Results 11 to 20 of about 16,260 (175)
利用改良Nest-tetra-primerspecificPCR技术对两种石斑鱼的鉴别 [PDF]
, 2009 云纹石斑鱼(EPInEPHEluS MOArA)和褐石斑鱼(E.brunEuS)为石斑鱼属内近缘种,外形相似常被混淆.本研究改良建立了nEST-TETrA-PrIMEr SPECIfIC PCr方法,获得了云纹石斑鱼和褐石斑鱼线粒体dnAnd2基因内的3个特异性条带,分别为内参序列nC1(394bP)、特异性条带nd2-M(268bP)和nd2-b(122bP),以及核基因组中核糖体dnAITS1区的5个特异性条带,分别为内参序列nC2(588bP)、nC3(563bP),特异性条带rdnA-M ...
丁少雄 +4 more
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Impacts of Human Development on Wildlife Use of Corridors in Botswana 人类开发对博茨瓦纳野生动物廊道利用的影响
Integrative Conservation, EarlyView.This study examined the effect of landscape‐scale human impact on wildlife in Botswana. We examined how species use wildlife corridors in two different human‐dominated landscapes. We identified differences in temporal overlap between wildlife and humans across both landscapes, which is fundamental to consider in both current and future conservation ...
Tempe S. F. Adams +3 more
wiley +1 more source
Wildlife Letters, EarlyView.Climate change drives shifts in suitable habitats for Eurasian lynx and its prey (hare, roe deer) in Mohe, Daxing'anling Mountains. Under RCP scenarios, moderate warming (RCP4.5) promotes substantial habitat expansion, while high‐emission conditions (RCP8.5) lead to strong expansion in the 2050s but slower gains and partial contraction by the 2070s ...
Binglian Liu +5 more
wiley +1 more source
New Devices for Droplet Generation and Their Application in Single-Molecule Amplification and Single Cell Manipulation [PDF]
, 2016 单分子、单细胞是生物体系的最基本单位。分子之间的不同决定了其引发反应的不同，细胞之间的不同决定了生命活动的不同。因此，深入研究单分子和单细胞对人们探索生命活动本质是十分必要的。与传统研究对象不同，单个分子、单个细胞的研究需要更加精准的研究平台，从而避免大背景的干扰，提高信噪比。微流控芯片，是一种将常规反应小型化、集成化新型的技术。其具有反应迅速、反应体积小、信噪比高、通量高、结果精准、试剂节约等特点，这些优点十分符合目前对单分子、单细胞研究的要求。因此在近些年来，微流控芯片技术在单分子 ...
李星锐
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Conservation Biology, EarlyView.Scaling area‐based conservation, including initiatives led or comanaged by Indigenous Peoples and local communities, is a flagship goal of the Kunming‐Montreal Global Biodiversity Framework. Conservationists often aspire to scale initiatives, but this is rarely achieved in practice. Identifying and addressing factors that limit initiative adoption (i.e.
Thomas Pienkowski +39 more
wiley +1 more source
The effect of graphene-based derivatives on polymerase chain reaction [PDF]
, 2015 实际操作中，聚合酶链式反应（PCR）存在某些局限性，如重复性差、扩增效率低等问题。为此研究人员进行了积极的探索和改进，尝试将纳米材料引入PCR体系进行优化，并取得了阶段性成果。石墨烯作为一种新兴纳米材料，具有特殊的表面效应和尺寸效应，能够进行多种修饰，易与生物大分子蛋白质、核酸等相互作用，对提高PCR扩增特异性和效率都有明显的效果，具有非常重要的理论意义和应用价值。本文制备了不同尺寸及功能化的石墨烯，研究其作为添加剂对PCR的影响，并探讨其潜在的影响机制，主要研究结果如下： 1.
熊云景
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Conservation Biology, EarlyView.Abstract Illegal wildlife trade (IWT) is one of the largest global illegal activities, and it negatively affects biodiversity and sustainable development worldwide. DNA barcoding coupled with high‐throughput sequencing (i.e., metabarcoding) is useful in identifying taxa affected by IWT and has been used routinely for decades.
Maria Joana Ferreira da Silva +9 more
wiley +1 more source
污水处理厂中浮游生物群落DNA指纹及其与水质指标的关系 [PDF]
, 2008 以武汉龙王嘴污水处理系统为研究对象,揭示了污水处理各阶段中浮游生物群落的DNA指纹拓扑结构,进而探索了其与浮游生物群落结构和环境理化因子的关系.首先建立了污水处理系统中浮游生物群落总DNA提取方法,然后用原核与真核特异性引物对流程(A2/O氧化沟工艺)中不同阶段的浮游生物群落总DNA进行PCR扩增,用变性梯度凝胶电泳(DGGE)检测,并对平行水样分别进行常规理化因子和浮游生物物种的检测与鉴定.结果显示,各采样点理化因子、物种组成与浮游生物DNA指纹的统计分析结果十分吻合,厌氧、缺氧和好氧阶段间差异较小,
余育和 +4 more
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Hobbyist preferences for pet freshwater turtles
Conservation Biology, EarlyView.Abstract The burgeoning pet trade is a primary threat to wild freshwater turtles worldwide. Although the risks from commercial exploitation of turtles have been discussed widely, there is little empirical research on preferences for pet turtles from a hobbyists’ perspective.
Jingjing Zhao +7 more
wiley +1 more source
Population genetic structure and phylogentic analysis for Morone americana in Delaware Bay of United States [PDF]
, 2016 美洲狼鲈(Moroneamericana，Gmelin，1789)隶属于鲈形目(Perciformes)，狼鲈科(Moronidae)，狼鲈属(Morone)，别名美洲白鲈、白石鮨。美洲狼鲈是在美国东海岸浅海区域十分常见的一种溯河洄游鱼类，每逢春末夏初，生活于近海和河口区域的美洲狼鲈便溯河洄游至河流中上游产卵繁衍。美洲狼鲈也经常被用作海区污染程度的指示种。鉴于美洲狼鲈广泛的分布范围以及巨大的种群数量，其十分适合作为近岸洄游性鱼类群体研究的模式种类。同时，其在检测环境污染方面也具有主要作用 ...
边力
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