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骨肉瘤细胞外泌体激活肺组织成纤维细胞炎性信号通路 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2020 目的本研究旨在研究骨肉瘤细胞来源外泌体对靶器官微环境的调控作用及其分子机制。方法提取骨肉瘤细胞143B外泌体并鉴定，通过PKH26荧光染色后经尾静脉注入裸鼠体内，24 h后提取肝、脾、肺、肾及脑组织，荧光显微镜下计数不同视野红色荧光数量；通过免疫荧光检测不同类型细胞中外泌体摄取情况；143B外泌体与人肺成纤维细胞共培养，荧光显微镜检测摄取情况，进一步利用qPCR检测炎性因子IL-1β、IL-6及TNF-α表达水平，通过蛋白印迹法检测炎性信号通路NF-κB通路p-p65和MAPK通路p-ERK及p ...
谢显彪 +7 more
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被α-synuclein激活的小胶质细胞通过外泌体转运miR-19a-3p抑制神经细胞自噬 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2021 目的研究被过表达α-synuclein的SH-SY5Y细胞外泌体刺激后的小胶质细胞重新分泌的外泌体（SNCA-HM Exo）的miRNAs成分变化，及其对神经细胞自噬的影响。方法收集小胶质细胞外泌体进行miRNAs芯片分析和PCR检测，筛选出差异表达的miRNAs，对其靶基因进行KEGG及GO分析，筛选出与PI3K/Akt/信号通路相关的miRNAs。将SH-SY5Y细胞分为四组，分别为Con-HM Exo组，Con-HM Exo+miR-19a mimic组，SNCA-HM Exo组以及SNCA-HM
林辉, 刘思齐, 黄云苑, 周天恩
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Research progress in surface plasmon resonance technology in exosome characterization and identification [PDF]
, 2023 Exosomes are lipid bilayer membrane vesicles that are widely distributed in peripheral blood， saliva， urine， ascites and other fluids. A variety of tumor-related genes in exosomes are involved in the information exchange between cancer cells and normal ...
Gong Ke, Su Yanqiong, Lu Kunjie, Xu Fengjuan, Xiao Wei, Cao Donglin
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Sequencing and Analysis of MicroRNAs in Bovine Milk Exosomes [PDF]
, 2023 In this study, density-gradient centrifugation was used to extract bovine milk exosomes, and the small non-coding RNA (sRNA) in the exosomes were sequenced by Illumina sequencing technology to explore the expression profile of bovine milk microRNAs ...
SHANG Jingwen, CHAI Yuxia, CAO Xueyan, YUE Xiqing, YANG Mei
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miR-185-5p facilitates intracellular Mycobacterium growth via inhibiting macrophage autophagy [PDF]
, 2023 Objective·To explore the regulatory effect of miR-185-5p on autophagy in Mycobacterium-infected macrophages and its influence on the survival of intracellular Mycobacterium.Methods·Macrophages derived from human monocytic-leukemia (THP-1) cells were ...
LIN Li +4 more
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Advances in the role of circular RNA in schizophrenia [PDF]
, 2023 Schizophrenia is a complex and severe mental disorder, the precise cause of which remains unknown. Circular RNA (circRNA), a type of non-coding RNA with a loop-like structure, is abundant in eukaryotic cell transcriptomes. Recent advancements
FU Lirong, ZHANG Chen
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外泌体介导miRNA-155靶向CTHRC1对胃癌细胞腹膜种植转移的影响
Zhongguo shiyan zhenduanxue, 2022 目的 探讨外泌体转运miRNA-155靶向胶原三螺旋重复蛋白1(CTHRC1)对胃癌细胞腹膜种植转移的影响。方法 提取胃癌BCG-823细胞外泌体并采用透射电子显微镜鉴定；实时荧光定量PCR检测胃癌BCG-823细胞和外泌体miRNA-155水平；激光共聚焦显微镜观察人腹膜间皮HMrSV5细胞对外泌体吞噬情况；设立阴性对照组、外泌体组和miRNA-155mimic组，通过划痕实验检测外泌体转运miRNA-155对腹膜间皮细胞HMrSV5迁移力的影响 ...
高正兴, 赵琳
doaj
Research progress of tumor-associated macrophages in immune microenvironment and targeted therapy of osteosarcoma [PDF]
, 2023 Osteosarcoma (OS) is a common primary malignant bone tumor in children and adolescents. The high recurrence and metastasis rate have become a common clinical problem to be solved, but there is no effective treatment.
CHEN Junjun +5 more
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Recent Developments in Stem Cells from Human Exfoliated Deciduous Teeth in the Treatment of Non-oral Diseases [PDF]
, 2023 Stem cells from human exfoliated deciduous teeth (SHEDs) are odontogenic stem cells with strong proliferation ability and multilineage differentiation potential.
JIANG Zhihan, XU Ling
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LncRNA HOTAIR和miR-122在肝细胞癌循环外泌体中的表达及与AFP、PIVKA-Ⅱ的关系
Zhongguo shiyan zhenduanxue, 2021 目的检测肝细胞癌(HCC)患者循环血样中外泌体LncRNA HOTAIR和miR-122表达情况与患者临床病理特征以及血清肿瘤标志物的关系。方法收集2019年1月-2020年1月在本院确诊的97例HCC患者,并选取50例非恶性病变患者和50例健康人的血清样本分别作为良性对照和正常对照组。提取受试者血清外泌体,采用QPCR法检测外泌体LncRNA HOTAIR和miR-122相对表达量,并检测血清甲胎蛋白(AFP)、异常凝血酶原Ⅱ(PIVKA-Ⅱ)水平。结果与健康对照组和良性对照组比较 ...
阮鹏, 李虹, 李勇, 田琪
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