构建MS2-RIP方法用于鉴定lncRNA DANCR在肿瘤细胞中的相互作用分子
Zhongshan Daxue xuebao. Yixue kexue ban, 2021 目的为了探讨lncRNA DANCR在肿瘤细胞中的作用机制，本研究拟构建一种新的MS2-RIP方法用于鉴定DANCR的相互作用分子。方法利用MS2噬菌体衣壳蛋白可与噬菌体复制酶编码基因5’端一段由19个碱基组成的RNA茎环结构序列，即MS2结合位点（MS2 Binding Site，MS2 BS）高效结合的特点，构建串联表达MS2 BS和DANCR的过量表达质粒，通过MS2蛋白与MS2 BS的亲和作用，使DANCR得到富集，并且同步捕获与DANCR相互作用的分子，用于进一步分析鉴定 ...
巫孟师 +4 more
doaj
Exploration of the relationship between nicotinamide metabolism-related genes and osteoarthritis [PDF]
Objective·To explore the relationship between osteoarthritis and nicotinamide metabolism-related genes using bioinformatics analysis, and identify key genes with diagnostic value and therapeutic potential.Methods·By using "Osteoarthritis" as a search ...
DENG Qingsong +2 more
core +1 more source
Constructing adverse outcome pathway for per- and polyfluoroalkyl substances and retinoblastoma based on PI3K-AKT/MAPK signaling pathway [PDF]
BackgroundExposure to per- and polyfluoroalkyl substances (PFAS) is associated with various cancers, and recent studies suggest it may also increase the risk of retinoblastoma (RB) in newborns.
Minghao WANG +4 more
core +1 more source
基于转录组数据分析M2巨噬细胞在胶质瘤免疫微环境中的分子特征
Zhongguo shiyan zhenduanxue目的基于CGGA及TCGA数据库中的转录组数据，分析M2巨噬细胞在胶质瘤免疫微环境中的分子特征，为在胶质瘤中的免疫治疗提供新思路。方法通过“xCELL”R包计算每位患者免疫浸润细胞得分，并分析M2巨噬细胞与临床特征间的联系。利用Kaplan-Meier、ROC曲线及COX回归分析评估M2型巨噬细胞作为临床预后指标的潜力。此外，应用基因本体论（GO）、京都基因与基因组百科全书（KEGG）及基因集富集分析（GSEA）来揭示M2型巨噬细胞在肿瘤免疫微环境中的功能。最后 ...
黄卓彦 +3 more
doaj
SIRT7基因低表达胶质瘤细胞株代谢特征的NMR分析 [PDF]
, 2019 肿瘤是一种代谢疾病,癌基因表达对肿瘤细胞代谢的影响是目前肿瘤研究的热点之一.本文利用基于核磁共振氢谱(~1H NMR)的代谢组学方法对癌基因SIRT7低表达胶质瘤细胞株的代谢特征进行分析,寻找与SIRT7基因表达相关的特征性代谢物和代谢通路.分析结果表明,SIRT7基因低表达组与对照组细胞的代谢轮廓存在显著性差异,其细胞水溶性萃取物中有22种代谢物浓度发生明显变化.与对照组相比,SIRT7基因低表达胶质瘤细胞株中乳酸、甘氨酸、谷氨酸等12种代谢物浓度升高;缬氨酸、亮氨酸、赖氨酸等10种代谢物浓度降低 ...
杨文圣 +4 more
core
Shuitu Baochi Xuebao, 2004 对紫色土坡地6种种植模式下的径流泥沙与养分流失进行了观测统计分析,结果表明,6种种植模式下的径流量、泥沙流失量及养分流失量存在显著的差异,南方早熟梨 紫花苜蓿及柑橘 鸭茅两种果草模式与其它种植模式相比其径流过程曲线较为平滑,无突显的径流峰值点,径流量最少,两种果草模式各项养分流失量与传统顺坡种植模式相比减少了4～7倍不等;流失泥沙中的养份含量高于背景土壤和溶蚀携带,具有富集养分的特征,其富集比大多在1.3～2.4之间。
蒋光毅 史东梅 卢喜平 刘玉民
doaj
Exploring the Mechanism of Hippophae Fructus Anti-obesity through Network Pharmacology and Molecular Docking [PDF]
Objective: To investigate the active components, protein targets, and mechanisms underlying the anti-obesity effects of Hippophae fructus using network pharmacology and molecular docking techniques, and to validate its in vitro anti-obesity efficacy ...
Chun ZHANG +8 more
core +1 more source
Characterization of hub genes associated with ferroptosis in diabetic nephropathy [PDF]
Objective To identify hub genes associated with ferroptosis in the progression of diabetic nephropathy （DN） through bioinformatics analysis， offering novel insights into DN treatment.
ZHAO Sheng, LI Wenchuan, DONG Lan, LIAN Rong, LI Yuejiao, HE Feng
core +1 more source
Effects of exogenous IBA on antioxidant property and IAA biosynthesis pathway in cuttings of Ficus carica [PDF]
[Objective] Exploring the effects of suitable concentrations of indole butyric acid (IBA) on rooting and sprouting as well as on antioxidant property and gene expression related to growth hormone biosynthesis pathway of fig(Ficus carica L.) cuttings ...
Bahayiding Wufue +4 more
core +1 more source
Exploring the mechanism of platycodin D in inhibiting nasopharyngeal carcinoma based on network pharmacology combined with experimental validation [PDF]
Objective To delve into the underlying mechanism of platycodin D in inhibiting nasopharyngeal carcinoma (NPC) through a combination of network pharmacology and cellular experimental techniques.
CHEN Yanfang +7 more
core +1 more source