Results 91 to 100 of about 1,677 (149)
Zhongguo shiyan zhenduanxue, 2012 目的已有研究显示HER2基因过表达乳腺癌细胞对曲妥珠单抗（TZB）产生抵抗性与LC3的过表达相关,本研究通过RNAi沉默耐TZB的SKBR3（SKBR3-TR）细胞LC3的表达,探讨HER2阳性乳腺癌对TZB抵抗的机制。方法以SKBR3乳腺癌细胞及SKBR3-TR细胞为研究对象,检测两组细胞中LC3表达情况。以SKBR3-TR细胞为研究对象,通过慢病毒载体沉默SKBR3-TR细胞LC3的表达,实时定量PCR（Realtime20PCR）、蛋白免疫印迹检测LC3mRNA、蛋白的表达及水平,通过CCK ...
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Tc17细胞对乙肝相关慢加急性肝衰竭病情进展及乙肝病毒清除的影响
Zhongshan Daxue xuebao. Yixue kexue ban, 2019 【目的】探讨Tc17细胞表达对乙肝相关慢加急性肝衰竭（HBV-ACLF）病情进展及乙肝病毒清除的影响。【方法】纳入43例HBV-ACLF患者，以20例慢性乙型肝炎患者（CHB）和12例健康体检者（NC）为对照，流式细胞术检测外周血中Tc17细胞的表达，荧光定量PCR法检测HBV-DNA载量，采用MELD、MELD-Na、CLIF-CACLF及AARC评分等指标评估患者病情，分析Tc17细胞表达对病情进展及乙肝病毒清除的影响。【结果】与NC组及CHB组比较，HBV-ACLF患者Tc17细胞表达明显升高 ...
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Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 探讨抑癌基因PTEN对人慢性粒细胞白血病细胞系K562细胞周期的影响65377; 【方法】 将携带有野生型PTEN和绿色荧光蛋白的腺病毒(Ad-PTEN-GFP)及对照载体腺病毒(Ad-GFP)转染人慢性粒细胞白血病细胞系K56265377;MTT检测细胞增殖抑制率;流式细胞仪检测转染效率65380;细胞凋亡率和细胞周期的变化;荧光定量PCR(FQ-PCR)检测PTEN65380;Cyclin D165380;Cyclin D265380;CDK465380;P27Kip1 mRNA水平变化 ...
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[Helicid alleviates depression-like behavior in rats with chronic unpredictable mild stress through the NCALD/sGC/cGMP/PKG axis]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoDU X +8 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 研究荧光素酶作为报告基因在活体定量和定位监测转基因人胚胎干细胞移植的动向的可行性65377; 【方法】 酶切pGL3 base质粒获得荧光素酶基因,酶切LTBR-uGIP获得载体骨架,连接荧光素酶基因和载体骨架构建慢病毒载体pULP,按照常规方法包装和扩增65377;ULP转导293T细胞以检测其荧光素酶的表达65377;人胚胎干细胞H9 (P40)以W3R细胞为饲养层常规培养,人胚胎干细胞转导iDuet101-IDO (吲哚胺2,3双氧化酶,IDO) 或iDuet101-CTLA4Ig ...
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[Application and advances of exosome-hydrogel system in wound healing]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiQiu Y, Tan Q.
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[EVA1A overexpression improves non-alcoholic fatty liver disease in mice by regulating lipid metabolism and promoting lipophagy]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoXu J +8 more
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[Application and research progress of metal-organic framework materials in skin repair]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za ZhiMa S, Wu W, Liu H, Liu M, Xing F.
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[Knockdown of <i>Cav1</i> inhibits mitochondrial function and mRNA m<sup>6</sup>A modification and expression of key genes in mouse hepatocytes]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoDing S +4 more
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[Advancement in neutrophil-based drug delivery systems]. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue BanZhou Z, Tang L.
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