Study on Isolation, Purification and Thermal Stability of Cashew Allergen Ana o 3(腰果过敏原Ana o 3蛋白的分离纯化及热稳定性研究) [PDF]
Shipin kexue jishu xuebao, 2022 Ana o 3 is one of the main allergens of cashew nuts (Anacardium occidentale Linn.). Using raw cashew nuts as raw materials, the crude protein was extracted by crushing and degreasing, low-salt extraction.
XIAO Zhiwen(肖志文) +4 more
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Study on Interaction of Rutin and Ferulic Acid with Casein(芦丁和阿魏酸与酪蛋白的相互作用研究) [PDF]
Shipin kexue jishu xuebao, 2020 Interactions between phenolics and protein play important roles in the stability and bioactivities of functional milks enriched in phenolic compounds.
LEI Xuan(雷选) +3 more
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Inhibitory Mechanism of Ferulic Acid on Xanthine Oxidase(阿魏酸对黄嘌呤氧化酶的抑制机理) [PDF]
Shipin kexue jishu xuebao, 2023 In order to systematically explore the molecular mechanism of ferulic acid suppressing the activity of xanthine oxidase (XO), the effects of ferulic acid on the inhibitory reversibility, inhibition kinetics, binding properties, and structure were ...
ZHANG Nanhai(张南海) +7 more
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Effects of pH on Structure and Emulsibility of Catechin-Soy Protein Isolate Complex(pH值对儿茶素-大豆分离蛋白复合物结构与乳化性的影响) [PDF]
Shipin kexue jishu xuebao, 2023 Soy protein isolate (SPI) was used as raw material to prepare protein complexes loaded with catechins under different pH conditions. The interaction mechanism was explained by analyzing thermal stability, fluorescence quenching type, binding site number,
ZHAO Juyang(赵钜阳) +4 more
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De Novo Design and Antibacterial Activity of α-Helical Antimicrobial Peptide YHX-1(α-螺旋抗菌肽YHX-1的从头设计及抑菌活性研究) [PDF]
Shipin kexue jishu xuebao, 2022 Antimicrobial peptides can not only be used as biological preservatives to prevent microbial corruption in food processing and storage, but also be expected to alleviate the problem of drug resistance caused by antibiotic abuse because of its high ...
LI Jianxun(李剑勋) +6 more
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Isolation and Purification of Acid Phosphatase from Lateolabrax maculatus and Virtual Screening of Its Inhibitors(海鲈鱼酸性磷酸酶分离纯化及其抑制剂虚拟筛选) [PDF]
Shipin kexue jishu xuebao, 2023 To reduce the degradation of inosinic acid in marine fishes for maintaining the freshness of the fish, separation and purification of acid phosphatase (ACP) from Lateolabrax maculatus (sea bass) were performed using NaAc-HAc (pH 5.0) buffer extraction ...
LI Yuanyuan(李园园) +6 more
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Study on Antitumor Activity of Konjac Glucomannan from Rhizoma Amorphophalli(蛇六谷葡甘聚糖抗肿瘤研究) [PDF]
Shipin kexue jishu xuebao, 2014 The antitumor activity of konjac glucomannan (KGM) short chain polymer of rhizoma amorphophalli was discussed in this study. The results showed that KGM short chain polymer of rhizoma amorphophalli inhibited the growth of HeLa cells with the unobvious ...
HE Mingxiang(何明祥)
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Zhongguo youzhi, 2023 为改善植物蛋白的溶解性并提升其抗氧化活性，选择核桃蛋白、小麦蛋白和芝麻蛋白为实验对象，在碱性条件下与核桃衣多酚相互作用，考察了核桃衣多酚改性对3种植物蛋白中性条件下的溶解度和抗氧化活性的影响。此外，通过SDS-PAGE、圆二色光谱和荧光光谱对溶出蛋白质的结构变化进行表征。结果表明：在核桃衣多酚添加量为75 mg/g时，核桃蛋白、小麦蛋白和芝麻蛋白在中性条件下的溶解度分别提升了61.54、 61.23、69.25百分点；同时，添加核桃衣多酚改性后的核桃蛋白 ...
黄子林，陈思蕊，孔祥珍，张彩猛，华欲飞，陈业明，李兴飞HUANG Zilin，CHEN Sirui，KONG Xiangzhen，ZHANG Caimeng， HUA Yufei，CHEN Yeming，LI Xingfei
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Purification and Effect of Heat Treatment on Molecular Characteristics of Lactoferrin(牛乳乳铁蛋白的纯化及热处理对其分子特性的影响) [PDF]
Shipin kexue jishu xuebao, 2013 Lactoferrin is a kind of key basic proteins in bovine milk, which has attracted more and more research attentions. In the present study, lactoferrin was isolated from fresh bovine milk by defatting and casein-removing treatments, subsequently purified by
LIU Meng(刘 猛) +3 more
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Zhejiang Daxue xuebao. Lixue ban, 2007 利用内源荧光、1-苯氨基萘-8-磺酸(ANS)荧光、远紫外-圆二色光谱以及酶活力测定等手段研究了精氨酸激酶在1，1，1，3，3，3-六氟异丙醇(HFIP)溶液中变性后结构与活性的变化.为避免蛋白质聚沉的出现，实验中采用的变性剂浓度低于8%.当六氟异丙醇浓度为0.5%~1.5%时，蛋白质的二级结构和内源荧光发射峰位都没有发生明显的变化，而外源ANS荧光强度则显著增强.继续提高变性剂浓度至6%后，内源荧光发射峰位明显红移、二级结构含量增大、外源荧光减弱、酶活力完全丧失.内源荧光和外源ANS荧光的变化证明 ...
LIHai-long(李海龙) +3 more
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