Results 51 to 60 of about 38,507 (214)
瘦素受体基因Gln223Arg多态性与2型糖尿病合并慢性肾功衰竭关系
Zhongguo shiyan zhenduanxue, 2015 目的探讨瘦素受体基因Gln223Arg多态性与2型糖尿病合并慢性肾功衰竭关系。方法采用聚合酶链反应及限制性片段长度多态性方法(PCR-RFLP)分别测定126例2型糖尿病、108例2型糖尿病合并慢性肾功衰竭和185例对照组样本的瘦素受体基因Gln223Arg的基因型,同时检测各组不同基因型样本的血清瘦素水平。结果 2型糖尿病合并慢性肾功衰竭组与正常对照组瘦素受体基因Gln223Arg基因型频率及等位基因频率比较差异有显著性 ...
李莹 +7 more
doaj
The role of C-terminal region of Hepatitis B virus x protein on virus replication and cell death [PDF]
, 2016 在全球范围内，肝细胞癌（HCC）是临床上最常见的恶性肿瘤之一。乙型肝炎病毒（HBV）的持续感染是引起HCC的高危因素。乙型肝炎病毒（HBV）X蛋白（HBx）对于HBV的复制和HBV相关肝癌的发生发展起着重要的作用。本研究从HBV相关肝癌患者的癌、癌旁组织标本和血清标本出发，发现HBV相关肝癌组织的低复制性与HBx羧基端的截短突变有相关性。先前也有研究表明HBx羧基端截短突变体对于HBV的复制水平又不同程度的影响，但是所涉及的亚细胞结构靶点及参与的作用机制尚未阐明 ...
林碧芸
core
Conservation Biology, EarlyView.Abstract The Natura 2000 (N2K) network of protected areas is one of the main tools for area‐based conservation in the European Union (EU), yet its role in preserving plant biodiversity requires better understanding. We examined data kept in the European Vegetation Archive from over 1.2 million vegetation plots and obtained over 14.2 million plant ...
Michele Di Musciano +41 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2010 目的探讨雌激素受体-α（ERα）基因多态性与江西地区女孩性早熟的相关性。方法应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析技术,检测90例江西地区性早熟女孩和70例江西地区健康体检女孩ERα基因内含子1 Pvu Ⅱ T/C和XbaI A/G酶切位点基因多态性,并观察基因型对血清E2的影响。结果病例组ERα基因XbaⅠ基因型和等位基因频率分布与对照组相比差异有显著性 ...
柯江维 +6 more
doaj
Conservation Biology, EarlyView.Abstract Illegal wildlife trade (IWT) is one of the largest global illegal activities, and it negatively affects biodiversity and sustainable development worldwide. DNA barcoding coupled with high‐throughput sequencing (i.e., metabarcoding) is useful in identifying taxa affected by IWT and has been used routinely for decades.
Maria Joana Ferreira da Silva +9 more
wiley +1 more source
Cloning and expreesion of three smad genes and two homologous genes of TGF-β in Trichinella spiralis [PDF]
, 2015 旋毛虫是一种重要寄生虫,寄生于人、猪和鼠等多种动物体内产生致死性疾病。因旋毛虫病易感染，分布范围广泛和难治愈等特性，研究出能有效治疗旋毛虫病的制剂已成为全世界关注的热点。 哺乳动物中TGF-beta信号转导由TGF-β、Activin、inhibin、GDF、BMP和MIS等组成，TGF-beta参与细胞增殖分化、胚胎发育和骨形成的调控。在哺乳动物中TGF-beta信号途径的研究已经比较深入，但TGF-beta信号转导在旋毛虫中的报道还比较少。本文研究的smad-1和smad ...
刘燕
core
Conservation Biology, EarlyView.Abstract Consumer demand for ivory perpetuates the unsustainable and illegal killing of African elephants and other wildlife species. Interventions that aim to change consumer behavior are increasingly recognized as a crucial element of demand management. However, poor design and implementation have limited their effectiveness.
Molly R. C. Brown +2 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2010 目的研究非小细胞肺癌患者癌组织和血浆p16基因甲基化及其临床意义。方法选择120例非小细胞肺癌患者,用巢式甲基化特异性聚合酶链反应法检测其肺癌组织、癌旁正常组织和外周血血浆p16基因的甲基化,并对120例正常对照组血浆样品进行p16基因甲基化检测,各组检测结果进行比较。结果肺癌组织p16基因甲基化率44.2%,高于癌旁组织的11.7 ...
陈首慧 +8 more
doaj
Representation of obligate groundwater‐dwelling copepod diversity in European protected areas
Conservation Biology, EarlyView.Abstract Groundwaters sustain diverse surface ecosystems and are populated by metazoan species, mostly invertebrates, that provide fundamental ecological functions and are often of prominent conservation value due to narrow endemism and high phylogenetic rarity.
Francesco Cerasoli +7 more
wiley +1 more source
利用改良Nest-tetra-primerspecificPCR技术对两种石斑鱼的鉴别 [PDF]
, 2009 云纹石斑鱼(EPInEPHEluS MOArA)和褐石斑鱼(E.brunEuS)为石斑鱼属内近缘种,外形相似常被混淆.本研究改良建立了nEST-TETrA-PrIMEr SPECIfIC PCr方法,获得了云纹石斑鱼和褐石斑鱼线粒体dnAnd2基因内的3个特异性条带,分别为内参序列nC1(394bP)、特异性条带nd2-M(268bP)和nd2-b(122bP),以及核基因组中核糖体dnAITS1区的5个特异性条带,分别为内参序列nC2(588bP)、nC3(563bP),特异性条带rdnA-M ...
丁少雄 +4 more
core