Results 21 to 30 of about 146,209 (140)
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 摘 要:【目的】 观察脂多糖(LPS)对巨噬细胞自噬的影响及p38/MAPK通路在其中的作用?【方法】 体外培养巨噬细胞株RAW264.7,分为对照组?饥饿状态激活自噬组?单纯LPS刺激组?LPS+P38抑制剂(SB203582)组和LPS+mTOR抑制剂(rapamycin)组?将前期构建的载体pcDNA3.1-GFP-LC3,转染RAW264.7,通过荧光显微镜观察各组细胞中自噬体形成情况?qRT-PCR方法检测各组中与细胞自噬相关的基因Atg5,Atg7,LC3-Ⅱ和Bnip3 ...
赖义明
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番茄红素抑制香烟烟雾提取物刺激巨噬细胞所致的炎症反应 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2018 【目的】探讨番茄红素对香烟烟雾提取物（CSE）所诱导的人单核细胞株（THP-1）巨噬细胞炎症因子生成的影响及其作用的分子机制。【方法】用100 μg/L 佛波酯（PMA）诱导THP-1细胞48 h，使其分化为巨噬细胞后，分为空白组、CSE 组和CSE+不同浓度番茄红素组（0.5、1和2 μmol/L）。采用ELISA法检测THP-1巨噬细胞培养基中白介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的含量，荧光探针DCFH-DA测定细胞内活性氧（ROS）生成水平，Western-blotting ...
邹军 +6 more
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巨噬细胞ABCA1对Ox-LDL诱导的炎症因子的调节及其意义 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】研究在氧化低密度脂蛋白(Ox-LDL)诱导作用下,ATP结合盒转运子A1(ABCA1)对巨噬细胞中细胞间黏附分子-1(ICAM-1)、单核细胞化学趋向蛋白-1(MCP-1)mRNA和蛋白质及白介素-1β(IL-1β)蛋白质表达的影响,在细胞水平证实ABCA1对动脉粥样硬化的影响。【方法】用氟波酯(PMA)刺激THP-1细胞使之转变为巨噬细胞,Ox-LDL(30μg/mL)刺激3、6、12、24h后,以荧光定量RT-PCR和Western蛋白印迹法及酶联免疫吸附法(ELISA)检测ABCA1 ...
郭志刚 +3 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】研究鼻咽癌中浸润的淋巴类细胞与癌细胞凋亡间的相关性。【方法】收集中山医科大学病理学教研室 38 例未经治疗的鼻咽癌活检标本。应用 TUNEL ( TdT-mediated dUTP Nick End Labeling)方法定量检测凋亡癌细胞计数(每 5 000 个 鼻咽癌细胞中的凋亡细胞数)。以免疫组化法分别检测T 细胞,单核/巨噬细胞,B 细胞和树突状细胞。【结果】① 在未经治疗 的鼻咽癌活检标本各例间癌细胞凋亡计数差异很大,平均为( 12. 9±13.
宗永生 +4 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 2019 【目的】探究锌离子在动脉粥样硬化巨噬细胞泡沫化及斑块形成过程中的影响及其机制。【方法】用氧化低密度脂蛋白（oxLDL）刺激THP-1细胞建立巨噬细胞泡沫化模型，用油红O染色法检测在0、30和60μmol/L锌浓度环境下泡沫化程度，用荧光标记法检测巨噬细胞对脂质的吞噬情况，用免疫印迹法检测相关清道夫蛋白CD36、SR-A蛋白的表达，用实时荧光定量PCR检测锌离子转运体的mRNA相对表达量。用ApoE-/-小鼠随机分4组：正常饲料组（Chowgroup）、高脂缺锌组（HFD-ZnD）、高脂正常锌组（HFD）、
田甜, 周芳敏, 汤勇波
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NLRP3炎症小体在甲型流感病毒H1N1预感染降低小鼠巨噬细胞抗MRSA免疫中的作用 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2020 目的研究NLRP3炎症小体在经甲型流感病毒H1N1预感染的小鼠巨噬细胞抗耐甲氧西林金黄色葡萄球菌（MRSA）免疫中的活性及作用。方法以MRSA分别感染小鼠巨噬细胞（MRSA组）和以甲流病毒H1N1预感染1周的巨噬细胞（H1N1+MRSA组），荧光定量PCR检测各组细胞NLRP3、Caspase-1、IL-1β的mRNA表达强度，免疫荧光及western blot检测细胞中NLRP3的蛋白表达强度，ELISA检测细胞上清液IL-1β的浓度。结果MRSA组NLRP3、Caspase ...
师小函 +5 more
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Journal of Diabetes, Volume 14, Issue 12, Page 806-814, December 2022., 2022 Highlights Metformin activates GDF15 expression, which reduces appetite with induction of weight loss in both diabetic and non‐diabetic patients. GDF15 level is linked and correlated with the progression of diabetic complications including thrombosis, diabetic nephropathy, diabetic neuropathy, and diabetic retinopathy. Abstract Type 2 diabetes mellitus
Hayder M. Al‐kuraishy +8 more
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Altered plasma cytokines in dogs with atopic dermatitis
Veterinary Dermatology, Volume 33, Issue 2, Page 131-e38, April 2022., 2022 Background – Canine (Canis lupus familiaris) atopic dermatitis (AD) shares similar clinical signs to human AD. The abnormal immune response of AD is orchestrated by T lymphocytes, and may include variable involvement of cytokines, Regulatory T (Treg) cells, eosinophils, mast cells and other immune components.
Hamutal Mazrier +3 more
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Journal of Diabetes, Volume 14, Issue 3, Page 192-204, March 2022., 2022 Highlights Many proinflammatory cytokines could be decreased after 1 week of intensive insulin therapy using continuous subcutaneous insulin infusion (CSII). This was the first report showing that the cytokines of interleukin‐6 receptor (IL‐6R), tissue inhibitor of metalloproteinase‐2 (TIMP‐2), platelet‐derived growth factor type BB (PDGF‐BB), and ...
Junyu He +6 more
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Cytotoxicity of Freshwater Clam Soup Incidental Nanoparticles and Effect on Phagocytosis of Murine Peritoneal Macrophages(河蚬汤纳米颗粒细胞毒性及对大鼠腹腔巨噬细胞吞噬功能的影响) [PDF]
Shipin kexue jishu xuebao, 2023 There are a large number of incidental nanoparticles endowed with special activities and functions by their nano-scale structures in some traditional food systems, but the effects of the incidental nanoparticles on the human cells remain unclear.
MA Qiuyun(马秋云) +6 more
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