Results 31 to 40 of about 1,281,694 (159)

Linux平台下EST序列分析系统的构建应用实例

open access: yes四川大学学报. 自然科学版, 2005
利用抑制削减杂交和基因芯片技术获得一批黄孢原毛平革菌特异表达的EST序列,使用Phrap、EMBOSS、Blast、GENSCAN、MZEF软件,基于Linux操作系统,构建EST序列分析系统,完成了从EST和基因组Blast数据库的构建,载体序列的去除,EST序列的分类和组装,EST序列在基因组上的定位,外显子和内含子的识别以及基因预测.并通过使用perl语言结合bioperl模块写的脚本程序使分析过程自动化,从而可以快速地对大批EST序列进行分析 ...
李校, 江明锋, 张义正
doaj  

Metadiscourse and Teacher Moves for Supporting Student Reasoning in Linguistics Classroom Interactions

open access: yesInternational Journal of Applied Linguistics, Volume 36, Issue 1, Page 522-539, February 2026.
ABSTRACT This paper examines the features of metadiscourse across different teacher moves in university linguistics classroom discourse and the teaching strategies reflected in its use. The results reveal that teachers employ diverse metadiscourse during classroom interactions to support student reasoning.
Jingjie Li, Wenjie Hu
wiley   +1 more source

金融时间序列结构波动的子波变换分析

open access: yes四川大学学报. 自然科学版, 2007
运用子波变换的分析方法,研究金融时间序列多尺度局域结构的波动性.通过对具体的股指数据分析表明,利用子波分析法,不仅能准确判定出序列的稳定周期大小和相对强弱,而且能提取序列的局部波动和突发波动信息.
陈理, 袁晓, 汤韩杰, 帅晓飞
doaj  

DNA Barcoding Reveals Cryptic Diversity and Informs Conservation Priorities in Chinese Firmiana Species (Malvaceae) Using Genome Skimming Data DNA条形码揭示中国梧桐属 (锦葵科) 隐存多样性及保护优先级:来自基因组浅层测序数据的证据

open access: yesIntegrative Conservation, Volume 4, Issue 4, Page 569-583, December 2025.
Accurate species identification is critical for conserving endangered Firmiana trees in China. However, morphological similarities among closely related Firmiana species complicate its taxonomic identification. We conducted genome skimming on 62 samples representing all 10 Chinese species and discovered nrITS as the optimal DNA barcode, achieving 100 ...
Li‐Jun Yan   +8 more
wiley   +1 more source

麻疯树胚乳ESTs序列的克隆和生物信息学初步分析

open access: yes四川大学学报. 自然科学版, 2009
基于 Linux 操作系统,利用 Phrap/Consed、crossmatch、blast 等软件和自主开发程序,构建了批量 ESTs 序列的检测系统,对麻疯树胚乳 cDNA 文库部分测序结果进行生物信息学分析,包括载体序列的去除、序列格式的转换、重复序列鉴定、序列的同源性比对、GO 生物学过程以及 GO 细胞组分分类.
熊瑛   +4 more
doaj  

The Genome of Phoebe zhennan (Lauraceae) and Comparative Genomics of Phoebe 楠木(樟科)基因组与楠属植物比较基因组学研究

open access: yesIntegrative Conservation, Volume 4, Issue 4, Page 584-599, December 2025.
Phoebe zhennan is a Chinese endemic and valuable timber species of Phoebe (Lauraceae). We present a chromosome‐level genome assembly of P. zhennan. Our research provides novel insights into the genome evolution of Phoebe, including whole‐genome duplication, phylogenetic relationships of magnoliids, and key MYB regulatory genes involved in lignin ...
Mao Yameng   +3 more
wiley   +1 more source

四川山鹧鸪脑源性神经营养因子(BDNF)基因的扩增与序列分析

open access: yes四川大学学报. 自然科学版, 2000
:参照人BDNF基因序列设计出一对引物 ,利用聚合酶链式反应 (PCR)方法 ,从四川山鹧鸪 (Arborophilarufipectus)基因组DNA中扩增到BDNF基因 .序列分析表明 ,该基因序列长 736bp ,与人和鸡BDNF基因的核苷酸同源性分别为 84 % ,95% ,推导的蛋白质序列与人和鸡相应序列的同源性分别为 92 %和 98% .实验扩增并分析了四川山鹧鸪的BDNF基因 ,为四川山鹧鸪的保护和繁育提供了重要资料 .
杨光垚   +5 more
doaj  

A new beef: An analysis of discourse coalitions in the nascent cellular agriculture policy subsystem

open access: yesReview of Policy Research, Volume 42, Issue 6, Page 1404-1427, November 2025.
Abstract A robust approach to examining the nature and influence of coalitions in the policy process is the Advocacy Coalition Framework (ACF), which is especially useful to study policy issues fraught with scientific and technical controversy. Yet, important gaps exist in ACF literature regarding nascent policy subsystems. Consequently, there is scant
Nikki Nadeau, Ramiro Berardo
wiley   +1 more source

Reproductive outcome after frozen embryo transfer with hormone replacement therapy according to luteal‐phase support protocol: systematic review and network meta‐analysis of randomized controlled trials

open access: yesUltrasound in Obstetrics &Gynecology, Volume 66, Issue 4, Page 422-432, October 2025.
ABSTRACT Objective To compare reproductive outcome between luteal‐phase support (LPS) protocols for frozen embryo transfer (FET) cycles with hormone replacement therapy (HRT). Methods A search was conducted in MEDLINE, Scopus, LILACS, EMBASE, Scielo.br, PROSPERO, CINAHL, PsycINFO, AMED, ClinicalTrials.gov, ICTRP, the Cochrane Library and conference ...
A. Etrusco   +11 more
wiley   +1 more source

一种四倍体鲤科鱼Sox基因的克隆和序列分析 [PDF]

open access: yes, 2008
为了研究重要功能基因在多倍体基因组中的演化情况,通过分子克隆的方法从四倍体大鳞结鱼(Tordouronensis)中获得13条Sox基因序列,分别为SoxB,SoxC和SoxE组成员.对大鳞结鱼Sox基因系统进化分析的结果支持鱼类特异的基因组重复的假说,同时表明,基因Sox19可能为真骨鱼特有的Sox基因家族成员.序列分析显示,与其他物种相比大鳞结鱼中Sox基因的碱基替代,绝大多数为同义替代.结果表明,所得到的Sox基因序列均受到净化选择,同时由多倍化所造成的重复基因对Sox4a ...
李俊兵   +3 more
core  

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