【目的】 探讨膜反向斑点杂交法在广东人G6PD基因6种常见突变同步检测中的应用【方法】 用高铁血红蛋白还原试验和G6PD/6-PGD酶活性比值法进行G6PD缺乏症筛查应用多重PCR方法扩增G6PD基因目的片段用Premier Primer6.0软件设计和优化针对广东人常见6种G6PD基因突变型的特异性寡核甘酸探针,用乙基-3-二甲氨基丙碳二亚胺(EDC)法处理尼龙膜并固定探针,制成G6PD基因突变检测用膜条多重PCR扩增产物与固定在膜条上寡核苷酸探针进行杂交 ...
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