Effect of Piezo1 on osteogenic differentiation of mouse bone marrow mesenchymal stem cells C3H10T1/2 based on CRISPR/Cas9 [PDF]
, 2023 Objective·To investigate the effect of Piezo1 on osteogenic differentiation of mouse mesenchymal stem cells C3H10T1/2 cell line based on CRISPR/Cas9 system that can achieve stable gene knockout.Methods·According to the principle of CRISPR/Cas9 target ...
DAI Qinggang +4 more
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Research progress in the relationship between mitochondrial dysfunction and osteoporosis [PDF]
, 2023 Osteoporosis (OP) is a chronic senile bone disease characterized by decreased bone mass and increased bone fragility. There are many inducing factors and the pathogenesis is complex. To explore the mechanism of OP and improve clinical efficacy has always
CHEN Yantong +4 more
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Advances in Chinese medicine-mediated AMPK/mTOR signaling pathway for the treatment of osteoporosis [PDF]
, 2023 Osteoporosis （OP） is a systemic bone disease and a common orthopedic disorder in elderly patients. High morbidity， disability and mortality rates and medical cost of OP bring huge burden to the patients and their family.
Zhang Wenhao, Yi Lin, Xiang Wenyuan, Remira·Aimaiti, Deng Yingjie, Liao Jun, Liang Zhiquan, Li Leijiang, Fang Rui
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Research progress in the roles of Notch signaling pathway during fracture healing [PDF]
, 2023 Fracture is the most common large-organ injury in humans. It takes several months or even longer from the onset to the completion of bone reconstruction. In severe cases, delayed healing or even bone discontinuity can occur.
GUO Liqiang, SHU Bing, ZHAO Shitian
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, 2019 石墨烯(Graphene)是一种平面的碳原子单层,呈二维六角形结构,具有非凡的电、热和物理性能。此外,石墨烯的分子结构可以用相关分子进行化学修饰,使电子器件拥有较高的性能。尽管碳衍生物已广泛应用于工业和电子领域,但其在组织工程(Tissue engineering)与再生医学(Regenerative medicine)中的应用尚处于研究阶段。研究表明,石墨烯具有良好的生物相容性、低毒性和较大的负载能力。本文综述了石墨烯及其相关材料诱导干细胞(stem cells)分化为成骨细胞(Osteoblasts)
李健, 李林峰
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长链非编码RNA UCA1促进人骨髓间充质干细胞成骨分化研究
Zhongguo shiyan zhenduanxue, 2023 目的 探讨lncRNA UCA1对人骨髓间充质干细胞成骨分化的影响。方法 构建过表达lncRNA UCA1的慢病毒载体(UCA1a和UCA1b)和空载体分别感染人骨髓间充质干细胞。实验分为4组：对照组1为干细胞对照组；对照组2为空载体对照组；UCA1a为高表达UCA1a的实验组；UCA1b为高表达UCA1b的实验组。在成骨诱导分化7 d、14 d、21 d时，进行茜素红染色。采用Real-Time RT-PCR检测成骨相关基因Col1、ALP、OPN和SP7的mRNA表达水平。结果 ...
卢倩倩, 张泽兵, 王景云
doaj
黄芩素对MC3T3-E1体外成骨分化及对口腔常见菌的抑菌作用 [PDF]
目的研究不同浓度黄芩素对MCT3-E1增殖及生物学行为的影响，以及其对口腔常见菌的抑菌作用，并探讨其相关机制。方法将MC3T3-E1细胞分别培养在0、6、12、18、24 μmol/L的黄芩素浓度下，通过CCK-8实验检测黄芩素处理后MC3T3-E1的增殖活性；对成骨诱导培养后的MC3T3-E1进行ALP活性检测；RT-PCR法检测RunX2、BMP2、Osterix基因的表达差异。K-B纸片法检测黄芩素处理24 h对大肠杆菌，金黄色葡萄球菌，血链球菌的抑菌效果。结果黄芩素可在一定程度降低细胞增殖活力 ...
周志迎 +4 more
core +4 more sources
Zhejiang Daxue xuebao. Lixue ban, 2008 对纳米羟基磷灰石颗粒对成骨前体细胞MC3T3E1的增殖和分化的影响进行了研究.采用四唑盐比色法(MTT)、碱性磷酸酶检测及矿化结节染色的方法分析了羟基磷灰石纳米颗粒悬液对成骨前体细胞的增殖和分化的影响.同时用纳米羟基磷灰石悬液的浸出液及吸附条件培养液后的纳米颗粒制备悬液分别处理细胞，探讨钙离子释放和蛋白吸附在成骨前体细胞增殖和分化过程中的作用.结果表明：在0~50 µg • mL-1，纳米羟基磷灰石颗粒对成骨前体细胞的增殖有一定抑制作用，且随着浓度的增加抑制作用也相应加强.10 µg • mL ...
WUYuan(吴苑) +5 more
doaj +1 more source
Dual-directional effect of all-trans retinoic acid on osteogenic differentiation of jaw bone marrow mesenchymal stem cells in vitro [PDF]
Objective·To explore the effect of all-trans retinoic acid (ATRA) of different concentrations on osteogenic differentiation of jaw bone mesenchymal stem cells (jBMSCs) in rats.Methods·jBMSCs from 4-week-old Sprague-Dawley (SD) rats were isolated and ...
DAI Qinggang +4 more
core +1 more source
Promotion of Nd:YAP laser biostimulation on the proliferation and osteogenic differentiation of human periodontal ligament cells through WNT/β-catenin signaling pathway [PDF]
Objective·To study the effect of neodymium-doped yttrium aluminum perovskite (Nd:YAP) laser biostimulation on the proliferation and osteogenic differentiation of human periodontal ligament cells (hPDLCs) and its possible mechanism.Methods·Five premolars ...
JIANG Lishan +3 more
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