Results 31 to 40 of about 1,031,548 (165)
This study provides an innovation to address the challenge in accessibility to samples and genetic markers in Snow leopard conservation genetic studies. We performed next‐generation sequencing (NGS) on 19 fecal samples obtained from the eastern Qinghai‐Tibet Plateau, and successfully assembled 17 complete mitogenome sequences (~16,720 bp) and ...
Chen Cheng +7 more
wiley +1 more source
四川山鹧鸪脑源性神经营养因子(BDNF)基因的扩增与序列分析
:参照人BDNF基因序列设计出一对引物 ,利用聚合酶链式反应 (PCR)方法 ,从四川山鹧鸪 (Arborophilarufipectus)基因组DNA中扩增到BDNF基因 .序列分析表明 ,该基因序列长 736bp ,与人和鸡BDNF基因的核苷酸同源性分别为 84 % ,95% ,推导的蛋白质序列与人和鸡相应序列的同源性分别为 92 %和 98% .实验扩增并分析了四川山鹧鸪的BDNF基因 ,为四川山鹧鸪的保护和繁育提供了重要资料 .
杨光垚 +5 more
doaj
External validation of QUiPP App in three independent European cohorts of symptomatic women
ABSTRACT Objective To validate externally the QUantitative Innovation in Predicting Preterm birth (QUiPP) App v.2 for the prediction of spontaneous preterm birth (sPTB) in symptomatic women attending tertiary care in Europe. Methods The QUiPP App v.2 was validated in three independent datasets: a prospective European multicenter cohort across five ...
A. M. Fischer +8 more
wiley +1 more source
基于 Linux 操作系统,利用 Phrap/Consed、crossmatch、blast 等软件和自主开发程序,构建了批量 ESTs 序列的检测系统,对麻疯树胚乳 cDNA 文库部分测序结果进行生物信息学分析,包括载体序列的去除、序列格式的转换、重复序列鉴定、序列的同源性比对、GO 生物学过程以及 GO 细胞组分分类.
熊瑛 +4 more
doaj
Updated insights into the molecular pathogenesis of canine atopic dermatitis
Abstract Atopic dermatitis (AD) is a common and chronic inflammatory skin disease with frequent relapses. The genomics revolution has greatly contributed and revolutionised our knowledge of human AD; understanding the molecular skin fingerprint of AD and associated pathogenic immune pathways has led to preclinical assessments of several novel ...
Frane Banovic
wiley +1 more source
Summary Repetitive DNA is a feature of all organisms, ranging from archaea and plants to humans. DNA repeats can be seen both in coding and in noncoding regions of the genome. Due to the recurring nature of the sequences, simple DNA repeats tend to be more prone to errors during replication and repair, resulting in variability in their unit length ...
Sridevi Sureshkumar +3 more
wiley +1 more source
针对波分复用-无源光网络(WDM-PON)的系统可容纳用户量问题,为扩大系统的容量和速率,同时保证光纤的带宽需求,文章提出了一种基于混沌扩频的光码分多址波分复用无源光网络(OCDMA-WDM-PON)系统方案,分析了Henon混沌序列作为地址码的初值敏感性、自相关和互相关等特性,并通过Matlab仿真平台分析了Henon混沌序列与Logistic混沌序列、光正交码(OOC)关于系统误码率(BER)性能的对比,验证了混沌序列在通信系统中作为地址码的可行性和优越性。实验结果表明 ...
杨立伟 +4 more
doaj
ABSTRACT This study examines the effects of the Covid‐19 pandemic on preexisting cash transfers and labor programs across Latin America, analyzing their temporal expansion and identifying the determinants of changes in their generosity and coverage, with a particular focus on the inclusion of informal workers.
Luis Vargas‐Faulbaum +2 more
wiley +1 more source
【目的】克隆并测定恶性疟原虫海南株(FCC1/ HN)肝期抗原1 基因(LS A-1 )3′端序列, 比较FCC1/ HN 株与国 外分离株LS A-1 3′端序列的差异。【方法】应用PCR 技术从FCC1/HN 株基因组DNA 中扩增LS A-1 3′端序列, 用T-A 克隆 法将其克隆入pMD18-T 载体。阳性克隆的重组质粒经酶切及PCR 扩增鉴定后, 用双脱氧链末端终止法进行基因序列测定。 应用DNAS TAR 软件进行不同分离株LS A-1 3′端序列的同源性分析。【结果】PCR ...
单志新 +5 more
doaj
ABSTRACT Objective To critically review the literature and synthesize evidence on the incremental yield of prenatal exome sequencing (PES) in fetuses with an apparently normal phenotype with a normal G‐banded karyotype or chromosomal microarray (CMA).
A. Sotiriadis +5 more
wiley +1 more source

