敲低核蛋白1通过抑制软骨细胞铁死亡延缓骨关节炎病理进展 [PDF]
浙江大学学报. 医学版目的研究核蛋白（Nupr）1调控软骨细胞铁死亡在骨关节炎进展中的作用及机制。方法将小鼠膝关节软骨细胞分为小干扰RNA（siRNA）对照组、靶向Nupr1的siRNA（siNupr1）组、siRNA对照+IL-1β组（siRNA对照干扰24 h后再加入10 ng/mL IL-1β）和siNupr1+IL-1β组（siNupr1干扰24 h后再加入10 ng/mL IL-1β）。采用蛋白质印迹法和定量逆转录聚合酶链反应法分别检测Nupr1蛋白及其信使RNA（mRNA）表达。采用细胞计数试剂盒（CCK ...
廖 太阳 +6 more
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Estrogen upregulates DNA2 expression through the PI3K-AKT pathway in endometrial carcinoma
Journal of Zhejiang University SCIENCE B, 2023 子宫内膜癌是最常见的子宫癌类型,占子宫癌病例的90%。本文通过临床研究,检测子宫内膜癌患者标本中雌激素受体（ER）和DNA复制ATP依赖性解旋酶/核酸酶（DNA2）的表达,并分析两者之间的关系。通过细胞培养实验研究ER调控DNA2表达的机制,证实其与PI3K-AKT通路有关。研究还发现,使用短发夹RNA（shRNA）特异性靶向降低Ishikawa中DNA2的表达,会导致细胞增殖和克隆形成能力降低。总的来说,本研究证实了DNA2作为治疗靶点的可行性,并证明抑制DNA2可使Ishikawa对喜树碱（CPT ...
Xinyan Wang +9 more
semanticscholar +1 more source
PTEN-regulated alternative splicing of FoxM1 affects tumor cell migration [PDF]
, 2023 Objective·To study the effect of phosphatase and tensin homolog on chromosome 10 (PTEN) on alternative splicing of forkhead box M1 (FoxM1), and its impact on tumor cell migration.Methods·PTEN was knocked down by short hairpin RNA (shRNA) in ...
GE Mengkai, SHEN Shaoming, WANG Xiaoling
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Mechanism of breast cancer centrosome regulatory protein SEC23B on tumor invasion and metastasis [PDF]
, 2023 Background and purpose: The novel centrosomal regulatory protein Sec23 homolog B (SEC23B) regulates autophagy in cells, thereby providing energy and nutrients that contribute to tumor growth and distant metastasis.
LENG Jie, QIU Guochun, ZHANG Bo, PU Yan
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Deciphering the suppressive effects of CBX8 on prostate cancer cell invasion [PDF]
, 2023 Objective·To elucidate the regulatory mechanisms of the chromobox protein homolog 8 (CBX8) in prostate cancer metastasis from transcriptome and epigenetic modification perspectives.Methods·The correlation between the expression of CBX proteins and ...
LAO Yimin +3 more
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Effect of DBP inhibition on vascular calcification in chronic uremic rats [PDF]
, 2022 Objective To evaluate the effect of D-site binding protein （DBP） inhibition on vascular calcification in chronic uremic rats. Methods GSE146638 was downloaded from the public database （Gene Expression Omnibus， GEO）， and a total of 10 samples from rat of ...
Cao Sujuan, Liu Shuzheng, Yuan Buqi, Yuan Peile, Zhao Songhe, Zhang Yunfang
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FBXO38 regulates ocular melanoma proliferation through the PI3K-Akt signaling pathway [PDF]
, 2023 Objective·To investigate the effect of F-box only protein 38 (FBXO38) on the ocular melanoma proliferation and the potential regulatory pathway.Methods·Human skin cutaneous melanoma A375 and human uveal melanoma OMM2.3 cell lines with FBXO38 knockdown ...
FAN Xianqun +5 more
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Zhongguo shiyan zhenduanxue, 2023 目的 构建人生长分化因子15(GDF15)低表达肝细胞癌HEPG2细胞系并检测其增殖活力、迁移能力和表型变化。方法 用短发夹RNA(shRNA)构建人肝癌HepG2细胞GDF15稳定低表达细胞系(shGDF15),并用CCK8法检测其增殖活力，用划痕实验和Transwell实验检测其迁移能力，用Western blot法检测E-cadherin和Vimentin表达。结果 shGDF15人肝癌HEPG2细胞GDF15转录和翻译水平均显明低于常规培养的HEPG2;shGDF15人肝癌HEPG2细胞24 ...
艾郁葱, 闫光志
doaj
Pyruvate kinase M2 inhibits cisplatin induced caspase-dependent apoptosis via ERK-mediated signaling pathway in hepatocellular carcinoma cells [PDF]
, 2016 M2型丙酮酸激酶（pyruvatekinaseisoenzymetypeM2，PKM2）在肿瘤代谢 和生长等方面发挥重要作用。此外，PKM2还可作为蛋白激酶及辅助转录因子， 调控相关基因的转录表达，促进肿瘤细胞的增殖。我们实验室前期已经证实了 PKM2在肝细胞癌中高表达，并通过EGF/EGFR及TGFβ/TGFβR信号通路调控 肝癌细胞的迁移、侵袭。本课题研究目的在于进一步探索PKM2在肝癌细胞增 殖与凋亡方面的调控作用及相关分子生物学机制。 采用短发夹RNA转染HepG2和Huh7两株肝癌细胞株 ...
谌小春
core
CDK5 promotes HCC genesis and promotes cell migration by TPX2 [PDF]
, 2016 我国是世界上肝癌最高发的国家之一，由于其较高的发病率和致死率，严重威胁人民的健康，但目前大多数肝癌患者仍难以获得早期诊断及有效的治疗。细胞周期蛋白依赖性激酶5(cyclin-dependentkinase5，CDK5)是周期素依赖蛋白激酶家族的特殊成员，近年来发现其在肿瘤发生发展中起着关键的作用。CDK5通过与激活因子p35或p39结合后活化并磷酸化一系列下游分子，参与肿瘤细胞增殖、迁移、粘附、凋亡、细胞周期调控等。但CDK5对肝癌细胞功能的影响及其在肝癌中的调控机制还不清楚 ...
王付强
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