Results 31 to 40 of about 583,411 (160)
Zhongguo shiyan zhenduanxue, 2021 目的构建骨肉瘤耐药细胞,探讨LIMK1(LIM Kinase1)逆转骨肉瘤耐药的作用及机制。方法通过长春新碱(VCR)诱导建立人成骨肉瘤MG63的耐药细胞亚系,应用半数抑制浓度公式计算IC50值及耐药指数评估细胞的耐药性;CCK-8法检测细胞增殖抑制率;相差倒置显微镜观察耐药细胞细胞形态的改变;PT-PCR实验检测耐药基因MDR1及其相关基因LIMK1的表达;转染pSUPER-LIMK1重组质粒,验证沉默LIMK1基因逆转MG63耐药的作用并分析其机制。结果本研究所构建的耐药细胞的耐药指数为7.01 ...
黄丽红 +4 more
doaj
Rare Metals, Volume 45, Issue 1, January 2026.ABSTRACT The escalating issue of drug‐resistant pathogens, largely driven by the overuse of conventional antibiotics, has become a significant global health threat. Developing alternative therapeutic strategies and fabricating natural biological enzyme materials that mimic the high efficiency, selectivity, and mild‐operating conditions of natural ...
Shuai He +11 more
wiley +1 more source
热化疗逆转人肝癌细胞系HepG2/ADM多药耐药性的实验研究
Zhongguo shiyan zhenduanxue, 2007 目的研究热化疗对多药耐药人肝癌细胞系HepG2/ADM耐药性的影响并探讨相关的机制。方法通过MTT法测定热化疗对多药耐药人肝癌细胞系HepG2/ADM耐药性影响;利用流式细胞仪检测细胞内ADM浓度变化。结果热化疗使多药耐药人肝癌细胞系HepG2/ADM耐药性降低;细胞内阿霉素浓度提高。结论热化疗具有逆转多药耐药人肝癌细胞系HepG2/ADM耐药性的作用,可能与细胞内阿霉素浓度提高有关。
李玉, 王宁, 孙涛, 宋丽艳
doaj
Zhongguo shiyan zhenduanxue, 2021 目的 建立人胃癌耐药细胞,为研究人胃癌耐药细胞的耐药机制以及为耐药肿瘤细胞的治疗提供耐药肿瘤细胞模型。方法 体外培养人胃癌细胞SGC-7901,用50μmol/L顺铂多次反复冲击,对冲击4次和6次的SGC-7901分别检测对顺铂的抑制率和多药耐药基因（MDR）及多药耐药特异基因片段的表达,并计算药物对细胞的半数抑制浓度（IC50）。结果 经4次和6次冲击后,受冲击的SGC-7901胃癌细胞对顺铂的抑制率明显高于亲本细胞,IC50为63.98μmol/L和66.34μmol/L,而亲本细胞SGC ...
刘玉侠 +6 more
doaj
To Ban or Not to Ban: The UK's Hamlet Moment With Farm Antibiotics
Politics &Policy, Volume 53, Issue 6, December 2025.ABSTRACT This article applies the Multiple Streams Framework (MSF) to examine post‐Brexit antimicrobial resistance (AMR) governance in UK agriculture, focusing on the contested regulation of prophylactic antibiotic use in farm animals. The study reveals how Brexit created a structural policy window, yet political and ideological dynamics rendered it ...
George Asiamah
wiley +1 more source
野生动物学报, 2022 为了分析火烈鸟(Phoenicopterus ruber)腹泻沙门菌(Salmonella)的致病性及耐药性情况,2016—2020年采集动物园中患腹泻火烈鸟的粪便、肛拭子和死亡火烈鸟的肝脏等病料组织102份,采用细菌分离鉴定和PCR方法进行沙门菌鉴定,采用人工感染小白鼠致病性试验和K-B药敏纸片法分别检测分离菌株的致病性和耐药表型情况。结果显示:从102份病料组织中分离得到89株沙门菌,其中75株沙门菌对小白鼠具有致病性,这75株沙门菌分离菌株对链霉素、阿莫西林、氨苄西林等14种药物耐药率在45.3 ...
邓丽玲 汪明权 李雨芮
doaj
Veterinary Dermatology, Volume 36, Issue 5, Page 621-629, October 2025.Background – Canine superficial bacterial folliculitis (SBF) is a common recurrent condition with a growing requirement for culture and susceptibility as a consequence of increased antimicrobial resistance and antimicrobial stewardship. Different opinions persist about the ideal technique for culture sample acquisition, which varies with lesion ...
Jason B. Pieper +3 more
wiley +1 more source
Veterinary Dermatology, Volume 36, Issue 5, Page 552-565, October 2025.Abstract Background Canine pyoderma is one of the most common presentations in small animal practice, frequently leading to antimicrobial prescribing. Objectives To provide clinicians with antimicrobial treatment guidelines for staphylococcal pyoderma, including those involving meticillin‐resistant staphylococci.
Anette Loeffler +9 more
wiley +1 more source
多药耐药对肝癌细胞增殖、凋亡、侵袭活性、耐药机制及丝裂原活化蛋白激酶表达的影响 [PDF]
, 2012 目的:通过化疗药物诱导建立多株人肝癌多药耐药细胞模型,探讨多药耐药获得对细胞增殖、凋亡、侵袭能力的影响,耐药机制及与丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路表达的相关性。方法:采用多柔比星(adriamycin,ADM)高浓度冲击和小剂量缓升2种方法诱导建立人肝癌细胞(HepG2、SMMC-7721和BEL-7402)耐药模型。比较耐药细胞株与亲本细胞株间的差异,主要包括采用生物发光法进行药物敏感实验并计算耐药指数;RT ...
丁鑫 +6 more
core
Veterinary Dermatology, Volume 36, Issue 3, Page 234-282, June 2025.Background – Canine pyoderma is one of the most common presentations in small animal practice, frequently leading to antimicrobial prescribing. Objectives – To provide clinicians with antimicrobial treatment guidelines for staphylococcal pyoderma, including those involving meticillin‐resistant staphylococci.
Anette Loeffler +9 more
wiley +1 more source