Results 41 to 50 of about 11,811 (167)
Equine Veterinary Journal, Volume 57, Issue 1, Page 87-100, January 2025.Abstract Background Radiological findings in the proximal sesamoid bones (sesamoids) are a persistent source of controversy at Thoroughbred sales, due to inconsistent classification and conflicting assignment of potential clinical importance. Objectives To define the prevalence of sesamoid findings on sales repository radiographs in yearling and 2‐year‐
Frances J. Peat +5 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2006 目的探讨重组人胰岛素生长因子（rhIGF-1）基因对体外培养大鼠成骨细胞增殖分化的影响。方法采用酶消化法获取大鼠乳鼠颅骨成骨细胞,传至第3代进行成骨细胞鉴定,钙化结节用茜素红（ARS）染色,钙钴法显示成骨细胞中碱性磷酸酶（ALP）的表达。用脂质体法将pcDNA3.1-rhIGF-1质粒转染成骨细胞。倒置显微镜下观察细胞的生长形态,MTT法检测成骨细胞增殖情况,碱性磷酸酶及骨钙素（OCN）测定成骨细胞活性。结果建立了稳定的大鼠成骨细胞模型;pcDNA3.1-rhIGF ...
李辉南, 高凤彤, 李剑威, 王华
doaj
Investigation on bone cell-biomaterial interaction by biochip techniques and in-situ physicochemical methods [PDF]
, 2012 现代微系统技术及各种原位物理化学方法，为生物表面/界面的研究提供了前所未有的机遇和全新的研究层次。本工作致力于应用发展的微流控和微电极阵列（MEA）生物芯片、原子力显微镜（AFM）、电化学阻抗谱（EIS）和衰减全反射傅立叶红外光谱（ATR-FTIR）等先进技术，从不同的侧面和层次研究骨细胞与生物材料相互作用过程及机制。 主要研究内容有：（a）建立昆明小鼠的原代颅骨成骨细胞和长骨骨髓基质干细胞（rMSCs）的培养，并应用于新型复合生物材料的研究；（b）考察微流控生物芯片中流体状态（静止和流动 ...
张帆
core
Advances in nanomaterials for promoting bone tissue regeneration by reducing reactive oxygen species levels [PDF]
Reactive oxygen species (ROS) are common products of bone tissue injury. If ROS cannot be removed in time, oxidative stress will be induced in the cells, which will have a negative effect on the regeneration of bone tissue.
GUO Shangchun +3 more
core +1 more source
Ultrasound in Obstetrics &Gynecology, Volume 64, Issue 5, Page 661-668, November 2024.ABSTRACT Objective To determine the diagnostic accuracy of virtual autopsy using whole‐body postmortem ultra‐high field magnetic resonance imaging (MRI) at 7 Tesla (T), using a short T2‐weighted imaging (T2‐WI) protocol, compared with classical autopsy, for detecting structural abnormalities in small second‐trimester fetuses. Methods Thirty consecutive
A. Staicu +11 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2002 【目的】探讨不同剂量电离辐射后大鼠类成骨细胞生物学性状的改变。【方法】成骨细胞通过酶消化法从SD 大 鼠乳鼠颅骨中获取传代;将第2 代细胞分别进行0、1、4 、6 、9 Gy 的γ线辐射, 检测成骨细胞的增殖、碱性磷酸酶活性和骨钙素 分泌量。【结果】细胞增殖、功能和分化受γ线电离辐射抑制的程度不同, 1 Gy 时增殖即受到抑制, 骨钙素分泌量在4 Gy 时才 受到显著地抑制, 而碱性磷酸酶活性值甚至在6 Gy 下抑制作用才有意义。【结论】电离辐射后, 成骨细胞的增殖 ...
王剑宁
doaj
Zhejiang Daxue xuebao. Lixue ban, 2008 探讨了中药地龙水提液和醇提液有无促进成骨细胞增殖、分化和基质矿化的作用及对大鼠牙槽骨吸收疗效进行了研究.制备地龙的水相及醇相提取液，以小鼠成骨细胞株MC3T3-E1作为体外药效的试验模型，分别通过MTT法.流式细胞术，碱性磷酸酶活性测定，骨钙素检测和Von kossa基质矿化染色法观察上述药物的促细胞增殖、分化、基质矿化作用.以实验牙位龈正中注射脂多糖建立的SD大鼠实验性牙槽骨吸收作为体内药效试验模型，通过组织切片形态学骨密度观察和抗酒石酸酸性磷酸酶染色评价上述药物对大鼠实验性牙槽骨吸收的疗效.结果发现：
DINGPei-hui(丁佩惠) +3 more
doaj +1 more source
Engineering of mesenchymal stem cell-derived exosomes and their application progress in the field of oral medicine [PDF]
In recent years, mesenchymal stem cell-derived exosomes (MSC-EXO) have garnered increasing attention in the field of stomatology and have become an established research area in biomedical research. This article reviews the engineering of exosomes derived
ZHAO Yunfeng, LIU Qian, LI Meng, LI Luying, ZHANG Wei, HU Xiantong, MA Chufan
core +1 more source
, 2020 【目的】本研究旨在探讨 INI1 在上皮样肉瘤细胞中作用及机制。【方法】利用 Western blot 检测并比较上皮样肉瘤细胞中INI1 表达水平；免疫组化检测上皮样肉瘤组织标本中INI1 的表达；利用Tet-on 系统重建上皮样肉瘤细胞VA-ES-BJ 中INI1 表达；诱导VA-ES-BJ 细胞中INI1 表达后检测细胞形态、增殖、脂肪相关分子标志，进一步Western blot 检测脂肪分化相关转录因子表达及油红染色检测向脂肪方向分化的程度。【结果】在上皮样肉瘤细胞和临床标本中均存在INI1 ...
吕东明 +5 more
core
Study on the gene expression profile in the bone tissue in primary osteoporosis with kidney-yang deficiency syndrome [PDF]
, 2017 目的通过检测原发性骨质疏松症不同中医证型患者骨组织基因表达谱的差异,探讨原发性骨质疏松症肾阳虚证相关基因的信息学特征。方法随机选择原发性骨质疏松; 症患者,中医辨证分型为肾阳虚证组3例,肾阴虚证组3例,无肾虚证组3例,并选择正常骨密度人群3例设为正常对照组。用人全基因组表达谱芯片检测4组人群; 骨组织基因表达谱,筛选共同的差异表达基因,并对这些差异表达基因进行基因通路等相关功能分析。结果肾阳虚证组与正常对照组、肾阴虚证组、无肾虚证组的差; 异表达基因分别为2631条、3976条、6184条 ...
冯尔宥 +6 more
core +1 more source