重组腺病毒载体Ad-LMP-1的构建及其转染 MSC后成骨活性
Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 快速构建大鼠LIM矿化蛋白-1基因重组腺病毒载体,转染MSC,探讨LMP-1基因对MSC成骨分化和成骨活性的影响65377; 【方法】 从大鼠成骨细胞内提取总RNA,并设计LMP-1特异性引物进行RT-PCR,获取LMP-1基因后利用Invitrogen公司的TOPO定向克隆技术创建入门克隆pENTR/D-LMP-165377;转化TOP10细菌后,挑取阳性克隆摇菌提取质粒,入门克隆再与表达载体pAD/CMV/V5-DEST进行同源重组反应得到病毒载体pAD/CMV/V5-LMP-1 ...
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Zhongguo shiyan zhenduanxue, 2008 目的建立大鼠骨髓间质干细胞（rat mesenchymal stemcells,rMSCs）体外分离、纯化及扩增的方法及诱导rM-SCs向成骨分化,并进一步研究,提供理想的种子细胞。方法采用贴壁筛选法分离rMSCs,并通过不断传代进行纯化和扩增培养,检测rMSCs生长周期,绘制细胞生长曲线。检测碱性磷酸酶活性,钙结节的形成。结果rMSCs在体外分离培养扩增,rMSCs形态呈长梭形,细胞周期显示第3代MSCs约有81.48%的细胞处于G0/G1期,细胞生长曲线呈S形。经地塞米松、β-甘油磷酸钠 ...
罗宗键 +4 more
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Zhongguo shiyan zhenduanxue, 2013 间充质干细胞（Mesenchymal stem cells,MSCs）是一种具有多向分化潜能的干细胞,研究报道从骨髓、胎盘组织、肌肉组织、脂肪组织、脐带血中均可分离到MSCs[1-3],在适当培养条件MSCs下可分化为成骨细胞、软骨细胞和肌肉细胞神经元、神经胶质细胞和肝细胞等[4-9],与其他种类干细胞相比,MSCs来源丰富、体外易于分离培养、免疫源性低,已成为干细胞移植领域较为理想的种子细胞 ...
赵洪昌, 王莹, 王峰
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[Three-dimensional printed scaffolds with sodium alginate/chitosan/mineralized collagen for promoting osteogenic differentiation]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za ZhiYang B +6 more
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[Knockdown of NPTX1 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanShuai T, Guo Y, Lin C, Hou X, Jin C.
europepmc +1 more source
SPIO 标记下大鼠骨髓间充质干细胞生物学特性及 多向分化潜能及体外MR成像
Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 探讨超顺磁氧化铁纳米颗粒(SPIO)标记对骨髓间充质干细胞生物学特性和多向分化潜能的影响以及体外磁共振(MRI)成像的可行性,为移植干细胞活体MRI成像提供实验基础65377; 【方法】 采用梯度密度离心法和贴壁法获取大鼠骨髓间充质干细胞(BMSC),采用25 μg/mL SPIO联合0.75 μg/mL多聚赖氨酸(PLL)标记BMSC,比较标记和未标记MSC细胞活力65380;增值65380;细胞周期65380;凋亡和成骨65380;成脂多向分化能力;应用1.5T ...
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[Mechanism analysis of ω-3 polyunsaturated fatty acids in alleviating oxidative stress and promoting osteogenic differentiation of MC3T3-E1 cells through activating Nrf2/NQO1 pathway]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiHuang J +5 more
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[Effect of astragaloside IV on osteogenic differentiation of BMSCs in osteoporotic rats via regulation of miR-21 and inhibition of the Notch signaling pathway]. [PDF]
Zhong Nan Da Xue Xue Bao Yi Xue BanXiao J, Liu X, Huang J, Dou B.
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[Effects and mechanism of morroniside on osteogenic differentiation and proliferation of mouse MC3T3-E1 cells]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, 2022 Dong R +5 more
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[Hydrodynamic finite element analysis of biological scaffolds with different pore sizes for cell growth and osteogenic differentiation]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanHu Y, Lyu W, Xia W, Liu Y.
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