Results 61 to 70 of about 13,129 (162)

Efficacy of hemoperfusion combined with atropine on respiratory failure resulting from organophosphate poisoning and its influences on renal function and neutrophil to lymphocyte ratio

open access: yesLinchuang shenzangbing zazhi, 2020
目的观察血液灌流联合阿托品治疗有机磷中毒导致呼吸衰竭患者的疗效,并研究这一治疗方法对患者肾功能、中性粒细胞/淋巴细胞比值(neutrophil to lymphocyte ratio,NLR)的影响。方法回顾性分析2014年1月至2019年12月在茂名市人民医院就诊的有机磷中毒致呼吸衰竭的患者76例,只采用阿托品治疗的31例记为对照组,采用阿托品联合血液灌流治疗的45例记为观察组。比较两组患者治疗后临床效果及各项临床指标,比较治疗前、后两组患者血清β2微球蛋白(β2-microglobulin,β2 ...
KE Wei-guang   +3 more
doaj  

癌基因Bmi-1引起中心体扩增的可能机制

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2008
[目的] 探讨Bmi-1引起中心体扩增的可能机制。[方法] 体外建立Bmi-1稳定高表达和Bmi-1–RNAi稳定起作用的细胞株, 采用免疫荧光染色法计数中心体(Microtub)异常复制的比率及定位细胞周期素蛋白D1(cyclinD1)和P27的表达,同时进行Western blot检测cyclinD1和p27在核中的表达量。[结果] Microtub染色计数发现与对照细胞相比,中心体异常复制的比率在6-10B/Bmi-1-1细胞中升高,而在CNE2/Bmi-1-RNAi细胞中下降 ...
翁桂香   +7 more
doaj  

Association of ambient air pollutants with blood physiological and biochemical indicators and systemic immune inflammation index in people living around Beijing: an analysis of air pollution and population health data [PDF]

open access: yes
ObjectiveTo investigate the association of ambient air pollutants with blood physiological and biochemical indicators and systemic immune inflammation index in people living around the Beijing metropolitan area and to provide a basis for formulating ...
Bingqian DU   +3 more
core   +1 more source

Evaluation of machine learning prediction of altered inflammatory metabolic state after neoadjuvant therapy for breast cancer [PDF]

open access: yes
Objective·To develop a machine learning approach for early identification of metabolic syndromes associated with inflammatory metabolic state changes in breast cancer patients after neoadjuvant therapy, using common laboratory and transthoracic ...
CHAI Yezi   +7 more
core   +1 more source

槲皮素对骨肉瘤细胞MG-63细胞色素C、Bcl-2及bax表达影响

open access: yesZhongguo shiyan zhenduanxue, 2018
目的探讨槲皮素(Quercetin)对人骨肉瘤细胞系MG-63细胞色素C、Bcl-2及bax表达影响。方法将处于对数生长期的MG-63细胞分为对照组和40μmol/L槲皮素处理组,用二步法RT-qPCR法检测各组细胞Bcl-2和bax表达;Western blot分析Bcl-2、bax及细胞色素C。结果与对照组比较,槲皮素处理组MG-63细胞Bcl-2mRNA及蛋白表达量明显降低,bax mRNA及蛋白表达量明显增加,差异均有统计学意义 ...
张静玲, 王克利, 闫双宝
doaj  

miR-135a靶向调控STAT6对前列腺癌细胞生物学行为的影响

open access: yesZhongguo shiyan zhenduanxue, 2020
目的分析微小RNA-135a(miR-135a)靶向调控信号传导和转录激活因子6(STAT6)对前列腺癌细胞生物学行为的影响。方法选取DU145前列腺癌细胞株,细胞培养后分为空白组、miR-135a阴性对照组、miR-135a转染组,构建miR-135a慢病毒载体,空白组加入常规细胞培养液、生理盐水做空白处理,miR-135a阴性对照组加入miR-135aNC、Lipofectamine 2000试剂行无义序列转染,miR-135a转染组加Hsa-miR-135a、Lipofectamine ...
赵宇明, 张悦, 代亮, 张立民
doaj  

Associations between serum complement component 3a and sC5b⁃9 levels with the prognosis of sepsis patients [PDF]

open access: yes
"Objective To investigate the associations of serum complement component 3a(C3a)and soluble terminalcomplement complex C5b⁃9(sC5b⁃9)levels with the severity of sepsis and 28⁃day clinical prognosis among patients,and to provide new biomarker references ...
SU Yixing,BAO Lei,YU Jun'an,GU Zhijian
core   +2 more sources

低剂量丝裂霉素C对辐射所致染色体损伤的实验观察

open access: yesZhongguo shiyan zhenduanxue, 2007
目的观察低剂量丝裂霉素C(MMC)对辐射损伤小鼠外周血白细胞数变化及染色体畸变的影响。方法小鼠腹腔注射低剂量MMC,再给大剂量X射线照射,或先给大剂量X射线照射再给低剂量MMC。观察小鼠白细胞计数及骨髓细胞染色体畸变。结果所有低剂量MMC处理组小鼠骨髓细胞染色体畸变率均低于单纯大剂量照射组,且低剂量MMC处理组的白细胞数降低程度均较单纯照射组有所减轻。结论低剂量MMC可以减轻辐射所致的染色体损伤,具有辐射防护作用。
高峰   +5 more
doaj  

白细胞VCS参数在儿童支原体和细菌感染中的应用价值

open access: yesZhongguo shiyan zhenduanxue, 2014
目的探讨支原体和细菌感染患儿外周血白细胞VCS参数的变化及临床意义。方法采用Coulter LH750全自动血细胞分析仪,测定120例支原体、细菌感染患儿和60例健康儿童的白细胞VCS参数,并对测定结果进行统计学分析。结果与健康对照组比较,支原体感染组的WBC,MNV,NDW,MNC,MLC,MLS,MMV,MDW和MMC明显增高,而MNS和MMS明显降低 ...
崔颖   +5 more
doaj  

肌动蛋白调节剂在胰腺癌细胞中的表观遗传学作用

open access: yesZhongguo shiyan zhenduanxue, 2020
目的探讨肌动蛋白调节剂(Enabled homolog,ENAH)在胰腺癌(Pancreatic cancer,PC)的表达及其对胰腺癌细胞增殖和凋亡的影响机制。方法实时荧光定量聚合酶链式反应(qRT-PCR)、蛋白免疫印迹法(Western blot)分别检测ENAH在胰腺癌细胞株的表达量及信号通路蛋白验证。小干扰RNA(siRNA)转染的SW1990、CFPAC-1、PANC-1作为实验组,以转染阴性对照(NC)的SW1990、CFPAC-1、PANC-1作为对照组 ...
杨亚利   +4 more
doaj  

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