Results 11 to 20 of about 1,399 (81)

Effects of bone marrow-derived mesenchymal stem cell layer on expansion and differentiation of hematopoietic stem cells from cord blood(骨髓间充质干细胞与造血干细胞共培养对脐血造血干细胞扩增的作用)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2003
脐带血造血干细胞的异体移植对高剂量化疗的患者恢复造血功能是一种有效的细胞学疗法.脐带血造血干细胞的体外扩增能提高有效植入的造血干/祖细胞的数量.将扩增10 d的造血干/祖细胞移植给高剂量化疗的成人患者,患者平均约在移植后的第26天(第15~45天)达到了嗜中性粒细胞的植入.为了加快有核细胞数量的扩增和嗜中性粒细胞的成熟,在以骨髓间充质干细胞为培养基质细胞层,采取阶段扩增方法培养单个核细胞和分选的CD34+细胞,并与无骨髓间充质干细胞的培养体系进行对照.从冻存的脐带血中分离的单个核细胞和CD34 ...
WANGJin-fu(王金福)   +3 more
doaj   +1 more source

From Microstructure to Macroscopic Performance: A Comprehensive Study on Low‐K Oxide‐Doped BNT–LST–NN Ceramics for Energy Storage

open access: yesRare Metals, EarlyView.
ABSTRACT Dielectric ceramics are popular for energy storage materials because of their rapid charge–discharge characteristics, whereas their low energy density and efficiency hinder their miniaturization and integration. In this work, the energy storage performance is significantly enhanced through introducing Al2O3 and TiO2 into the 0.425(Bi0.5Na0.5 ...
Qiansi Zhang   +9 more
wiley   +1 more source

Surface‐Embedded Ag@Multilayer Graphene for Photothermal‐Enhanced Superhydrophobic Anti‐Icing Coatings

open access: yesRare Metals, EarlyView.
ABSTRACT Harnessing solar energy for self‐heating presents an effective strategy to suppress surface ice formation. In this paper, we fabricated nano‐silver‐modified multilayer graphene sheets (Ag@MGs) that integrate photothermal and superhydrophobic properties for anti‐icing applications.
Yihan Zhang   +7 more
wiley   +1 more source

含脐血MSCs 体系扩增后的脐血重建 NOD/SCID小鼠造血的研究

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2005
【目的】探讨含人脐血(umbilical cord blood,UCB)来源的间充质干细胞(mesenchymal stem cells,MSCs)联合造血生长因子(hematopoietic growth factors,HGFs)体系扩增后的脐血造血细胞在NOD/SCID小鼠体内植入及重建小鼠造血的能力。【方法】①用含女性胎儿脐血MSCs(UCBMSCs)及HGFs组合的无血清扩增体系体外扩增男性新生儿UCB CD34~+细胞。②收获扩增第6天的UCB细胞,经尾静脉移植给亚致死量照射后的雌性NOD ...
周敦华   +8 more
doaj  

Hierarchical Strengthening Design in AlCrCuFeNi2.5/CuCrZr Composites Enables Strength–Ductility–Conductivity Synergy

open access: yesRare Metals, EarlyView.
ABSTRACT Developing Cu‐based composites that simultaneously achieve ultra‐high strength, ductility, and electrical conductivity remains critical for advanced electrical contact applications. In this work, multiphase AlCrCuFeNi2.5 high‐entropy alloy (HEA) reinforcements are incorporated into CuCrZr matrix via spark plasma sintering (SPS) and annealing ...
Tao Hong   +8 more
wiley   +1 more source

标本扩增效率的计算及其对HBV DNA定量结果的影响

open access: yesZhongguo shiyan zhenduanxue, 2006
目的建立分析标本扩增效率的方法,分析其对HBV DNA定量结果的影响。方法应用分析软件提供的样点拟合法及对数荧光-循环数坐标图,计算得到Ct,HBV DNA拷贝数以及每一标本的扩增效率。分析40个HBVDNA拷贝数在104108/ml的标本的扩增效率,并用得到的标本实际扩增效率加以校正。结果4个标准的扩增效率分别为0.80,0.80,0.84和0.85。标本的扩增效率在0.590.85之间,其中30份(75%)在0.70.85之间,8份(20%)在0.650.70之间,其余2份在0.65以下 ...
陈英剑, 胡成进, 克丙申
doaj  

On the Damage Tolerance of Diffusion Bonded Titanium–Aluminum Phase Biomimetic Composites

open access: yesRare Metals, EarlyView.
ABSTRACT The biomimetic design principles of natural structures involve the strategic integration of stiff and compliant phases to synergistically enhance strength and toughness. Its evolutionary refined natural armor, characterized by a specialized microarchitecture, enables superior damage tolerance.
Shuai Tong   +10 more
wiley   +1 more source

毛囊COL4A5基因扩增的方法学研究

open access: yesZhongguo shiyan zhenduanxue, 2007
目的探讨毛囊基因组在COL4A5基因扩增中的应用。方法对32例慢性肾病患者和12例健康体检者进行毛囊和全血COL4A5基因扩增,产物测序,比较两者扩增产物,并将测序结果与NCBI公布的标准序列进行比对。结果毛囊与全血COL4A5基因扩增产物长短一致,测序结果与NCBI公布的COL4A5基因标准序列完全相符,且扩增效率无显著性差异(P>0.05)。结论毛囊COL4A5基因扩增效果与全血等同。
刘奇才   +3 more
doaj  

Changes of Potential Suitable Areas for Lynx Under Climate Change in Mohe Area, Daxing'anling Mountains, China

open access: yesWildlife Letters, EarlyView.
Climate change drives shifts in suitable habitats for Eurasian lynx and its prey (hare, roe deer) in Mohe, Daxing'anling Mountains. Under RCP scenarios, moderate warming (RCP4.5) promotes substantial habitat expansion, while high‐emission conditions (RCP8.5) lead to strong expansion in the 2050s but slower gains and partial contraction by the 2070s ...
Binglian Liu   +5 more
wiley   +1 more source

表达降钙素基因相关肽的单纯疱疹病毒Ⅰ型扩增子载体的构建、包装和扩增

open access: yesZhongguo shiyan zhenduanxue, 2006
目的对单纯疱疹病毒Ⅰ型扩增子质料装载降钙素基因相关肽,并进行包装和扩增。方法分别钓取pac基因和γ134.5基因,克隆入pMD18-T载体中。pSV载体经HindⅢ和BamHⅠ双酶切,获得lacZ片段,自身环化形成pUPO-γ134.5-lacZ。合成CGRP基因序列,将此基因替换pUPO-γ134.5-lacZ质粒中的lacZ基因,插入γ134.5基因位点中,即pUPO-Δγ134.5-CGRP。再用单纯疱疹病毒温敏株辅助在BHK细胞中包装、扩增,通过检测lacZ的表达反映扩增子病毒滴度 ...
吴军   +5 more
doaj  

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