甲状腺功能亢进激活心肌细胞β-catenin/FoxO1诱导大鼠心室重构的作用 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban目的探究甲亢激活心肌细胞β-catenin/FoxO1诱导大鼠心室重构的作用和机制。方法通过甲状腺激素（T4 0.1 mg/kg/day；腹腔注射）连续给药30 d构建甲亢诱导心室重构大鼠体内模型。利用β-catenin抑制剂MSAB（14 mg/kg）同时给药30 d，通过Western-blot检测心肌肥厚主要标志物ANP以及β-catenin、FoxO1等蛋白的表达情况；免疫荧光检测β-catenin、FoxO1的核内外表达及分布情况。利用原代培养的乳鼠心肌细胞，使用甲状腺激素（T3 20 ...
袁勋 +7 more
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MicroRNA-99b-5p通过抑制成纤维细胞生长因子21表达促进心肌细胞肥大
Zhongshan Daxue xuebao. Yixue kexue ban, 2022 目的研究微小RNA microRNA-99b-5p（miR-99b-5p）对心肌细胞肥大表型的调节作用及机制。方法实时荧光定量PCR（RT-qPCR）检测人心肌组织（包括健康对照者、心力衰竭患者心肌组织）以及横向主动脉缩窄（TAC） 手术小鼠心肌中miR-99b-5p的表达水平。血管紧张素Ⅱ（AngⅡ）处理原代乳小鼠心肌细胞（NMVCs）后，利用鬼笔环肽染色呈现心肌细胞大小，RT-qPCR检测miR-99b-5p表达水平。利用RT-qPCR及蛋白质免疫印迹法检测转染miR-99b-5p ...
陈丽文 +7 more
doaj
Pathological Changes and Cause of Death Associated with the Global Novel Coronavirus Disease （COVID-19） [PDF]
, 2023 The coronavirus disease 2019 （COVID-19） has been a global epidemic for more than three years, causing more than 6.9 million deaths. COVID-19 has the clinical characteristics of strong infectivity and long incubation period, and can cause multi-system ...
Bin YANG, Lu-yao XU, Ling-yue LI, Dong-fang QIAO, Si-hao DU, Xia YUE, Hui-jun WANG
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The effect of angiotensin (1-7) on bone marrow stem cells:adjunctive pharmacological therapy for cell transplantation in heart failure [PDF]
, 2008 Myocardial infarction [MI] is the major cause of death in western countries. The loss of cardiomyocytes and insufficient blood supply are the most frequent causes for the development of chronic heart failure [CHF]. Current therapy in patients after MI is
Qian, Cheng
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Expression of Cx43 and Cx45 in Cardiomyocytes of an Overworked Rat Model [PDF]
, 2019 Objective To study the effect of overwork stress response on the expression of connexin 43（Cx43） and connexin 45（Cx45） in cardiomyocytes and on cardiac function.
YANG Bo-fan, SHI Jing-zhuo, LI Jing, et al.
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Effects of hexabromocyclododecane (HBCD) on cardiac development in zebrafish embryos (Danio rerio): the mechanisms involved [PDF]
, 2015 六溴环十二烷（1,2,5,6,9,10-Hexabromocyclododecane，HBCD）是被运用最广泛的阻燃剂之一，在全球的用量仅次于四溴双酚A和多溴联苯醚。HBCD具有持久性、生物累积性和生物放大效应，因而被定义为持久性有机污染物（persistentorganicpollutants，POPs）。已有研究表明HBCD具有发育毒性、神经毒性、内分泌毒性和生殖毒性，但对心脏发育毒性的研究十分有限，尤其缺乏对心脏发育毒性机制的研究。本研究以斑马鱼和H9C2细胞系为实验模型，采用低浓度HBCD（0 ...
吴美芳
core
Regulatory effect of 3'-tRF-PheGAA on Ang Ⅱ-induced cardiomyocyte hypertrophy and its mechanism [PDF]
Objective To investigate the regulatory effect of 3'-tRF-PheGAA on angiotensin Ⅱ (Ang Ⅱ)-induced cardiomyocyte hypertrophy and its mechanism. Methods Primary cardiomyocytes of neonatal mice were obtained and cultured for 24 h, and were then divided into ...
CHENG Xueli, WANG Kai, ZHAO Yan, WANG Kun
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The effect of thyroxine on ATP-sensitive potassium channels and cardiolipin synthesis [PDF]
, 2015 甲亢性心脏病（HyperthyroidHeartDisease,HHD）是甲状腺功能亢进（Hyperthyrodism）的严重并发症，严重危害患者的生命健康，但其机制尚未完全阐明。心磷脂（Cardiolipin，CL）是主要位于线粒体内膜的特殊磷脂，在维持正常的线粒体功能中具有至关重要的作用。本课题组前期研究表明甲亢小鼠心脏中心磷脂含量升高，并且心磷脂参与甲亢性心脏病的病理过程。此外，文献资料显示甲亢会引起心磷脂侧链的C18:2的丢失，同时引起侧链中多不饱和脂肪酸含量的增多 ...
侯琦
core
A comparison Study of AgII induced cardiac hypertrophy model in TR3+/+ and TR3-/- mice by echocardiography [PDF]
, 2012 目的应用高频超声对比研究血管紧张素II（angiotensinⅡ，AngII）诱导的TR3敲除C57BL/6小鼠（TR3-/-小鼠）和野生型C57BL/6小鼠（TR3+/+小鼠）心肌肥大后其心肌厚度变化，结合HE染色、Westernblotting等试验方法，初步探讨TR3是否参与了心肌肥大的过程。方法将26只TR3-/-小鼠及33只TR3+/+小鼠根据是否给予血管紧张素II（AngII）按随机原则各分为2组，即：TR3+/+＋AngII组（27只）、TR3+/+＋PBS组（6只）、TR3 ...
张楠
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Regulation of Pol II pausing release and 3’-end mRNA cleavage by different subtypes of SEC [PDF]
, 2015 编码蛋白的真核细胞基因的转录是由RNAPolymeraseII通过一系列的高度复杂有序的调控机制完成的，整个转录的循环主要分为起始、延伸以及终止三个基本环节，其中延伸是mRNA转录中至关重要的一步，它决定了mRNA在基因转录中的合成速率。mRNA的转录延伸是由正性和负性转录延伸因子共同参与调控的，当mRNA转录出了大概20-30nt的时候，负性转录延伸因子NELF和DRB敏感因子DSIF通过跟RNApolymeraseII(PolII)的结合，使得PolII先暂停下来，正性转录延伸因子P ...
朱鑫星
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