Results 11 to 20 of about 1,031,548 (165)
基于基因组学的日本血吸虫序列分析平台的构建及应用 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 [目的]构建基于基因组学的日本血吸虫序列分析平台。[方法]基于工作站和Linux操作系统,利用公开的日本血吸虫或相关资源,收集有关生物信息学序列分析工具,并自行编写相关软件实现多种资源的有机整合。[结果]构建了本地化的日本血吸虫序列分析平台,在此基础上,自主设计了日本血吸虫EST序列的电子延伸系统、基于日本血吸虫EST数据库的同源全长cDNA序列检索系统和序列自动注释系统等。[结论]所构建的序列分析平台不仅可以完成常规的序列分析工作,还可成功地完成EST序列的电子延伸 ...
刘稳升 +5 more
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白纹伊蚊和致倦库蚊Wolbachia 16S rRNA 序列测定与分析 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2002 【目的】PCR 法扩增与测定白纹伊蚊和致倦库蚊的细胞内共生菌Wolbachia 16S rRNA 序列, 分析其种系发生关 系及传播方式。【方法】从单只雌蚊中提取DNA , PCR 法扩增16S rRNA 序列, 扩增产物进行克隆及测序。【结果】从白纹伊 蚊和致倦库蚊分别扩增出Wolbachia 16S rRNA 序列, 并克隆入pGEM-T 载体, 测序证明两序列长度分别为897 bp。【结论】 成功克隆了白纹伊蚊和致倦库蚊Wolbachia 16S rRNA 序列。测序和同源性分析表明 ...
葛春喜 +5 more
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基于经验模型分析2018年2月台湾花莲地震序列特征及其前震的成因机制
Dizhen xuebao, 2020 利用地震学的三个经典经验模型（古登堡-里克特定律、修正的大森定律和巴特定律）和描述前震活动的Dieterich前震模型对2018年2月中国台湾花莲地震序列的特征进行了分析。将该地震序列分为ML5.5前震序列、ML5.5余震序列和ML6.0余震序列等3段子序列进行研究，结果显示：利用古登堡-里克特定律得到的ML5.5余震序列和ML6.0余震序列的b值近似为1，ML5.5前震序列的b值近似为0.5；利用修正的大森定律得到的ML5.5余震序列和ML6.0余震序列的p值近似为0.9；利用修正的巴特定律得出ML5.
Yuancheng Shang, Baoping Shi
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Zhongguo shiyan zhenduanxue, 2022 目的 利用16SrRNA基因序列分析方法鉴定临床非典型细菌，为临床细菌分类鉴定新方法提供补充。方法提取细菌的DNA，自行设计引物并利用PCR技术扩增细菌16SrRNA基因片段，并对扩增片段进行序列测定，利用Blast等在线软件进行序列同源性分析。结果 在32株临床非典型细菌中，3株细菌可以鉴定到“属”，29株细菌可以鉴定到“种”，其中2株可以鉴定到“亚种”。结论 16SrRNA基因序列分析法是有效的病原细菌分类鉴定的补充。
白志鹏 +7 more
doaj
Wildlife Letters, EarlyView.Using a 3‐year, broad‐scale camera‐trap network in western Kansas, we tested whether coyotes and American badgers exhibit spatiotemporal patterns consistent with coordinated hunting. Despite high diel activity overlap, detection‐conditioned co‐detections occurred far less frequently than expected under independence, and short‐term temporal sequencing ...
Ty J. Werdel +3 more
wiley +1 more source
Methods in Ecology and Evolution, Volume 17, Issue 6, Page 1896-1909, June 2026.Abstract Molecular identification of insect food webs can accurately reveal complex trophic interactions and serve as a foundation for understanding ecosystem functioning and advancing biodiversity conservation. This process typically involves DNA library construction, high‐throughput sequencing and subsequent steps, including data assembly, annotation,
Xiao‐man Zhang +9 more
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Ultrasound in Obstetrics &Gynecology, Volume 67, Issue 4, Page 492-499, April 2026.ABSTRACT Objectives To assess the value of placental insufficiency markers for estimating the risk of genetic anomalies in fetuses with isolated early‐onset fetal growth restriction (FGR), defined as an estimated fetal weight ≤ 3rd percentile diagnosed ≤ 28 + 6 weeks' gestation.
M. Armengol‐Alsina +17 more
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Methods in Ecology and Evolution, Volume 17, Issue 4, Page 1143-1160, April 2026.Abstract DNA barcode‐based species identification has become pivotal in biodiversity research. Current barcoding‐based deep learning methods have advantages in species identification but exhibit significant limitations. By using COI barcoding alone, previous studies were unable to fully capture species identification features. Prior methods showed that
Bin Ye +4 more
wiley +1 more source
International Journal of Applied Linguistics, Volume 36, Issue 1, Page 522-539, February 2026.ABSTRACT This paper examines the features of metadiscourse across different teacher moves in university linguistics classroom discourse and the teaching strategies reflected in its use. The results reveal that teachers employ diverse metadiscourse during classroom interactions to support student reasoning.
Jingjie Li, Wenjie Hu
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Integrative Conservation, Volume 4, Issue 4, Page 569-583, December 2025.Accurate species identification is critical for conserving endangered Firmiana trees in China. However, morphological similarities among closely related Firmiana species complicate its taxonomic identification. We conducted genome skimming on 62 samples representing all 10 Chinese species and discovered nrITS as the optimal DNA barcode, achieving 100 ...
Li‐Jun Yan +8 more
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