Results 21 to 30 of about 16,260 (175)
In vitro culture, cryopreservation, and field reintroduction of the endangered Mingan thistle
Conservation Biology, EarlyView.Abstract Current plant conservation efforts are hindered by, for example, poor seed germination, low viability, and insufficient propagation and preservation technologies. To address these problems, we devised an approach to plant conservation that integrates conservation, preservation, and restoration (CPR), which uses advanced in vitro techniques. We
Mukund R. Shukla +4 more
wiley +1 more source
Characterization and function analysis of DNA replication enzymes from Hyperthermophilic archaea Palaeococcus pacificus DY20341 [PDF]
, 2016 DNA是至今发现的绝大多数生物遗传信息的携带者。DNA复制机制保证了细胞分裂期间遗传信息的复制和传递，是维持生物遗传稳定性的前提。DNA复制过程涉及几十种蛋白和酶的参与以确保遗传信息被准确而及时的复制。 研究DNA复制对人类疾病，特别是肿瘤及传染病的机理探索有至关重要的意义。然而参与真核生物(包括人类)DNA复制的酶系非常复杂，对真核生物DNA复制机制的研究面临着诸多挑战。细菌的DNA复制系统相对简单，但其复制酶系与真核生物差别较大。古菌的复制酶系与真核生物的复制酶系相似，而且相对简单 ...
赵文昭
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Effects of parachute science on local research capacity 降落伞科学对当地研究能力的影响
Conservation Biology, EarlyView.Abstract Strengthening research capacity is essential to address the global biodiversity crisis. Yet, parachute science often undermines this goal, and its prevalence, costs, and benefits are unclear. We analyzed 13,502 publications on primate research that we extracted from Scopus (1960–2022) to evaluate the effects of parachute science on local ...
Li Yang +11 more
wiley +1 more source
, 2012 利用泥鳅(Misgurnus anguillicaudatus)的微卫星引物跨种扩增大鳞副泥鳅(Paramisgurnus dabryanus),通过家系扩增、PCR产物测序筛选出6个座位,用于大鳞副泥鳅7个野生群体的遗传结构分析。结果显示,群体的平均等位基因数在3.167～4.833,平均观测杂合度(Ho)在0.248～0.417,平均期望杂合度(He)在0.379～0.505,多个座位存在零等位基因、杂合子缺失现象,显示大鳞副泥鳅种群遗传多样性较低。采用UPGMA法对7个群体基于DA遗传距离进行聚类,
俞小牧, 游翠红, 童金苟
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The collective application of shorebird tracking data to conservation
Conservation Biology, EarlyView.Abstract Addressing urgent conservation issues, such as the drastic declines of North American migratory birds, requires creative, evidence‐based, efficient, and collaborative approaches. The abundance of over 50% of monitored North American shorebird populations has declined by over 50% since 1980. To address these declines, we developed a partnership
Autumn‐Lynn Harrison +71 more
wiley +1 more source
Diagnotic Value of the Combined Determination of Telomerase Activity in Induced Sputum, Pleural Effusion and Fiberobronchoscopic Biopsy Samples in Lung Cancer [PDF]
, 2010 Background and objective It has been proven that telomerase activation correlates with the carcinogenesis, aggressiveness and turnover of lung cancer.
Cheng ZHAO +4 more
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Conservation Biology, EarlyView.Abstract Human development is a driver of global change and a major threat to biodiversity. Protected areas maintain and support biodiversity, but outside stressors, such as climate change and land use change, can negatively influence natural resources within protected areas.
Leah J. Rudge +2 more
wiley +1 more source
表达降钙素基因相关肽的单纯疱疹病毒Ⅰ型扩增子载体的构建、包装和扩增
Zhongguo shiyan zhenduanxue, 2006 目的对单纯疱疹病毒Ⅰ型扩增子质料装载降钙素基因相关肽,并进行包装和扩增。方法分别钓取pac基因和γ134.5基因,克隆入pMD18-T载体中。pSV载体经HindⅢ和BamHⅠ双酶切,获得lacZ片段,自身环化形成pUPO-γ134.5-lacZ。合成CGRP基因序列,将此基因替换pUPO-γ134.5-lacZ质粒中的lacZ基因,插入γ134.5基因位点中,即pUPO-Δγ134.5-CGRP。再用单纯疱疹病毒温敏株辅助在BHK细胞中包装、扩增,通过检测lacZ的表达反映扩增子病毒滴度 ...
吴军 +5 more
doaj
, 2010 果糖-1,6-二磷酸酶(EC 3.1.3.11)是糖异生中的关键限速酶之一,在糖代谢中起重要作用。哺乳动物存在肝脏型和肌肉型两种果糖-1,6-二磷酸酶同工酶,分别由Fbp1和Fbp2编码。银鲫作为我国重要的经济养殖鱼类,尚无果糖-1,6-二磷酸酶基因的有关资料,其组织分布特征和胚胎发育模式亦不清楚。本研究采用RACE方法从银鲫原肠胚SMART cDNA文库中扩增了果糖-1,6-二磷酸酶基因的全长cDNA,其长度为1170 bp,编码337个氨基酸残基,多重序列比对和系统发育分析表明该基因为肝脏型果糖 ...
桂建芳, 王锐, 肖青
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Harnessing social media data to track species range shifts
Conservation Biology, EarlyView.Abstract Biodiversity monitoring programs and citizen science data remain heavily biased toward the Global North. Especially in megadiverse countries with limited biodiversity records, incorporating social media data can help address existing data gaps.
Shawan Chowdhury +15 more
wiley +1 more source