric field assisted molecular imprinting technology]. [PDF]
Se PuWu JY, Huang XJ.
europepmc +1 more source
[Preparation of magnetic bifunctional materials for exosome capture from serum and phosphopeptide enrichment]. [PDF]
Se PuWang ZR, Zheng HJ, Jia Q.
europepmc +1 more source
[Protective effect and mechanism of TSPAN9-mediated mitocytosis in interleukin-1β-induced rat chondrocyte senescence]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiChen Q, Da W, Luo N, Shen B.
europepmc +1 more source
[Guidelines for the diagnosis and management of first relapsed multiple myeloma in China (2026)]. [PDF]
Zhonghua Xue Ye Xue Za ZhiPlasma Cell Disease Group +3 more
europepmc +1 more source
[Mefloquine HCl promotes DNA repair and alleviates radiation-induced lung epithelial cell injury]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoZhang Y, Wen Q, Huang H, Zhou M, Wang J.
europepmc +1 more source
[Pathogenic mechanisms of Leber hereditary optic neuropathy caused by m.3472T>C mutation]. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue BanZhang H, Shan W, Yasheng M, Guan M.
europepmc +1 more source
[Research progress on the pretreatment and analysis of prohibited components of glucocorticoids in cosmetics]. [PDF]
Se PuLiu L, Xiao XH, Fang JH, Li GK.
europepmc +1 more source