Results 31 to 40 of about 725,502 (174)

Ongoing outbreak of maternal parvovirus B19 infections in Germany since end of 2023: consequence of COVID‐19 pandemic?

open access: yesUltrasound in Obstetrics &Gynecology, Volume 65, Issue 4, Page 456-461, April 2025.
Abstract Objective To investigate the ongoing parvovirus B19 (B19V) outbreak among pregnant women in Germany and its connection to the coronavirus disease 2019 (COVID‐19) pandemic. Methods This retrospective cohort study analyzed anonymous data regarding serologically confirmed B19V infections during pregnancy between January 2014 and April 2024 across
J. Jiménez Cruz   +14 more
wiley   +1 more source

“We are adapting to it because it is within us”: The co‐becoming of COVID‐19 in Malawi

open access: yesWorld Medical &Health Policy, Volume 16, Issue 3, Page 295-315, September 2024.
Abstract Using a case study design, this research explores the Coronavirus 2019 disease (COVID‐19) pandemic from the perspectives and worldviews of Malawians (Black/African knowledge) through the Bawaka Yolŋu ontology of co‐becoming (Black/Indigenous knowledge).
Chúk Odenigbo   +3 more
wiley   +1 more source

A new typology for comparing scientific advisory committees. Evidence from the Italian response to the COVID‐19 pandemic

open access: yesReview of Policy Research, Volume 41, Issue 5, Page 740-769, September 2024.
Abstract The study of scientific advisory committees (SACs) is a recurrent topic of research in public policy and public administration. Scholars are increasingly interested in analyzing the scientization of policy advice as well as the role played by knowledge‐based policymaking processes.
Giliberto Capano   +4 more
wiley   +1 more source

Reframing wildlife disease management problems with decision analysis

open access: yesConservation Biology, Volume 38, Issue 4, August 2024.
Abstract Contemporary wildlife disease management is complex because managers need to respond to a wide range of stakeholders, multiple uncertainties, and difficult trade‐offs that characterize the interconnected challenges of today. Despite general acknowledgment of these complexities, managing wildlife disease tends to be framed as a scientific ...
Margaret C. McEachran   +16 more
wiley   +1 more source

广州地区2460例呼吸道感染患者7种呼吸道病原体RNA检测结果分析

open access: yesZhongguo shiyan zhenduanxue, 2021
目的通过检测7种呼吸道病原体RNA,了解广州地区呼吸道病原体的分布和流行情况。方法采用核酸双扩增法检测2019年3月至2020年2月广东省人民医院2460例呼吸道感染门诊和住院患者咽喉拭子中7种呼吸道病原体RNA,并对患者的性别、年龄、病原体的季节分布等进行统计分析。结果广州地区2640例呼吸道疾病患者7种呼吸道病原体RNA的总阳性率为8.46%(208/2460),阳性率最高的是呼吸道合胞病毒,阳性率为2.85%,其次为肺炎支原体和副流感病毒。女性患者肺炎支原体RNA阳性率明显高于男性患者 ...
刘胜男   +6 more
doaj  

禽流感病毒感染K-562和HL-60肿瘤细胞系的实验研究

open access: yesZhongguo shiyan zhenduanxue, 2013
目的选择代表不同骨髓细胞分化阶段的血液肿瘤细胞株K-562、HL-60作为研究对象,选取低致病性禽流感病毒H3N2/H4N6作为病原体,探讨禽流感病毒感染骨髓的机制。方法应用流式细胞术检测K562等细胞表面禽流感病毒受体;收集感染病毒的细胞培养上清,红细胞凝集实验检测染毒细胞释放的子代病毒;收集感染病毒的细胞免疫荧光检测病毒NP,判断病毒吸附、进入细胞并在细胞内增殖、复制情况。结果所检测细胞表面均有禽流感病毒SAα2,3-Gal和SAα2,6-Gal连接的唾液酸寡糖受体的表达。在H3N2感染的K ...
张红梅   +4 more
doaj  

两株水生呼肠孤病毒部分特性的比较

open access: yes, 2003
水生呼肠孤病毒为感染水生动物的一类病原体,隶属于呼肠孤病毒科新建水生呼肠孤病毒属。草鱼呼肠孤病毒(Grass carp reovirus,GCRV)是引起中国南方淡水养殖草鱼暴发性出血病病原,鲅鱼呼肠孤病毒(Threadfin reovirus,TFV)是引起海水养殖鲅鱼病毒病病原。本研究将GCRV与新加坡TFV分离株进行了部分特性比较研究。结果表明,GCRV与TFV均能感染CIK细胞,但对其它鱼类细胞系的敏感性有所差异。此外,凝胶电泳与逆转录聚合酶链式扩增显示,GCRV与TFV核酸属不同的基因型 ...
汪亚平, 方勤, 丁清泉, 朱作言
core   +2 more sources

长春市2012-2013年流感病毒核酸监测结果分析

open access: yesZhongguo shiyan zhenduanxue, 2013
目的分析长春市流感病毒核酸的监测结果,掌握流感流行规律,为流感防控工作提供科学依据。方法用实时定量PCR法对采集的流感样病例标本进行核酸检测。结果 2012年1月-2013年5月共采集流感样病例标本1037份,总阳性率为25.17%。在此期间,共出现两次流感流行高峰:第一次是在2012年2月-3月,主要以乙型和季节H3N2型流感病毒为主;第二次是在2013年1月,主要以季节H3N2型和新甲型H1N1型流感病毒为主。结论应加强流感监测,避免流感大流行的发生。
崔薇   +6 more
doaj  

用二次PCR方法检测鸟类禽流感的初探

open access: yes野生动物学报, 2022
为进一步提高野生动物疫情监测、病原检测鉴定和应急防控的时效性,建立了对鸟类拭子样品直接进行禽流感病毒(Avian influenza virus,AIV)核酸检测鉴定的二次PCR方法。对比试验结果显示,该方法可行性较高,具有与实时荧光定量PCR(real-time quantitative PCR,qPCR)方法等同的特异性和敏感性。禽流感病毒核酸二次PCR检测方法的建立,有利于基层监测单位在缺乏病毒分离技术和qPCR等实验条件的情况下,利用现有条件,就近、及时地开展野鸟样品的禽流感病毒检测工作。
陈 光 李 勇 朱功良 张 俊 冯 伟 陈 婧彭金山 郑和松 杨国祥
doaj  

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