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拟南芥 - Open Access .click
Results 1 to 10 of about 101 (97)

SWEET1-mediated glucose transport is crucial for energy availability in Arabidopsis. [PDF]

open access: yesNew Phytol
Summary The stress‐responsive hormone abscisic acid (ABA) is known for its inhibitory effects on various physiological processes, including seed germination, often resulting in energy deprivation. Interestingly, ABA‐induced germination inhibition can be alleviated by exogenous glucose (Glc), mimicking a functional interplay between ABA and sugar ...
Xue X, Li J, Yu YC, Chen LQ.
europepmc   +2 more sources

Guard-cell expression of abscisic acid receptors for engineering water-use-efficient plants without trade-offs in growth. [PDF]

open access: yesNew Phytol
Summary Abscisic acid (ABA) induces stomatal closure, reducing transpirational water loss, a critical adaptation for agriculture under drought. However, ABA is often viewed cautiously as stomatal closure limits CO2 uptake for photosynthesis and growth.
Liu J   +4 more
europepmc   +2 more sources

MsaH2A.W is identified response to salt tolerance in Miscanthus sacchariflorus

open access: yesGCB Bioenergy, Volume 15, Issue 8, Page 1058-1073, August 2023., 2023
To study the underlying genetic basis of Miscanthus adaptation to salt stress, the transcriptome variations of Miscanthus sinensis, Miscanthus sacchariflorus and their hybrid were analysed using RNA‐seq technology under salt treatment. According to the expression pattern of known salt‐tolerant genes, a histone variant gene MsaH2AW of M.
Ting Yu   +12 more
wiley   +1 more source

油菜BnRCH基因提高转基因拟南芥的耐盐性研究

open access: yes四川大学学报. 自然科学版, 2013
为探索本实验室从甘蓝型油菜中克隆到的BnRCH基因在植物耐盐中的作用,比较了NaCl胁迫下转基因与野生型拟南芥在萌发及幼苗生长的差异.结果表明:在100 mMNaCl处理下,BnRCH转基因拟南芥种子萌发率比野生型高3~5倍;盐胁迫后野生型拟南芥幼苗首先表现出枯萎、白化现象;除去盐胁迫后,转基因拟南芥幼苗恢复生长状况明显优于野生型.本实验结果表明BnRCH基因能够提高转基因拟南芥的耐盐性.
陈凤莲   +4 more
doaj  

拟南芥中参与盐胁迫应答的基因At1g14260的功能初探

open access: yes四川大学学报. 自然科学版, 2015
拟南芥中未知基因At1g14260的表达受到多种非生物胁迫的诱导,特别是在NaCl的诱导下,At1g14260的表达量明显增加.对T-DNA插入突变体at1g14260(salk-118406)的分析表明,敲除了基因At1g14260的拟南芥相比野生型对盐胁迫更加敏感.此外,构建了融合表达载体PBi221-At1g14260-GFP并且成功转入拟南芥原生质体中,在荧光显微镜下观察到融合蛋白定位于原生质体细胞核中.因此,At1g14260可能参与了拟南芥中盐胁迫的过程.
杨昊   +4 more
doaj  

ABA受体RCAR1/PYL9相互作用蛋白质的筛选及初步研究

open access: yes四川大学学报. 自然科学版, 2011
使用拟南芥作为模型,采用酵母双杂交系统筛选出和ABA受体RCAR1/PYL9有相互作用的蛋白质,以更深入的研究RCAR1/PYL9的功能.首先利用反转录PCR方法获得拟南芥的cDNA,然后通过酵母双杂交系统,用拟南芥RCAR1/PYL9作为诱饵蛋白对拟南芥cDNA文库进行筛选,并获得多个阳性克隆.对该菌落进行进一步的鉴定得到与RCAR1/PYL9具有相互作用的蛋白质,这对进一步研究RCAR1/PYL9及其相互作用蛋白质在ABA信号转导途径中的作用及地位奠定了基础.
李德款   +7 more
doaj  

Fibrillin操纵子在甘蓝型油菜和拟南芥中的瞬时表达

open access: yes四川大学学报. 自然科学版, 2006
将Fibrillin操纵子驱动的荧光素酶报告基因表达载体导入拟南芥原生质体中,研究基因表达调控,证明脱落酸(ABA)诱导报告基因的表达.同时将相同的质粒转化到甘蓝型油菜蜀杂9号原生质体中,获得与拟南芥中类似的表达结果.实验结果说明Fibrillin操纵子在拟南芥和油菜原生质体中均对ABA的诱导敏感.
滕蕾   +4 more
doaj  

AtVDAC2参与拟南芥盐胁迫信号应答过程

open access: yes四川大学学报. 自然科学版, 2012
为了探索VDAC在盐胁迫信号传递途径中可能的传递关系,以AtVDAC2转基因拟南芥过量表达和抑制表达株系为材料,初步探索盐胁迫过程中该基因参与信号传递的方式.实验分析了NaCl处理对种子萌发的影响、Ca2+对NaCl胁迫下种子萌发的影响,以及NaCl对气孔运动的影响和盐胁迫相关基因的表达水平.实验结果指出,AtVDAC2基因表达水平变化影响了拟南芥对NaCl的敏感性:高表达的AtVDAC2使得拟南芥种子对NaCl敏感性提高,种子萌发率降低,气孔关闭较快 ...
文国琴   +4 more
doaj  

甘蓝型油菜BnBRI1基因的克隆及表达分析

open access: yes四川大学学报. 自然科学版, 2014
以拟南芥油菜素内酯受体BRI1基因序列设计同源简并引物,利用同源克隆技术,克隆了甘蓝型油菜中编码油菜素内酯受体基因全长cDNA序列,命名为BnBRI1(GenBank:JX871217),该基因开放阅读框(ORF)大小为3591bp,与拟南芥中BRI1基因(GenBank:NM120100)相似性为94.21%,编码1196个氨基酸,与拟南芥中BRI编码的氨基酸相似性为89.23%.采用实时定量PCR分析表明,BnBRI1基因在根、茎、叶中都有表达,但表达量存在显著差异 ...
刘彩霞, 程文财, 王茂林
doaj  

谷氨酰tRNA合成酶转基因拟南芥的生长特性分析

open access: yes四川大学学报. 自然科学版, 2009
以不同独立株系的拟南芥谷氨酰tRNA合成酶(Arabidopsis glutamyl tRNA syn-thetase,简称AtGluRS)过量表达的三种纯合株系(GS3、GS4、GS6)及抑制表达的三种纯合株系(GAS2、GAS3、GAS7)为实验材料,分析其生长情况和表型。表型分析结果表明,拟南芥谷氨酰tRNA合成酶过量表达植株表现出开花晚、基叶多、茎生长快等与对照和AtGluRS抑制表达植株不同的特点。此外,用氯化钠(NaCl)模拟盐胁迫环境,对转基因植物的萌发率和生长情况进行分析 ...
祝欣   +5 more
doaj  

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