Results 1 to 10 of about 1,791 (123)
Zhongshan Daxue xuebao. Yixue kexue ban, 2021 目的观察糖尿病合并脓毒症小鼠肠损伤特点，并初步探讨M1巨噬细胞在糖尿病合并脓毒症肠损伤中的作用。方法BKS-DB（Lepr ko/ko）和BKS-DB（Lepr wt/wt）雄性小鼠随机分为非糖尿病鼠假手术组（NDMS组）、非糖尿病鼠脓毒症模型组（NDMCLP组）、糖尿病鼠假手术组（DMS组）、糖尿病鼠脓毒症模型组（DMCLP组）4组，每组6只；细胞实验分为对照组（Sham组）、高糖组（HG组）、脂多糖组（LPS组）、高糖+脂多糖组（HG+LPS组）4组。采用盲肠结扎穿孔术（CLP ...
庞昌季 +5 more
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TLR4和IFN-γ激活的间充质干细胞通过调节巨噬细胞极化改善血吸虫病肝纤维化 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban目的探讨共激活的间充质干细胞（MSCs）是否通过调节巨噬细胞极化发挥治疗血吸虫病的作用。方法取20只成年Balb/c雄性小鼠，随机分为四组，分别为未感染组、感染组、MSC组和MSCTLR4+IFN-γ组。通过腹部敷贴尾蚴法建立日本血吸虫感染模型。在感染后第5周，经灌胃给予小鼠吡喹酮进行抗虫治疗；感染后第6周，分别给予不同处理的MSCs治疗或未治疗。每周监测各组小鼠体质量变化率。采用HE染色和Masson染色观察肝组织病理变化，通过RT-qPCR检测肝组织中α-SMA和胶原蛋白mRNA水平 ...
任瑶佳 +4 more
doaj +11 more sources
慢性乙型肝炎患者外周单核细胞衍生的巨噬细胞的极化状态与HBV DNA的相关性及其动态变化 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2022 目的为了研究慢性乙型肝炎（CHB）患者在核苷（酸）类（恩替卡韦（ETV）或替诺福韦（TDF））抗病毒治疗过程中外周单核细胞衍生的巨噬细胞 （MDMs） 的极化化状态的动态变化以及与HBV DNA、HBeAg的相关性。方法共纳入101例CHB患者和20名健康人的外周血标本。通过外周血单核细胞技术（PBMC）分离培养获得86例CHB患者和12例健康人的MDMs。采用流式细胞技术检测MDMs的特异性标志物（CD80，HLA-DR，CD163和CD206）的表达，从而评估MDMs的极化状态（即M1型 ...
陈晓彤 +7 more
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糖尿病足溃疡皮肤组织免疫失调和巨噬细胞重编程单细胞转录组分析 [PDF]
浙江大学学报. 医学版目的阐明糖尿病足溃疡（DFU）皮肤组织中巨噬细胞介导的炎症和组织损伤机制。方法收集DFU患者和非DFU者的皮肤组织标本，通过单细胞RNA测序获得79 272个高质量细胞转录组数据。采用无偏聚类方法识别细胞亚群，通过Seurat函数筛选DFU组与非DFU组间的差异表达基因，基因本体（GO）富集分析揭示基因功能，构建细胞间通信网络并进行配体-受体互作分析，从多维度揭示DFU微环境中细胞互作及信号调控机制。结果DFU皮肤组织中髓系细胞显著扩增，而内皮细胞、上皮细胞和平滑肌细胞等结构细胞显著减少 ...
黄 春丽 +5 more
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Effect of Tropomyosin of Macrobrachium nipponense on Mouse Macrophage Polarization Based on Transcriptomic Analysis(基于转录组学分析日本沼虾原肌球蛋白对小鼠巨噬细胞极化的影响) [PDF]
Shipin kexue jishu xuebao, 2022 To study the effect of tropomyosin of Macrobrachium nipponense on macrophage polarization, the differential analysis of gene expression during tropomyosin-induced macrophage polarization was analyzed by transcriptomic analysis.
HU Xin(胡昕) +4 more
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Ameliorative effects on osteoporosis of small extracellular vesicles derived from bone marrow mesenchymal stem cells [PDF]
, 2023 Objective·To investigate the effects of small extracellular vesicles (sEVs) derived from human bone marrow mesenchymal stem cells (BMSCs) on the regulation of osteoclast differentiation and macrophage polarization in mice, and mouse model of osteoporosis.
GUO Shangchun, LI Xuran, TAO Shicong
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Cytotoxicity of Freshwater Clam Soup Incidental Nanoparticles and Effect on Phagocytosis of Murine Peritoneal Macrophages(河蚬汤纳米颗粒细胞毒性及对大鼠腹腔巨噬细胞吞噬功能的影响) [PDF]
Shipin kexue jishu xuebao, 2023 There are a large number of incidental nanoparticles endowed with special activities and functions by their nano-scale structures in some traditional food systems, but the effects of the incidental nanoparticles on the human cells remain unclear.
MA Qiuyun(马秋云) +6 more
doaj +1 more source
SIRT2 regulates macrophage chemotaxis by de-modifying histone H4K8 lactylation [PDF]
, 2023 Objective·To explore the regulatory role of silent information regulator 2 (SIRT2) in modulating the immune phenotype of macrophages after infection by removing the lactylation at H4K8 site of histone and the corresponding mechanism.Methods·Human THP-1 ...
CAO Qing +4 more
core +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2023 目的研究丁香酚对人脐带间充质干细胞（HUC-MSCs）抑制肝星状细胞（HSCs）炎性活化以及巨噬细胞促炎表型作用的影响及其相关机制；方法体外培养并鉴定HUC-MSCs，并采用MTT法评估丁香酚对HUC-MSCs的毒力作用；体外划痕实验探究丁香酚对HUC-MSCs迁移能力的影响；丁香酚处理HUC-MSCs后的旁分泌产物（EU-MSCs-CM）、HUC-MSCs旁分泌产物（MSCs-CM）处理经TGF-β1处理活化的LX-2细胞，WB实验检测LX-2的α-SMA、COL1A1、Smad2/3、p-Smad2/
吴逐宇, 汪显耀, 陈艳, 何志旭
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Mechanism of alternative splicing in gene transcription and research progress in head and neck squamous cell carcinoma [PDF]
, 2023 Misregulation of alternative splicing may result in splicing defects in different types of cancers under multiple pathological conditions. Alternative splicing events may serve as potential molecular markers during the diagnosis and treatment for cancer.
Meng Guangxue, Ma Hong, Liao Dezhong
core +1 more source