慢病毒载体介导的BDNF基因与电针对神经病理痛模型大鼠痛阈的影响
Zhongguo shiyan zhenduanxue, 2011 目的观察慢病毒载体介导的BDNF与电针对神经病理痛模型大鼠热辐射痛阈的影响差别,比较基因与电针的镇痛疗效,探讨BDNF对疼痛可能的作用机理。方法本研究采用坐骨神经结扎损伤的神经病理性疼痛大鼠CCI模型,运用荧光免疫标记法,观察慢病毒载体介导的BDNF与电针干预后大鼠不同时间点（3 d、7 d、21 d）BDNF在相应节段脊髓内表达的变化。另运用BME-410C热痛刺激仪记录大鼠术前（0 d）及术后1 d、3 d、5 d、7 d、9 d、14 d、21 d,各组的热辐射刺激缩足潜伏期痛阈的变化 ...
黄爱苹 +5 more
doaj
Tc17细胞对乙肝相关慢加急性肝衰竭病情进展及乙肝病毒清除的影响
Zhongshan Daxue xuebao. Yixue kexue ban, 2019 【目的】探讨Tc17细胞表达对乙肝相关慢加急性肝衰竭（HBV-ACLF）病情进展及乙肝病毒清除的影响。【方法】纳入43例HBV-ACLF患者，以20例慢性乙型肝炎患者（CHB）和12例健康体检者（NC）为对照，流式细胞术检测外周血中Tc17细胞的表达，荧光定量PCR法检测HBV-DNA载量，采用MELD、MELD-Na、CLIF-CACLF及AARC评分等指标评估患者病情，分析Tc17细胞表达对病情进展及乙肝病毒清除的影响。【结果】与NC组及CHB组比较，HBV-ACLF患者Tc17细胞表达明显升高 ...
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 探讨抑癌基因PTEN对人慢性粒细胞白血病细胞系K562细胞周期的影响65377; 【方法】 将携带有野生型PTEN和绿色荧光蛋白的腺病毒(Ad-PTEN-GFP)及对照载体腺病毒(Ad-GFP)转染人慢性粒细胞白血病细胞系K56265377;MTT检测细胞增殖抑制率;流式细胞仪检测转染效率65380;细胞凋亡率和细胞周期的变化;荧光定量PCR(FQ-PCR)检测PTEN65380;Cyclin D165380;Cyclin D265380;CDK465380;P27Kip1 mRNA水平变化 ...
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 研究荧光素酶作为报告基因在活体定量和定位监测转基因人胚胎干细胞移植的动向的可行性65377; 【方法】 酶切pGL3 base质粒获得荧光素酶基因,酶切LTBR-uGIP获得载体骨架,连接荧光素酶基因和载体骨架构建慢病毒载体pULP,按照常规方法包装和扩增65377;ULP转导293T细胞以检测其荧光素酶的表达65377;人胚胎干细胞H9 (P40)以W3R细胞为饲养层常规培养,人胚胎干细胞转导iDuet101-IDO (吲哚胺2,3双氧化酶,IDO) 或iDuet101-CTLA4Ig ...
doaj
[Construction of a highly efficient HBV infection cell model based on HBV co-receptor neuropilin-1]. [PDF]
Zhonghua Gan Zang Bing Za ZhiLiu ZH, He XM, Xu KX, Yu HB, Chen J.
europepmc +1 more source
[Research Progress on Quorum Sensing-Regulated Oral Microbial Interaction Networks in Periodontitis]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanLi L, Yu Z, Xu Y.
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[Advances in clinical features, diagnosis, and treatment of pre-acute-on-chronic liver failure]. [PDF]
Zhonghua Gan Zang Bing Za ZhiNie YH, Cai DC.
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[Helicid alleviates depression-like behavior in rats with chronic unpredictable mild stress through the NCALD/sGC/cGMP/PKG axis]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoDU X +8 more
europepmc +1 more source
[Research on tissue regeneration and engineered scaffold materials in prevention and treatment of lymphedema]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiYuan S, Deng C.
europepmc +1 more source
[EVA1A overexpression improves non-alcoholic fatty liver disease in mice by regulating lipid metabolism and promoting lipophagy]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoXu J +8 more
europepmc +1 more source