Verbascoside Inhibits the Activation of NLRP3 Inflammasome and Improves BV-2 Cell Inflammatory Damage [PDF]
Objective: To investigate the main component verbascoside (VB) in Osmanthus fragrans which inhibits NLRP3 inflammasome activation to improve inflammatory damage in BV-2 cells and its related mechanisms.
Fang CHENG +7 more
core +1 more source
Pifu-xingbing zhenliaoxue zazhi[目的] 分析神经梅毒患者的脑脊液代谢物，以确定潜在的诊断标志物。 [方法] 利用液相色谱-质谱联用技术的非靶向代谢组学，分析5例神经梅毒患者和5例梅毒/非神经梅毒患者的脑脊液样本。将原始数据经过质量控制和归一化处理后，进行数据分析（主成分分析、正交偏最小二乘判别分析、层次聚类分析）确定两组间的差异代谢物。 [结果] 神经梅毒患者体内，有6种代谢物显著升高，分别是Pro-leu、nudifloramide、N-oleoyl glycine、DMP 777 ...
贺 媛媛 +5 more
doaj +1 more source
Zhongguo shiyan zhenduanxue, 2013 目的观察α-enolase在大肠癌中的表达情况及意义,探讨其与大肠癌发生发展及转移的关系。方法采用液质联用技术对18例大肠癌患者的癌组织和癌旁组织进行蛋白质谱的检测、鉴定和分析,并通过免疫蛋白印迹实验进一步确证。结果α-enolase在大肠癌组织中表达高于癌旁正常组织。结论高表达的α-enolase可能与大肠癌的发生发展有关。
王庭利 +6 more
doaj
Establishment of monoclonal antibody-based immunoassay of two bio-active components from some compound prescriptions of traditional Chinese medicines [PDF]
, 2008 通过杂交瘤技术制备中药主要生物活性成分的单克隆抗体，利用单克隆抗体对中药成分进行测定等应用研究，是一种很有意义的研究中药的方法。这种方法拥有免疫反应的高特异性、高灵敏度、方便快捷等优点。 本研究将复方中药“芍药甘草汤”中主要成分芍药苷和甘草酸分别与蛋白载体连接，制成人工抗原。将合成的抗原免疫Balb/c小鼠，然后以杂交瘤技术制备单克隆抗体，并通过小鼠腹水大量生产抗体。得到的单抗经初步鉴定，证明其具有高效价、高敏感性以及高特异性。利用这两种单克隆抗体建立了竞争性ELISA方法 ...
林纪昀
core
Optimisation of the Extraction Process and Compositional Analysis of Lipase-inhibiting Components from Morus alba L. Leaves [PDF]
In this study, on the basis of single factor experiments, the effects of raw material mass concentration, ethanol volume fraction, ultrasonic power, extraction temperature, and extraction time on the lipase inhibition activity of mulberry leaves extract ...
Daorui PANG +6 more
core +1 more source
Zhongguo shiyan zhenduanxue, 2011 目的探讨卵巢浆液性囊腺癌与卵巢浆液性囊腺瘤的差异表达蛋白质谱,从而寻找特异性诊断标志物。方法采用二维液质联用分析技术。结果卵巢浆液性囊腺癌与卵巢浆液性囊腺瘤两组间差异蛋白数共计128个。其中116个蛋白在卵巢浆液性囊腺癌中表达上调,12个蛋白表达下调。结论纤维结合蛋白1,基膜聚糖,核心蛋白多糖,骨诱导因子,微管蛋白A、B,肌动蛋白,肌动蛋白素,角蛋白8,结蛋白,膜联蛋白A1、A2,YWHAZ,踝蛋白1,转酮醇酶1,组织蛋白酶D,X线交错互补修复基因6,谷胱甘肽转移酶,核仁蛋白,抑制素,抗原提呈蛋白1 ...
刘彦红 +4 more
doaj
NMR联用HILIC-UPLC-MS/MS测定TNBS诱导的结肠炎大鼠尿液和血浆代谢物变化
Zhongshan Daxue xuebao. Yixue kexue ban, 2021 目的基于非靶标和靶标代谢组学技术，探讨三硝基苯磺酸（TNBS）诱导的结肠炎模型大鼠尿液以及血浆中内源性代谢物的变化。方法16只雄性SD大鼠随机分成对照组和模型组，每组8只，对模型组大鼠给予TNBS灌肠以建立实验性大鼠结肠炎模型。利用核磁共振氢谱（1H NMR）检测两组大鼠尿液代谢物，同时联用HILIC模式超高效液相色谱串联三重四级杆质谱（HILIC-UPLC-MS/MS）测定以上大鼠尿液以及血浆中氨基酸水平，并通过多元变量统计方法对代谢组学数据进行分析。结果同对照组相比，1H ...
刘雯 +7 more
doaj
Effect of Nanofiltration Membrane on Volatile Components in Extraction Solution of Cymbopogon citratus(纳滤膜对香茅提取液中挥发性成分的影响) [PDF]
Shipin kexue jishu xuebaoThe effects of different pore sizes nanofiltration membrane on the volatile components and contents of Cymbopogon citratus extract were analyzed, which provided a technical reference for the preparation of natural flavors in Cymbopogon citratus.
LIU Yuesen(刘月森) +9 more
doaj +1 more source
Identification of Polyphenolic Components in Gastrodia elata and Their Inhibitory Effects on α-Amylase and α-Glucosidase [PDF]
To investigate the composition and hypoglycemic activity of polyphenols in Gastrodia elata, this study utilized ultra-high performance liquid chromatography coupled with quadrupole and electrostatic field Orbitrap high-resolution mass spectrometry (UPLC ...
DONG Shihao, XU Ningmeng, FU Zhengjian, SHEN Kaize, ZENG Shunchao, FAN Fangyu, GUO Lei
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Preparation of Antioxidant Peptides Derived from Nacre of Nanzhu Shell and Their Inhibitory Activity against Tyrosinase [PDF]
The aim of this study was to prepare antioxidant peptides derived from nacre of Nanzhu shell, evaluate their antioxidant and tyrosinase inhibitory activities, and analyze their amino acid components.
An HE +6 more
core +1 more source